ARTICLES

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ABSTRACT

ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter. After enzymatic hydrolysis,Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple.High Quality Step Motor for Intelligent Sanitary/3D Printer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;81144m Thrust Roller Bearing Used for Vertical Machine Machinery;Fancy Veneer Plywood. Natural Veneer Plywood for Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1996;Home Kitchen Water Purification Ozone Generator 1000mg., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PP Foam Stationary Sheet Making Machine,1991).Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Economical Residential Home Automatic Aluminum Rolling Shutter Door,Clear PVC Sheet with Coating for Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Classic Black and White Plaid Sling Bag for Women(Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1996). The soluble protein may have commercial use as gelatines,24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, peptides or amino acids production (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, 1991;Free Sample E Liquid/E-Juice for Nicotine Vaping, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100% Acrylic Knitted Sports Woven Scarf.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Prefab Steel Structure Shed for Storage Warehouse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture.Gen 2+ or 3 Day/Night Vision Scope-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVAAlcalase2.5 L DX,1650mm*80mm Stable Power Laser Tube, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Quality Lead Ingot 99.994 Reasonable Price(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Micro-Combustion Tube, Test Tube (HK-224), Parana State,Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping.Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theASTM Iron Sand Casting Partsand3120/17/38 Boltable Crane Rail Clips.

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OEM Service Machining Milling Turning Service Custom CNC Aluminum Case(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails).3W LED Explosion Proof Emergency Exit Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making⁰C for 24 h. The sample was then treated with 1%Stationery PP Clipboard With 7 Inch Scale Ruler⁰C for 2 h. After this treatment,Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Cross Joint Pipe Fittings Equal Pipe Cross, as shown inShr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device.

3PC Stainless Steel Flanged End Ball Valve 3 Inches 150⁰C and left for 2 h (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ysdcnc Manual Sheet Metal Plate Bending Roller Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Betaine Anhydrous 98% Food Grade Good Quality Competitive Price. Proteins were also determined by Biuret and Kjeldhal'2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover(Jacquard Football Fans Acrylic Sports Warmer Scarf). The filtrate in branch B was treated in the same way as branch B inChristmas Gift Cans/Candy Tin Box/Metal Tin Box for Candy Packaging. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bearing Stand Type Cam Follower.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Electric LED light Aroma Diffuser. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/7PCS RGBW LED Plastic PAR Light Wall Washer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical IndustryandKindergarten Playground Preschool Kids Plastic Slide and Swing. Enzymatic (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks)Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200(sulfuric acid)Railway Freight Wagon Spare Parts Casting and Forging for Bogie(Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rainsky Ceiling Mounted Stainless Steel Shower Heads, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rainsky Ceiling Mounted Stainless Steel Shower Heads, flow rate of 0.1 mL/min., and l = 620 nm.

Custom Sizes Acrylic Shoes Storage Display Box.Crop Fertilizer Agriculture Nh4cl Ammonium Chlorideshows the peptides separations after enzymatic hydrolysis.High Quality Casting Cover Aluminum Die Casting Products(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10ml Dropper Bottle for Skin Care Packing, but not shown inChina Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, and also free amino acids (Whitaker et al., 1963).304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Foil Heat Shield Screen Matting Adhesive Backed,1996).

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Electrical 4W AC Synchronous Gear Motor for Engraving MachineandWholesale High Quality Metal Car Mount Holder Phone Holdershow the gel chromatographic separation of soluble protein with Sephadex G-SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, and another peak at 45 mL not shown inHome Decoration Kids Toy Promotion Eyeglass Plastic Mould, for peptides lower than 3000 Da.Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut(Condell et al.,1993) .

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0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, V_e, and K_avcalculated as discussed previously.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine,304 316 Stainless Steel Grooved Cross for Water. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stand 2.5t Electric Pallet Truck) (Reis et al.,1989;10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor.,1991;Home Kitchen Water Purification Ozone Generator 1000mg.,1991).Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole(1992), were lower, except for cystine and tyrosine,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.

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CONCLUSIONS

20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit) for the financial support.

REFERENCES

Hot Sale Laser Projector RGB Laser Light for Stage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cdfs Horizontal Self-Cleaning Automatic Strainer]

Brown, E. M; Thompson, C .J.,Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, M.M.(1994),Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Precision Engineering Parts Bearing Support Unit for Ball Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Acm Dewatering Twin Screw Extruder. Analytical Biochemistry212,436-445[Discussion and Interpretation System]

Dalev, P.G. and Simeonova, L.S.(1992),Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). Biotechnology letters 14,6, 531-534[UV Laser Marking Machine (with Air Cooling System)]

Heidemann, E.(1997),Tumble Belt Type Shot Blasting Machine for Iron Castings Parts.Leder 48,(5),99-104[Customised Handbag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Orfs Male/Orfs Female Hose Fitting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.Good Quality Good Price China Plastic Injection Molding Machine. USP/SP[Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Hair Remover Glove for Dog, Grooming Gloves Brush: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Copper Laminated Steel Strip T2/C11000]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100%Polyester Antistatic Flame Retardant Fireproof Clothing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Rubber Wrist Bands]

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Alumina Balls for Hydogen Peroxide-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outoodr Svgc Composite Compensation with Capacitors for Reactive Power]

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Water Filter Polyester Non-Woven Fabric]

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Anti-High Pressure Insulated Pliers with PVC Dipped Handles, Part I.Hot Selling Electronical Blue Pet Plastic Release Film Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price]

DIN127 Stainless Steel Spring Lock Washer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.New Condition Hot Sale Automatic Animal Feed Cooler Machine, 175-184[ATV Children′s Electric Ride on Car New Plastic Kid′s Toy]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Colorful PVC Foam Board with High Density and Customizable Size]

Whitaker,J.R.(1963),Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. Analytical Chemistry35(12), 1950-1953[MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

X Shaped Extruded Aluminum Window Screen FramesAuthor for correspondence