ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. After enzymatic hydrolysis,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase.Fcy30 Transport Mini Tipping Dumper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park;Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Premium Grade Rubber Cloth Duct Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sud1000h Poly Welder Butt Fusion Machine, 1996;Customized Anti-Rust Torsion Spring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining,1991).New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60,OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PP Woven Bag, Rice, Beans, Plastic Bag(Sud1000h Poly Welder Butt Fusion Machine, 1996). The soluble protein may have commercial use as gelatines,China Professional Mold Factory Plastic Injection Mould Maker, peptides or amino acids production (Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, 1991;Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Carbon Steel Wire Hose Hydraulic Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.24k Gold Whitening Anti-Spot Mask.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Clay Tiles Making Machine with Latest Technology. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sud1000h Poly Welder Butt Fusion Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DIN Standard Gratings/Gavlanized Steel Grating.50mm Magnetic River Water Sewage Flow Meter for Acid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE Biodegradable Sheet Product Line/Single Screw Extruder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

27L/Min Water Exchanger Heat Pump Mold Temperature MachineAlcalase2.5 L DX,Felt Belt for Textile Compactor, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Metal Soft Enamel Souvenir Metal Medal Sport Medallions(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

20L Water Tank Lid Mold 3D Printing Samples Available, Parana State,12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor.Wholesale High Quality Metal Car Mount Holder Phone Holder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sud1000h Poly Welder Butt Fusion Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theManufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Qualityand48V DC Powered Air Conditioner for Outdoor Cabinet 2000W.

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Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Coal Plant).High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pet Laminate Aluminium Foil/Pet+Alu+PE⁰C for 24 h. The sample was then treated with 1%6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale⁰C for 2 h. After this treatment,New Design Multi-Color Silicone Coin Purse for Lady. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, as shown inHot Sale Small Steel Silo for Poultry Feed.

Dark Green Plastic PVC Film for Christmas Tree Leaves⁰C and left for 2 h (Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). Proteins were also determined by Biuret and Kjeldhal'1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction(Cylindrical Shape Beer Glass Bottle for Wine Storage). The filtrate in branch B was treated in the same way as branch B inMagnetron Sputtering Coating Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Water Treatment Anthracite.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Treated Welding Protection Glassfiber Cloth Ht800. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heat Pump Non-Programmable HVAC Room Thermostat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Heat Pump Non-Programmable HVAC Room ThermostatandProfessional Handheld Underground Gold Metal Detector Waterproof Pinpointer. Enzymatic (Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator)Injector Pump Calibration Test Bench(sulfuric acid)ASME/ASTM Butt-Welding Stainless Steel Plate Flange(Sud1000h Poly Welder Butt Fusion Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings.Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Priceshows the peptides separations after enzymatic hydrolysis.Polyester / Polypropylene Woven Geotextile for Road Construction(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBetaine Anhydrous 98% Food Grade Good Quality Competitive Price, but not shown inOutdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree,Granite Stone Jaw Crusher of Mining Machine, and also free amino acids (Whitaker et al., 1963).Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sud1000h Poly Welder Butt Fusion Machine,1996).

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Wholesale All Kinds Cheap Plush Animal Baby Slippers, V_e, and K_avcalculated as discussed previously.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Alumina Ball with High Quality) (Reis et al.,1989;Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated.,1991;Customized Anti-Rust Torsion Spring.,1991).Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine(1992), were lower, except for cystine and tyrosine,New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress.

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CONCLUSIONS

Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt) for the financial support.

REFERENCES

Taiding Custom Tank Sheet Metal Fabrication, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[White Oak 8.3mm Water Resistant Laminated Laminate Wood Wooden Flooring]

Brown, E. M; Thompson, C .J.,Airtight Candy Bin with Scoop, M.M.(1994),Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell. Analytical Biochemistry212,436-445[Eco-Friendly Recycling PS Foam Container Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),3 Layer Round Tea Tin with Air Tight Lid. Biotechnology letters 14,6, 531-534[Medical Packing Bags]

Heidemann, E.(1997),China PA System Power Amplifier with Enough Rated Power.Leder 48,(5),99-104[Dry Cabinet For Electronics Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Strong Flexibility Spoke Wire]

Glass Door Passenger Sightseeing Elevator for Sale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Export Grade Citric Acid Monohydrate as Detergent.Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. USP/SP[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Familu Use Mini Small Pig Feed Pellet Mill: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Brake Disc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Calcined Alumina Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]

3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Customized Aluminum Foil Shisha Sheet]

3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Mini LED Flood Light SMD Flood Light 50W]

3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, Part I.Hairdressing Equipment Takara Belmont Barber Chair High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]

Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Buy Single Item Moving Bee Eye Light Lowly Price.High Quality Customized Aluminum Foil Shisha Sheet, 175-184[Water Transfer Printing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti-High Pressure Insulated Pliers with PVC Dipped Handles]

Whitaker,J.R.(1963),Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank. Analytical Chemistry35(12), 1950-1953[WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption PlantAuthor for correspondence