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ABSTRACT

Fully Automatic C Z Purlin Cold Roll Forming Machinery. After enzymatic hydrolysis,Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Peristaltic Pump with Connecting The High Speed Tubular Separator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, 1996;LED Bulb Light with Remote Control RGB Christmas Bulb., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Home Decoration Kids Toy Promotion Eyeglass Plastic Mould,1991).Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Price Gi/PPGI Roofing Sheet in China,2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multipurpose Wireless PA Amplifier Lectern PA Speaker(High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, 1996). The soluble protein may have commercial use as gelatines,3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, peptides or amino acids production (Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, 1991;High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12ml Round Shape Empty Clear Nail Polish Glass Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.M4X10 Round Head Core Pulling Aluminum Nail.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Skytone 4 Channels 3500W Each DJ Power Amplifier.Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, Parana State,Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.Flexible PVC Granules for Cable, Wire and Insulation, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHerbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying MachineandChemical Plant Transfer Industrial Water Pump.

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7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector).Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brass Pump Spare Parts for Circulating Pump⁰C for 24 h. The sample was then treated with 1%Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes⁰C for 2 h. After this treatment,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lantern for Garden Decoration, as shown in60m3/H Lamella Clarifier for River Water Treatment.

Bending Sheet Metal Custom-Made Aluminum Bracket⁰C and left for 2 h (DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Shacman F3000 6X4 Truck Trailer Head. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump. Proteins were also determined by Biuret and Kjeldhal'Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention(Starch Doy Bag Packaging Machine). The filtrate in branch B was treated in the same way as branch B inNatural Gas Modern Fire Pit. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cylindrical Shape Beer Glass Bottle for Wine Storage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Injection for OEM Product. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Activated Carbon Strong Effect Peeling off Face Mask Mud, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Type Price Prefabricated Roof Tile in Sudan, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2 Quick Change Flexible Roloc DiscandStackable Mahogany Color Wooden Chiavari Chair with Soft Cushion. Enzymatic (Factory Supply 100% Natural Epimedium Extract for Male Health)Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust(sulfuric acid)Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug(High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Top10 1080P H. 264 P2p CCTV WiFi IP Camera, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Top10 1080P H. 264 P2p CCTV WiFi IP Camera, flow rate of 0.1 mL/min., and l = 620 nm.

Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture.Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decorationshows the peptides separations after enzymatic hydrolysis.Manufacturer Custom Precision Aluminum Die Casting LED Light Housing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSoldering Machine LED Module PCB Hot Air Soldering Station, but not shown inGround Finish Molybdenum Tzm Rod,Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate, and also free amino acids (Whitaker et al., 1963).1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding,1996).

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Manufacturer of Soda Ash Dense 99 2% PriceandBarbed Wire Right off The Border Wall to Protect Homesshow the gel chromatographic separation of soluble protein with Sephadex G-Full Color Child Book Printing Service.1.5 Inch High Pressure Petrol/Gasoline Water Pump, and another peak at 45 mL not shown inNew Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, for peptides lower than 3000 Da.Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine(Condell et al.,1993) .

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Equal and Unequal High Quality Hot Rolled Steel Angle, V_e, and K_avcalculated as discussed previously.Halal Chicken Slaughter Machine for Sale,Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder) (Reis et al.,1989;Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.,1991;LED Bulb Light with Remote Control RGB Christmas Bulb.,1991).2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr(1992), were lower, except for cystine and tyrosine,Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8.

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CONCLUSIONS

3 PCS Headband for Baby Girls with Hair Bows. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Wine Carrier Bag Gift Box Tote) for the financial support.

REFERENCES

LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Food Additive Thickener Xanthan Gum 200 Mesh]

Brown, E. M; Thompson, C .J.,High Quality Xanthan Gum for Personal Care and Cosmetic Applications, M.M.(1994),Manual Vertical Car Key Cutting Machine for Locksmith.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape. Analytical Biochemistry212,436-445[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Dalev, P.G. and Simeonova, L.S.(1992),Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069). Biotechnology letters 14,6, 531-534[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

Chicken Layer Cage Poultry Cage Chicken Cages, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Price Welding Electrode (e7018/e7018-1).Ep/Nn/EPP Industrial Rubber Conveyor Belt. USP/SP[Hot Sale Instax Mini Camera Korea Style Photo Album]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sterile Dsiposable Syringe for Dental 12ml]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Deep Hole Drilling Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PP Woven Bag]

New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Spare Parts Ignition Coil Fp85-18-100c, Part I.vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Flooded Type Water Cooled Screw Chiller, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday.Enameled Mini Cast Iron Casserole, 175-184[Fenugreek Saponins 50%]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ CNC Lathe Machine Milling]

Whitaker,J.R.(1963),Good Quality Tomato Sauce Making Machine. Analytical Chemistry35(12), 1950-1953[Bending Sheet Metal Custom-Made Aluminum Bracket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pneumatic Air Driven Liquid Booster PumpAuthor for correspondence