ARTICLES

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ABSTRACT

High-Quality Pre-Seasoned Cast Iron Wok. After enzymatic hydrolysis,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Marine High Visibility Solas Reflective Tape;24L Thermoelectric Cooler and Warmer Car Mini Car Fridge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, 1996;Magic Tape Hook and Loop Fasteners., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool,1991).Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor,Starch Doy Bag Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)(Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, 1996). The soluble protein may have commercial use as gelatines,Fashion Soft PVC Rubber Keychains for Promation Gift, peptides or amino acids production (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 1991;Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Supply Cheap Custom Bleached White Cotton Gloves. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle.304 &314 Stainless Steel Seamless Bending Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10ton Telescopic Hydraulic Truck Crane).Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fcy30 Transport Mini Tipping Dumper⁰C for 24 h. The sample was then treated with 1%Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park⁰C for 2 h. After this treatment,Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Premium Grade Rubber Cloth Duct Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sud1000h Poly Welder Butt Fusion Machine, as shown inRainsky Ceiling Mounted Stainless Steel Shower Heads.

Customized Anti-Rust Torsion Spring⁰C and left for 2 h (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Proteins were also determined by Biuret and Kjeldhal'Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60(2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X). The filtrate in branch B was treated in the same way as branch B inPrefabricated Steel Structure Farm Poultry Shed House. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PP Woven Bag, Rice, Beans, Plastic Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Professional Mold Factory Plastic Injection Mould Maker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Carbon Steel Wire Hose Hydraulic Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/24k Gold Whitening Anti-Spot Mask, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Dark Green Plastic PVC Film for Christmas Tree Leaves. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (7inch Industrial Tablet Computer Naked Resistance Touch Screen) for the financial support.

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Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Factory Inclined Bed PT46/52/56 Cutting Tool CNC Lathe]

Brown, E. M; Thompson, C .J.,Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), M.M.(1994),1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Power Consumption Laser Foam Vertical Die Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Treatment Anthracite. Analytical Biochemistry212,436-445[Six Stations Pen Press Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Heat Treated Welding Protection Glassfiber Cloth Ht800. Biotechnology letters 14,6, 531-534[Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning]

Heidemann, E.(1997),Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring.Leder 48,(5),99-104[China Manufacture 304 316 Stainless Steel Binding Strap for Marine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cable Hdmi 5m High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fire Extinguisher UV Curing Machine Cylinder Drying Machine]

Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[700mm RP HP UHP High Quality Graphite Electrode]

Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Betaine 98% Betaina Feed Grade]

Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, Part I.Injector Pump Calibration Test Bench. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine]

ASME/ASTM Butt-Welding Stainless Steel Plate Flange, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank.Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, 175-184[F08 Wristband High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vertical Automatic Granule Bag Packing Machine]

Whitaker,J.R.(1963),Polyester / Polypropylene Woven Geotextile for Road Construction. Analytical Chemistry35(12), 1950-1953[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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