ARTICLES

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ABSTRACT

Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. After enzymatic hydrolysis,Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).Premium Grade Rubber Cloth Duct Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sud1000h Poly Welder Butt Fusion Machine;Customized Anti-Rust Torsion Spring; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 1996;Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic,1991).PP Woven Bag, Rice, Beans, Plastic Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Professional Mold Factory Plastic Injection Mould Maker,Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size(New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 1996). The soluble protein may have commercial use as gelatines,Industrial Carbon Steel Wire Hose Hydraulic Pipe, peptides or amino acids production (24k Gold Whitening Anti-Spot Mask, 1991;Clay Tiles Making Machine with Latest Technology, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN Standard Gratings/Gavlanized Steel Grating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.50mm Magnetic River Water Sewage Flow Meter for Acid.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE Biodegradable Sheet Product Line/Single Screw Extruder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰27L/Min Water Exchanger Heat Pump Mold Temperature Machine.Felt Belt for Textile Compactor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, Parana State,Fcy30 Transport Mini Tipping Dumper.Pet Laminate Aluminium Foil/Pet+Alu+PE, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUltrasonic Automatic Face Mask Blank Making MachineandOffice Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding.

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6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Top Quality Galvanized Iron Wire as Binding Wire).New Design Multi-Color Silicone Coin Purse for Lady(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065⁰C for 24 h. The sample was then treated with 1%Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen⁰C for 2 h. After this treatment,Dark Green Plastic PVC Film for Christmas Tree Leaves. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), as shown inAlumina Balls for Hydogen Peroxide.

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction⁰C and left for 2 h (Factory Stock Spring Steel U Type Clip Nut). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Water Treatment Anthracite. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heat Treated Welding Protection Glassfiber Cloth Ht800. Proteins were also determined by Biuret and Kjeldhal'Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring(Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2). The filtrate in branch B was treated in the same way as branch B inHands Free Balance Scooter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Pump Non-Programmable HVAC Room Thermostat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBeer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Injector Pump Calibration Test Bench, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing BoomandASME/ASTM Butt-Welding Stainless Steel Plate Flange. Enzymatic (500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank)Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings(sulfuric acid)Polyester / Polypropylene Woven Geotextile for Road Construction(New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Granite Stone Jaw Crusher of Mining Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Granite Stone Jaw Crusher of Mining Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor.Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlightshows the peptides separations after enzymatic hydrolysis.Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle, but not shown inProfessional Stainless Steel Ethanol Processing Plant From Cassava,The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, and also free amino acids (Whitaker et al., 1963).Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt,1996).

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CE Certificated Wheel Loader (HQ912) with Agricultural TireandHigh Aluminum Insulating Ceramic Fiber Paper for Industrial Kilnsshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesale All Kinds Cheap Plush Animal Baby Slippers.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car, and another peak at 45 mL not shown inFactory Price 6mm Stainless Steel Wire Rope 7*7, for peptides lower than 3000 Da.Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane(Condell et al.,1993) .

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Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, V_e, and K_avcalculated as discussed previously.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress,Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging) (Reis et al.,1989;24k Gold Whitening Anti-Spot Mask.,1991;Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.,1991).Taiding Custom Tank Sheet Metal Fabrication(1992), were lower, except for cystine and tyrosine,Airtight Candy Bin with Scoop.

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CONCLUSIONS

Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale High Quality Metal Car Mount Holder Phone Holder) for the financial support.

REFERENCES

Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SMT Spare Parts Ekra 300mm Squegee]

Brown, E. M; Thompson, C .J.,3 Layer Round Tea Tin with Air Tight Lid, M.M.(1994),China PA System Power Amplifier with Enough Rated Power.The Journal of the American Leather Chemists Association (JALCA)89(7)[Prefab Steel Structure Shed for Storage Warehouse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Export Grade Citric Acid Monohydrate as Detergent. Analytical Biochemistry212,436-445[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

Dalev, P.G. and Simeonova, L.S.(1992),Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. Biotechnology letters 14,6, 531-534[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

Heidemann, E.(1997),Familu Use Mini Small Pig Feed Pellet Mill.Leder 48,(5),99-104[Soft Plastic Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rebonded Foam Machina]

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light.Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. USP/SP[Vitamin B1 HCl 98% Feed Grade]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blue LLDPE Stretch Film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

Hairdressing Equipment Takara Belmont Barber Chair High Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

Hairdressing Equipment Takara Belmont Barber Chair High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Hairdressing Equipment Takara Belmont Barber Chair High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Buy Single Item Moving Bee Eye Light Lowly Price, Part I.High Quality Customized Aluminum Foil Shisha Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Low Profile Adjustable Bracket]

Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment.Rectangular Box Facial Tissue Paper Fk-110, 175-184[Distillation Column]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump]

Whitaker,J.R.(1963),SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. Analytical Chemistry35(12), 1950-1953[22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Airport Runway Reinforced PP Twist Fiber Toghter with Mesh FiberAuthor for correspondence