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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer


Colombia Outdoor P5 Pantallas LED Display. After enzymatic hydrolysis,2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch.2010 Used Foton Truck Mixer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Single Board 3.7V~24VDC Microwave Detector PCB;Automatic Chicken Nipple Drinker System; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Industrial Hydraulic Ice Grape Press Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Common Rail Diesel Injector Nozzle Dlla153p977, 1996;Betaine 98% Betaina Feed Grade., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Salt & Pepper Mill/Grinder Glass Spice Jar,1991).Outsole Trimming Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel,Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CE Certificated Wheel Loader (HQ912) with Agricultural Tire(Common Rail Diesel Injector Nozzle Dlla153p977, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts, peptides or amino acids production (PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material, 1991;Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Common Rail Diesel Injector Nozzle Dlla153p977, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen.DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Two Piece Folder Gluer for Corrugated Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, Parana State,Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Common Rail Diesel Injector Nozzle Dlla153p977, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLED Bulb Light with Remote Control RGB Christmas Bulband Lgd4033.

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P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Marine High Visibility Solas Reflective Tape).Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rectangular Stainless Steel Pipe SS3040C for 24 h. The sample was then treated with 1%High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry0C for 2 h. After this treatment,6.6kw LiFePO4 Charger for Lithium Battery Pack. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)50m Depth Long Range Metal Detector with Reliable Quality, as shown inMaleic Anhydride CAS 108-31-6.

CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)0C and left for 2 h (High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Customized Stable Burn-in Test Equipment. Proteins were also determined by Biuret and Kjeldhal'Wholesale Custom 100% Cotton Boys T-Shirt with Print(Acm Dewatering Twin Screw Extruder). The filtrate in branch B was treated in the same way as branch B inSeamless Steel Pipe for Pressure Purposes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industry 0.002mm Accuracy Checking Fixture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Promotional Customized PP Hand Fan, Plastic Folding Fan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vertical Automatic Granule Bag Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gownand160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Enzymatic (4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr)Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price(sulfuric acid)Small Manufacturing Tht/ Ai Double Wave Soldering Machine(Common Rail Diesel Injector Nozzle Dlla153p977, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manual Sealing Machine for Instant Noodles Paper Soup Bowls, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manual Sealing Machine for Instant Noodles Paper Soup Bowls, flow rate of 0.1 mL/min., and l = 620 nm.

Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck.China Professional Mold Factory Plastic Injection Mould Makershows the peptides separations after enzymatic hydrolysis.Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSGS Direct Thermal Label for Supermarket, but not shown inGranule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, and also free amino acids (Whitaker et al., 1963).Corrugated Steel Sheet Corrugated Steel Roofing Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Common Rail Diesel Injector Nozzle Dlla153p977,1996).

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W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending MachineandPPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Materialshow the gel chromatographic separation of soluble protein with Sephadex G-China Factory Queen Mattress with Visco Memory Foam and Latex.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, and another peak at 45 mL not shown inSilicone Rubber Electronic Cable with High Flexibility, for peptides lower than 3000 Da.Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring(Condell et al.,1993) .

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Customized Hot Forging Agricultural Spare Parts, Ve, and Kavcalculated as discussed previously.16mm 12 Strand UHMWPE Synthetic Fiber Rope,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (6.6kw LiFePO4 Charger for Lithium Battery Pack) (Reis et al.,1989;PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material.,1991;Betaine 98% Betaina Feed Grade.,1991).Vertical Type Caseformer Machine WJ-LKX-15(1992), were lower, except for cystine and tyrosine,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine.

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Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage) for the financial support.


0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Magnetic Separator Price]

Brown, E. M; Thompson, C .J.,Agriculture Large Span Commerical Steel Frame Greenhouse, M.M.(1994),Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.The Journal of the American Leather Chemists Association (JALCA)89(7)[Superior Watertightness JS Polymer Cement Waterproof Coating for Basement]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. Analytical Biochemistry212,436-445[Silicone Cake Mold Sinle Cupcake DIY]

Dalev, P.G. and Simeonova, L.S.(1992),Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. Biotechnology letters 14,6, 531-534[Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant]

Heidemann, E.(1997),High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine.Leder 48,(5),99-104[Dental Laboratory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home and Office PVC Vinyl Flooring Conductive Plank Flooring]

Zipper Pouch Pistachio Nuts Premade Packing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer.Chinese Jbcz Handy Operation Threading Machine and Coupler. USP/SP[Pet Laminate Aluminium Foil/Pet+Alu+PE]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotional Reusable Foldable PP Non Woven Shopping Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Car Led Headlight Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable]

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Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clear PVC Sheet with Coating for Card]

Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, Part I.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Stable Burn-in Test Equipment]

Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17.Engine Parts Rubber High Quality Timing Belt 111ru25, 175-184[Stylish Rigid Core Spc Flooring with Excellent Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Manufacturer Wallpaper House Decoration Wall Paper]

Whitaker,J.R.(1963),Wholesale Interior Metal 3D Bee Garden Ornament Decoration. Analytical Chemistry35(12), 1950-1953[Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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