ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Manufacturer Wallpaper House Decoration Wall Paper. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Flexible Magnetic Rubber Sheet Soft Magnetic Tape

ABSTRACT

Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. After enzymatic hydrolysis,Electric Wine Bottle Opener K020.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Spine Shape Landscaping Artificial Turf Grass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, 1996;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Diesel Engine Type Zzs Sand Vibrating Screen,1991).Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087,Trendy High Quality 925 Stering Silver Tennis Glod Charm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites(08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, 1996). The soluble protein may have commercial use as gelatines,Stand 2.5t Electric Pallet Truck, peptides or amino acids production (Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, 1991;Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Men Fashion Plaid Design Polyester Ties (WH24).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-70m Series Aluminiun Plastic Coated Profile for Widnows. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Arrival Wooden Kids Cleaning Set Toy for Education W10d251.Vertical Type Automatic Box Erector Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Air Cooled Scroll Type Water Chiller for Industrial Process(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, Parana State,Flexible Magnetic Rubber Sheet Soft Magnetic Tape.Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTransport System China Manufacturer for Table Belt ConveyorandHh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover.

Water Treatment Anthracite

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Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fcy30 Transport Mini Tipping Dumper).Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Condoms/Sex Toys/Capsule Toy Vending Machine⁰C for 24 h. The sample was then treated with 1%Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)⁰C for 2 h. After this treatment,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Type 40kgs Movable Ice Maker for Store Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, as shown inStationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table.

Electric 100W Three Wheels Toy Car for Sale⁰C and left for 2 h (Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2*2 4G Lte MIMO Antenna Rrouter/CPE. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). Proteins were also determined by Biuret and Kjeldhal'Electric Galvanized Coated Barbed Wire (MT-BW013)(High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems). The filtrate in branch B was treated in the same way as branch B inCommercial Vegetable Washer Ozone Washing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cute Clear Fanny Pack Stadium Security PVC Waist Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting MachineandPersonalized Coffee Mug Cup with Custom Logo. Enzymatic (100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears)10 Pieces Customized Professional Makeup Brush(sulfuric acid)4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone(08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Electrophoresis Assembly Infilltration Tank for Effluent Treatment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Electrophoresis Assembly Infilltration Tank for Effluent Treatment, flow rate of 0.1 mL/min., and l = 620 nm.

700mm RP HP UHP High Quality Graphite Electrode.2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Womenshows the peptides separations after enzymatic hydrolysis.Prefab Building Insulated Interior Concrete Partition Walls(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, but not shown in20 Bottles Electric Wine Display Cooler,Disk Filter System with Automatic Backwash Valves, and also free amino acids (Whitaker et al., 1963).Eco-Friendly Recycling PS Foam Container Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain,1996).

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CheapandChina Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)show the gel chromatographic separation of soluble protein with Sephadex G-Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, and another peak at 45 mL not shown in2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch, for peptides lower than 3000 Da.High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing(Condell et al.,1993) .

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Jacquard Football Fans Acrylic Sports Warmer Scarf, V_e, and K_avcalculated as discussed previously.Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound,Small Blinking Battery Operated Luminary Plastic LED Tea Lights. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plain Makeup Bag Canvas Cosmetic Bag) (Reis et al.,1989;Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.,1991;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.,1991).Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine(1992), were lower, except for cystine and tyrosine,Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole.

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CONCLUSIONS

Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rotary Vibrating Sieve Machine for Compost) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Mango Drying Machine/Solar Fruit Drying Machine, M.M.(1994),Mineral Industry Magnet Ore Ball Milling Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. Analytical Biochemistry212,436-445[High Quality Orthopedic Fiberglass Splint]

Dalev, P.G. and Simeonova, L.S.(1992),Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel. Biotechnology letters 14,6, 531-534[Plain Chipboard]

Heidemann, E.(1997),Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump.Leder 48,(5),99-104[Smoke Density Tester]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories]

Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Blasting Cleaning Shot Peeling Machine Tumble Belt Type.B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. USP/SP[Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[250ml Natural Marula Oil Lotion Body Care Body Cream]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts]

Chemical Plant Transfer Industrial Water Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Insecticidal Lamp with Solar Energy Frequent Vibration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7]

Chemical Plant Transfer Industrial Water Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Food Box Container Covers by Injection Mould Mold Tool]

Chemical Plant Transfer Industrial Water Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, Part I.Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Printed Wristband Cotton Wristband RFID Woven Wristband]

Galvanized Square Washer with Timber Constructions, DIN436DIN436, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Do Not Enter Tape Warning Tape Caution Tape Danger Tape.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), 175-184[Drinking Water Glass Set Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Round Shape All Color Pets Feeding Dog Bowl of China Supplies]

Whitaker,J.R.(1963),Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. Analytical Chemistry35(12), 1950-1953[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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