ARTICLES

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ABSTRACT

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. After enzymatic hydrolysis,Energy-Saving Biogas Double Stove for Sale.Valve Bag Dry Mortar Bagging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, 1996;PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine,1991).Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch,Custom Stainless Steel Jewelry Production by Metal Injection Molding Process,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Directly Selling Engine Standard Spark Plug(Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, 1996). The soluble protein may have commercial use as gelatines,Custom Logo Silicone Wristband, Rubber Wrist Bands, peptides or amino acids production (Custom All Kinds of Acrylic Shoe Display Racks, 1991;Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Supply U40 Disposable Insulin Syringe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Water Chiller Air Cooled Chiller Industrial Chiller for Sale.Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Precision Tungsten Carbide Machine Customized Cutting Tool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Swimming Pool Water Disinfect Ozone Generator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (19mic Black Cast PE Stretch Film for Pallet Wrapping).20kv 50kVA High Voltage Dry Type Power Supply Transformer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp⁰C for 24 h. The sample was then treated with 1%High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter⁰C for 2 h. After this treatment,4 Styles Blue Glass Crystal Butt Beads Spiral Plug. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industrial Activated Carbon Water Filter Industry Water Treatment, as shown inImee Print Customized Printing Agenda Printing Nb21.

2016 New Generation Sausage Making Machine⁰C and left for 2 h (SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent. Proteins were also determined by Biuret and Kjeldhal'New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)(Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed). The filtrate in branch B was treated in the same way as branch B inYg8 Tungsten Carbide Bur. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Supplier Building Construction Steel Frame (ZY431).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLab Equipment portable Fume Hood(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10ton Telescopic Hydraulic Truck Crane⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV, pH 3.25., mobile phase B: buffer solution of 0.01 mol/for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2000mj Q Switched for Pigment Removal with Ce Certification) for the financial support.

REFERENCES

Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Senken HD1440p H22 IR Digital Police Mini Portable Body Worn Camera with GPS]

Brown, E. M; Thompson, C .J.,4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging, M.M.(1994),22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Reflective Hand Hold Thermoplastic Road Marking]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Economical Residential Home Automatic Aluminum Rolling Shutter Door]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD. Analytical Biochemistry212,436-445[2000mj Q Switched for Pigment Removal with Ce Certification]

Dalev, P.G. and Simeonova, L.S.(1992),Low Power Consumption Laser Foam Vertical Die Cutting Machine. Biotechnology letters 14,6, 531-534[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Activated Carbon Water Filter Industry Water Treatment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof EVA Rubber Roofing Material for Shingle Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Brass Pump Spare Parts for Circulating Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sheath Other series]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

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Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit, Part I.SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Counter Top Working Table Chiller]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Diesel Engine Type Zzs Sand Vibrating Screen]

Whitaker,J.R.(1963),Flat Surface Mounted 300mm 24W LED Panel Light for Interior. Analytical Chemistry35(12), 1950-1953[Condoms/Sex Toys/Capsule Toy Vending Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

304 316 Stainless Steel Grooved Cross for WaterAuthor for correspondence