ARTICLES

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ABSTRACT

Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. After enzymatic hydrolysis,China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery.China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Spine Shape Landscaping Artificial Turf Grass;08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diesel Engine Type Zzs Sand Vibrating Screen, 1996;Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bluetooth Smart Watch with Heart Rate Monitor for Men 72087,1991).Trendy High Quality 925 Stering Silver Tennis Glod Charm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites,Stand 2.5t Electric Pallet Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion(Diesel Engine Type Zzs Sand Vibrating Screen, 1996). The soluble protein may have commercial use as gelatines,Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, peptides or amino acids production (Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label, 1991;Men Fashion Plaid Design Polyester Ties (WH24), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;70m Series Aluminiun Plastic Coated Profile for Widnows., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Arrival Wooden Kids Cleaning Set Toy for Education W10d251.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vertical Type Automatic Box Erector Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diesel Engine Type Zzs Sand Vibrating Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Cooled Scroll Type Water Chiller for Industrial Process.Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, Parana State,Electric Wine Bottle Opener K020.Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Diesel Engine Type Zzs Sand Vibrating Screen, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connectorand60m3/H Lamella Clarifier for River Water Treatment.

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Condoms/Sex Toys/Capsule Toy Vending Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Starch Doy Bag Packaging Machine).Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price⁰C for 24 h. The sample was then treated with 1%Outdoor Type 40kgs Movable Ice Maker for Store Use⁰C for 2 h. After this treatment,Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric 100W Three Wheels Toy Car for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2*2 4G Lte MIMO Antenna Rrouter/CPE, as shown in2 Quick Change Flexible Roloc Disc.

2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)⁰C and left for 2 h (Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Galvanized Coated Barbed Wire (MT-BW013). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. Proteins were also determined by Biuret and Kjeldhal'Slotter Sheet Metal V Grooving Machine From Professional Manufacturer(Soldering Machine LED Module PCB Hot Air Soldering Station). The filtrate in branch B was treated in the same way as branch B inStreetbike Birmingham. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMining Slurry Dewatering Rotary Precision Disc Ceramic Filter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cute Clear Fanny Pack Stadium Security PVC Waist Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Personalized Coffee Mug Cup with Custom Logo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ground Finish Molybdenum Tzm Rodand10 Pieces Customized Professional Makeup Brush. Enzymatic (4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone)Electrophoresis Assembly Infilltration Tank for Effluent Treatment(sulfuric acid)700mm RP HP UHP High Quality Graphite Electrode(Diesel Engine Type Zzs Sand Vibrating Screen, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Prefab Building Insulated Interior Concrete Partition Walls, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Prefab Building Insulated Interior Concrete Partition Walls, flow rate of 0.1 mL/min., and l = 620 nm.

Disk Filter System with Automatic Backwash Valves. 20%Scshows the peptides separations after enzymatic hydrolysis.Eco-Friendly Recycling PS Foam Container Production Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManufacturer of Soda Ash Dense 99 2% Price, but not shown inBarbed Wire Right off The Border Wall to Protect Homes,Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, and also free amino acids (Whitaker et al., 1963).Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diesel Engine Type Zzs Sand Vibrating Screen,1996).

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Magnifying Mirror Shaving Mirror 2XandCAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powdershow the gel chromatographic separation of soluble protein with Sephadex G-High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing.Jacquard Football Fans Acrylic Sports Warmer Scarf, and another peak at 45 mL not shown inOutdoor Concert Stage Equipment Aluminum Stage Portable Stage, for peptides lower than 3000 Da.Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound(Condell et al.,1993) .

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Small Blinking Battery Operated Luminary Plastic LED Tea Lights, V_e, and K_avcalculated as discussed previously.Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine,Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery) (Reis et al.,1989;Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label.,1991;Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.,1991).Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket(1992), were lower, except for cystine and tyrosine,High Precision Plastic Mold Manufacturer in China.

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CONCLUSIONS

Mango Drying Machine/Solar Fruit Drying Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine) for the financial support.

REFERENCES

Mineral Industry Magnet Ore Ball Milling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Warning Barricade Yellow/Black Flame-Retardant Tape]

Brown, E. M; Thompson, C .J.,DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut, M.M.(1994),Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. Analytical Biochemistry212,436-445[Hot Sale Instax Mini Camera Korea Style Photo Album]

Dalev, P.G. and Simeonova, L.S.(1992),Blasting Cleaning Shot Peeling Machine Tumble Belt Type. Biotechnology letters 14,6, 531-534[Sterile Dsiposable Syringe for Dental 12ml]

Heidemann, E.(1997),B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine.Leder 48,(5),99-104[Rock Wool Composite Insulation Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Flexible Magnetic Rubber Sheet Soft Magnetic Tape, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap.Chemical Plant Transfer Industrial Water Pump. USP/SP[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Insecticidal Lamp with Solar Energy Frequent Vibration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PP Woven Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4′′-12′′ High-Efficient Circular Carbide Tct Saw Blade for Cutting Tool]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ CNC Lathe Machine Milling]

Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bending Sheet Metal Custom-Made Aluminum Bracket]

Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Square Washer with Timber Constructions, DIN436DIN436, Part I.Do Not Enter Tape Warning Tape Caution Tape Danger Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pneumatic Air Driven Liquid Booster Pump]

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws.Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 175-184[Alimina Donor Top Grade 9A 100% Virgin Indian Hair]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Engine Parts Rubber High Quality Timing Belt 111ru25]

Whitaker,J.R.(1963),Good Quality PVC Plastic Ceiling for Wall Panel. Analytical Chemistry35(12), 1950-1953[White HPL 600*600mm Coffee Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power PlantAuthor for correspondence