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ABSTRACT

AAC Block Production Line / AAC Equipment Manufacturing/ Machinery. After enzymatic hydrolysis,CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine.Q32 Series Tumble Belt/Crawler Shot Blasting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Galvanized Bar Grating;High Quality Custom Self Adhesive Leather Patch for Jackets; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (380mm Working Area Concrete Diamond Floor Edge Grinder, 1996;Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate,1991).Discussion and Interpretation System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input,Frozen Soybean in Pod,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Sheet Metal Stamping Bending Product Manufacturing Service(380mm Working Area Concrete Diamond Floor Edge Grinder, 1996). The soluble protein may have commercial use as gelatines,Waterproof EVA Rubber Roofing Material for Shingle Building, peptides or amino acids production (OEM Gray Cast Iron Sand Casting Products with Universal Parts, 1991;U. S. Type Drop Forged Wire Rope Clip, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Humizone Seaweed Customized Organic Liquid Fertilizer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Zipper Fabric Travel Passport Wallet Organiser.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Instax Mini Camera Korea Style Photo Album. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (380mm Working Area Concrete Diamond Floor Edge Grinder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Low Profile Adjustable Bracket.Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Acrylic Beer Display Racks with LED(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Counter Top Working Table Chiller).Sexy Mesh Transparent Female Running Tank Top with Hood Custom(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Color Cardstock Color Paper Manufacturer⁰C for 24 h. The sample was then treated with 1%Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts⁰C for 2 h. After this treatment,Home and Office PVC Vinyl Flooring Conductive Plank Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Precision Engineering Parts Bearing Support Unit for Ball Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, as shown inDiesel Engine Type Zzs Sand Vibrating Screen.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Construction & Decoration ERW Square Steel Tube Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Excavator Radiator /Oil Radiator /Water Radiator Manufacture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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AVR Se350 Automatic Voltage Regulator for Marathon AlternatorandEco-Friendly Aluminum Mechanical Partshow the gel chromatographic separation of soluble protein with Sephadex G-Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, and another peak at 45 mL not shown in2*2 4G Lte MIMO Antenna Rrouter/CPE, for peptides lower than 3000 Da.Steel Bar Peeling Lathe Machine(Condell et al.,1993) .

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Chemical Product Sodium Gluconate Used for Concrete Admixture, V_e, and K_avcalculated as discussed previously.Adding Water Rice Pulping Machine/Soybean Grinding Machine,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chunke Portable UV Water Sterilizer for Water Purifier 150t) (Reis et al.,1989;OEM Gray Cast Iron Sand Casting Products with Universal Parts.,1991;Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam.,1991).Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing(1992), were lower, except for cystine and tyrosine,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire.

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CONCLUSIONS

Hanging Necklace Fan USB Rechargeable Battery Operated Fan. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, M.M.(1994),Warm Women Suede Leisure Snow Boots for Winter (FF-1).The Journal of the American Leather Chemists Association (JALCA)89(7)[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home Kitchen Water Purification Ozone Generator 1000mg]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality Xanthan Gum Application of Food with Best Price. Analytical Biochemistry212,436-445[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Dalev, P.G. and Simeonova, L.S.(1992),Wet Wire Drawing Machine for High Carbon Mattress Spring Wire. Biotechnology letters 14,6, 531-534[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]

Heidemann, E.(1997),120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome.Leder 48,(5),99-104[Maxdrill T38 70mm Practical New Style Hammer Drilling Tools]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

Rectangular Box Facial Tissue Paper Fk-110, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Effluent Treatment Ferrous Sulphate Heptahydrate 98%.Rechargeable 9V Li Ion Battery with Micro USB Charging Port. USP/SP[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Transport System China Manufacturer for Table Belt Conveyor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Interior Decoration Material Wholesale Wallpaper with High Grade]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Foldable Bed Military Cot for Camping]

Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heavy Duty Wood Door Calibrating Wide Belt Sander]

Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Safety Double Wire Security Metal Welded Wire Mesh Fence]

Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Mini LED Flood Light SMD Flood Light 50W, Part I.Ferrite Core for Power Transformer (Ee16e). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Humic Acid Bio-Fertilizer Granule Making Machine]

Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Manufacturer Wallpaper House Decoration Wall Paper.Flexible Magnetic Rubber Sheet Soft Magnetic Tape, 175-184[Elegance Carpet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Whitaker,J.R.(1963),Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. Analytical Chemistry35(12), 1950-1953[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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