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ABSTRACT

High Quality Mini LED Flood Light SMD Flood Light 50W. After enzymatic hydrolysis,Ferrite Core for Power Transformer (Ee16e).Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Manufacturer Wallpaper House Decoration Wall Paper;Flexible Magnetic Rubber Sheet Soft Magnetic Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric Wine Bottle Opener K020, 1996;Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery,1991).China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Spine Shape Landscaping Artificial Turf Grass,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board(Electric Wine Bottle Opener K020, 1996). The soluble protein may have commercial use as gelatines,Diesel Engine Type Zzs Sand Vibrating Screen, peptides or amino acids production (Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, 1991;Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Trendy High Quality 925 Stering Silver Tennis Glod Charm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stand 2.5t Electric Pallet Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric Wine Bottle Opener K020, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Air Cooled Scroll Type Water Chiller for Industrial Process, Parana State,Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681).Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Electric Wine Bottle Opener K020, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Frame Hydraulic Press Machine for Bending PriceandFamilu Use Mini Small Pig Feed Pellet Mill.

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Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17).Rotary Vibrating Sieve Machine for Compost(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud⁰C for 24 h. The sample was then treated with 1%Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine⁰C for 2 h. After this treatment,Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, as shown inType 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug.

Condoms/Sex Toys/Capsule Toy Vending Machine⁰C and left for 2 h (Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price. Proteins were also determined by Biuret and Kjeldhal'Outdoor Type 40kgs Movable Ice Maker for Store Use(Air Vent Diffuser Adjustbable Air Well and Jet Diffuser). The filtrate in branch B was treated in the same way as branch B in24v Food Grade Self-Priming Pump High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electric 100W Three Wheels Toy Car for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2*2 4G Lte MIMO Antenna Rrouter/CPE(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Galvanized Coated Barbed Wire (MT-BW013)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Disk Filter System with Automatic Backwash ValvesandMini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. Enzymatic (Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool)Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter(sulfuric acid)China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor(Electric Wine Bottle Opener K020, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cute Clear Fanny Pack Stadium Security PVC Waist Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cute Clear Fanny Pack Stadium Security PVC Waist Bag, flow rate of 0.1 mL/min., and l = 620 nm.

Personalized Coffee Mug Cup with Custom Logo.Outdoor Type 40kgs Movable Ice Maker for Store Useshows the peptides separations after enzymatic hydrolysis.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGood Quanlity Double Acting Stainless Steel Offset Butterfly Valve, but not shown inCantilever Oxygen Plasma Cutter 80,10 Pieces Customized Professional Makeup Brush, and also free amino acids (Whitaker et al., 1963).4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electric Wine Bottle Opener K020,1996).

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Disk Filter System with Automatic Backwash Valves, V_e, and K_avcalculated as discussed previously.Eco-Friendly Recycling PS Foam Container Production Line,Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)) (Reis et al.,1989;Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.,1991;Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.,1991).Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using(1992), were lower, except for cystine and tyrosine,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing.

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CONCLUSIONS

Jacquard Football Fans Acrylic Sports Warmer Scarf. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251) for the financial support.

REFERENCES

Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cartoon Embroidery Towel]

Brown, E. M; Thompson, C .J.,Small Blinking Battery Operated Luminary Plastic LED Tea Lights, M.M.(1994),Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top10 1080P H. 264 P2p CCTV WiFi IP Camera]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ CNC Lathe Machine Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole. Analytical Biochemistry212,436-445[Plastic Injection Molding for Auto Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket. Biotechnology letters 14,6, 531-534[PVC Profile with Net Making Machine Used for Window Sealing Profile]

Heidemann, E.(1997),High Precision Plastic Mold Manufacturer in China.Leder 48,(5),99-104[Aluminum Trolley Tools Case]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

Transport System China Manufacturer for Table Belt Conveyor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mango Drying Machine/Solar Fruit Drying Machine.Mineral Industry Magnet Ore Ball Milling Machine. USP/SP[Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Square Premium Glass Perfume Bottle 100ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zirconia Oxide Balls]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Price Printing Doy Pack Bag]

Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Xjm Series Rubber Extruding Machine]

Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 500m 100lb Super Strong Braided Fishing Line]

Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Blasting Cleaning Shot Peeling Machine Tumble Belt Type, Part I.B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light]

Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chemical Plant Transfer Industrial Water Pump.Insecticidal Lamp with Solar Energy Frequent Vibration, 175-184[IP67 Aluminum Limit Switch for Tower Crane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier]

Whitaker,J.R.(1963),Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286. Analytical Chemistry35(12), 1950-1953[OEM Factory Balloon Padding Textile Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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