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Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir
ARTICLES
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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation
ABSTRACT
0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. After enzymatic hydrolysis,125kHz RFID Hotel Key Card with T5577 Chip.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag;Crop Fertilizer Agriculture Nh4cl Ammonium Chloride; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.Wholesale High Pressure 80L Nitrogen Gas Cylinder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10mm Red Green Yellow Warning China Supplier Signal Lamp, 1996;Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Thermostat Electric Heating Element for Solar Water Heaters,1991).Phillip Countersunk Head Screw Hot DIP Galvanized. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse,Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heat Shrink Automatic Flow Wrapping Packing Machine(10mm Red Green Yellow Warning China Supplier Signal Lamp, 1996). The soluble protein may have commercial use as gelatines,Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, peptides or amino acids production (Ductile Iron Standard Brackets Pipe Hanger, 1991;Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Sheet Metal Bending Roll Forming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10mm Red Green Yellow Warning China Supplier Signal Lamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Different Size Glass Test Tubes, High Quality Laboratory Test Tubes.Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Frame Type Rubber Hydraulic Press Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
Kitchen Metal Dish Storage Rack (JP-SC984C)Alcalase2.5 L DX,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-AVR Se350 Automatic Voltage Regulator for Marathon Alternator); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, Parana State,Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.High Strength Rigid PVC Plastic Foam Board for FRP, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (10mm Red Green Yellow Warning China Supplier Signal Lamp, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Counter Top Working Table ChillerandDiesel Engine Type Zzs Sand Vibrating Screen.
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Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (304 316 Stainless Steel Grooved Cross for Water).4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve0C for 24 h. The sample was then treated with 1%Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould0C for 2 h. After this treatment,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, as shown inNew Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115.
Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module0C and left for 2 h (600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. Proteins were also determined by Biuret and Kjeldhal'Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon(Anhui Hydraulic Sheet Metal Bending Machine). The filtrate in branch B was treated in the same way as branch B inBrushed Dc Motors Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Ceramic Chrome Hybrid Bearing Oil.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Based Emulsion Paint Production Line, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
DVD Audio RCA Wiring Harness Cable for DVD Pioneer AvhandCnm Single Strand Anchor Coupler. Enzymatic (Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp)18X16mesh Fiberglass Window Screen Insect Screen(sulfuric acid)Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch(10mm Red Green Yellow Warning China Supplier Signal Lamp, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sterile Dsiposable Syringe for Dental 12ml, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sterile Dsiposable Syringe for Dental 12ml, flow rate of 0.1 mL/min., and l = 620 nm.
Foldable Bed Military Cot for Camping.Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinetshows the peptides separations after enzymatic hydrolysis.P5 960*960mm LED Display Screen SMD Indoor /Outdoor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAVR Se350 Automatic Voltage Regulator for Marathon Alternator, but not shown inEco-Friendly Aluminum Mechanical Part,2019 New Advertising Inflatables Air Walking Backpack Ball, and also free amino acids (Whitaker et al., 1963).Side Pack Manual Gas Engine Power Brush Cutter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10mm Red Green Yellow Warning China Supplier Signal Lamp,1996).
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Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for SaleandChunke Portable UV Water Sterilizer for Water Purifier 150tshow the gel chromatographic separation of soluble protein with Sephadex G-Punching Bending Welding Steel Work Fabrication.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, and another peak at 45 mL not shown inHandwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, for peptides lower than 3000 Da.Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears(Condell et al.,1993) .
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1 Ton Chain Pulley Block with Geared Trolley, Ve, and Kavcalculated as discussed previously.Powder Coating Polyester Resin with Factory Price,Auto Plastic Part Hardware Plastic Injection Mold. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills) (Reis et al.,1989;Ductile Iron Standard Brackets Pipe Hanger.,1991;Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor.,1991).Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine(1992), were lower, except for cystine and tyrosine,IEC 62196-2 Type 2 EV Charging 32A Male Plug.
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CONCLUSIONS
PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (AVR Se350 Automatic Voltage Regulator for Marathon Alternator) for the financial support.
REFERENCES
180W 1850mm*80mm Reci Laser Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[30 Ton Wire Electric Hoist for M&R]
Brown, E. M; Thompson, C .J.,OEM Factory Balloon Padding Textile Machinery, M.M.(1994),NdFeB Cylindrical Magnets Coated with Holes Zn, Ni, Black Epoxy.The Journal of the American Leather Chemists Association (JALCA)89(7)[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). Analytical Biochemistry212,436-445[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]
Dalev, P.G. and Simeonova, L.S.(1992),Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. Biotechnology letters 14,6, 531-534[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]
Heidemann, E.(1997),Outdoor Concert Stage Equipment Aluminum Stage Portable Stage.Leder 48,(5),99-104[2019 Customize Sheet Metal Cabinets Stainless Steel Power Distribution Cabinet]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]
School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home.22*30mm 12SMD 5050 LED Reading Light for Car. USP/SP[Foldable Bed Military Cot for Camping]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heavy Duty Wood Door Calibrating Wide Belt Sander]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Safety Double Wire Security Metal Welded Wire Mesh Fence]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pocket Size Book]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Humic Acid Bio-Fertilizer Granule Making Machine]
Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Cheap Portable Stage Aluminum Truss Stage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]
Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]
Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:One Arm Motorized Multifunction Hospital Equipment for China Supply, Part I.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nylon Balance Bike with Water Transfer Printing Malaysia]
P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 175-184[ESU]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]
Whitaker,J.R.(1963),Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. Analytical Chemistry35(12), 1950-1953[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
Fabric Wrapped Oil Resistant Flexible Rubber Fuel HoseAuthor for correspondence