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ABSTRACT

Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. After enzymatic hydrolysis,Familu Use Mini Small Pig Feed Pellet Mill.3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer;Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hairdressing Equipment Takara Belmont Barber Chair High Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), 1996;Buy Single Item Moving Bee Eye Light Lowly Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Customized Aluminum Foil Shisha Sheet,1991).Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment,Rectangular Box Facial Tissue Paper Fk-110,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), 1996). The soluble protein may have commercial use as gelatines,Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, peptides or amino acids production (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1991;CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Q32 Series Tumble Belt/Crawler Shot Blasting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Bar Grating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Custom Self Adhesive Leather Patch for Jackets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale.380mm Working Area Concrete Diamond Floor Edge Grinder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Sheet Metal Stamping Bending Product Manufacturing Service, Parana State,Export Grade Citric Acid Monohydrate as Detergent.Waterproof EVA Rubber Roofing Material for Shingle Building, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCS1363 a Standard UK 3 Pin Power Plugand Elastic Tape.

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OEM Gray Cast Iron Sand Casting Products with Universal Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Bulb Light with Remote Control RGB Christmas Bulb).U. S. Type Drop Forged Wire Rope Clip(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Humizone Seaweed Customized Organic Liquid Fertilizer⁰C for 24 h. The sample was then treated with 1%Zipper Fabric Travel Passport Wallet Organiser⁰C for 2 h. After this treatment,Hot Sale Instax Mini Camera Korea Style Photo Album. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Low Profile Adjustable Bracket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, as shown in Lgd4033.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Round Type Twin Screw Extruder Barrel Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avASA-UPVC Anti-Corrosive Composite Roof Tile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sexy Mesh Transparent Female Running Tank Top with Hood Custom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Color Cardstock Color Paper Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Maleic Anhydride CAS 108-31-6andHome and Office PVC Vinyl Flooring Conductive Plank Flooring. Enzymatic (Precision Engineering Parts Bearing Support Unit for Ball Screw)LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection(sulfuric acid)165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man(Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Silicone Cake Mold Sinle Cupcake DIY, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Silicone Cake Mold Sinle Cupcake DIY, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

T Series Bevel Helical Gearboxes for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input) for the financial support.

REFERENCES

Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe]

Brown, E. M; Thompson, C .J.,Colorful PVC Foam Board with High Density and Customizable Size, M.M.(1994),Air Vent Diffuser Adjustbable Air Well and Jet Diffuser.The Journal of the American Leather Chemists Association (JALCA)89(7)[6.6kw LiFePO4 Charger for Lithium Battery Pack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Folding Aluminium Easy Move Choral Stage with Wheels for School]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Original Stainless Steel Singer Brand Sewing Machine Needles. Analytical Biochemistry212,436-445[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

Dalev, P.G. and Simeonova, L.S.(1992),Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. Biotechnology letters 14,6, 531-534[Superior Watertightness JS Polymer Cement Waterproof Coating for Basement]

Heidemann, E.(1997),Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance.Leder 48,(5),99-104[Stainless Steel Flanged Multi-Level Metal Seal Butterfly Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

3 Layer Round Tea Tin with Air Tight Lid, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve.Steel Bar Peeling Lathe Machine. USP/SP[Silicone Cake Mold Sinle Cupcake DIY]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chemical Product Sodium Gluconate Used for Concrete Admixture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Home and Office PVC Vinyl Flooring Conductive Plank Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale More Colors African Sequins Lace Fabric for Dress]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pet Laminate Aluminium Foil/Pet+Alu+PE]

Adding Water Rice Pulping Machine/Soybean Grinding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure]

Adding Water Rice Pulping Machine/Soybean Grinding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing]

Adding Water Rice Pulping Machine/Soybean Grinding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, Part I.Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable]

Hanging Necklace Fan USB Rechargeable Battery Operated Fan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Mushroom Rround Head Solid Rivet (Round head).Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 175-184[China Inflatable Rubber Mandrel for Making Culvert]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Foil Heat Shield Screen Matting Adhesive Backed]

Whitaker,J.R.(1963),Warm Women Suede Leisure Snow Boots for Winter (FF-1). Analytical Chemistry35(12), 1950-1953[Clear PVC Sheet with Coating for Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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