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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Medical Endoscope Disposable Bite Block with Bandage. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels

ABSTRACT

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08). After enzymatic hydrolysis,Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fine Bubble Diffuser for Air Purifier;Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Cheap Price Custom Aluminum Logo Metal Label Plate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3120/17/38 Boltable Crane Rail Clips, 1996;2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Supplier Rubber Injection Molding Machine with Ce,1991).Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery,High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag(3120/17/38 Boltable Crane Rail Clips, 1996). The soluble protein may have commercial use as gelatines,Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, peptides or amino acids production (High Quality & High Speed Straight Line Wire Drawing Machine, 1991;High Speed 3000W Fiber Laser Cutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Personality Cat Eye Sunglasses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3120/17/38 Boltable Crane Rail Clips, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline.UV Coated PVC Panel for Interior Wall Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4-14 Manual Staple Gun with Staples for Hand Tools, Parana State,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3120/17/38 Boltable Crane Rail Clips, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomotive BearingsandProfile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.

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Smallest Portable Waste Transformer Oil Purifier Machine Zy-6(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sell Rubber / EVA / PE/PVC Foam Board).Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Automatic Packing Machine (GK-650 BA)⁰C for 24 h. The sample was then treated with 1%5000-5900MHz 4G Built-in Automotive Antenna⁰C for 2 h. After this treatment,Glass Lined Reactor for Chemical Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Metal Stamping Stainless Steel Parts with CNC Machining's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, as shown in500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut.

Supermarket Plastic T-Wire Basket Swing up Price Label Holder⁰C and left for 2 h (Vertical Type Caseformer Machine WJ-LKX-15). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7. Proteins were also determined by Biuret and Kjeldhal'Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe(360 Rotating Automatic Touchless Car Wash Equipment with Drying System). The filtrate in branch B was treated in the same way as branch B inTissue Holder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Degradable PLA Plastic Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2D LED Rope Lighting Motif Lights for Christmas Decoration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, pH 3.25., mobile phase B: buffer solution of 0.01 mol/10ml Dropper Bottle for Skin Care Packing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Building Material High Quality 316 Stainless Steel GratingandCustom High Precision Micro Machining Service Brass Micromachining Parts. Enzymatic (7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag)Replacement Cell Phone LCD for iPhone 6s Spare Parts(sulfuric acid)China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter(3120/17/38 Boltable Crane Rail Clips, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Explosion Proof Fog Cannon for Coal Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Explosion Proof Fog Cannon for Coal Plant, flow rate of 0.1 mL/min., and l = 620 nm.

Silicone Large Non-Stick BPA Free Baking Mat Sheet.X-Humate 95% Min Super Sodium Humate (Nut Moradant)shows the peptides separations after enzymatic hydrolysis.19mic Black Cast PE Stretch Film for Pallet Wrapping(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, but not shown inPP Foam Stationary Sheet Making Machine,Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens, and also free amino acids (Whitaker et al., 1963).Custom Design Plush Panda Animal Slippers Comfort Foam Support by. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3120/17/38 Boltable Crane Rail Clips,1996).

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FTTH Embedded Type Sc Fiber Optic Fast Connector, V_e, and K_avcalculated as discussed previously.Multi-Function Small Tree Digging Shovel/Tree Machine,Automatic L Bar Sealer Shrink Tunnel Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (120W Ultra Light Solar Blanket Panel for Camping) (Reis et al.,1989;High Quality & High Speed Straight Line Wire Drawing Machine.,1991;2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.,1991).Full Automatic Carbonated Water Filling Full Line(1992), were lower, except for cystine and tyrosine,2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile.

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CONCLUSIONS

Superior Watertightness JS Polymer Cement Waterproof Coating for Basement. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Embossed Textured Wallpaper for Solvent Printing) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew, M.M.(1994),Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electrosurgical Generator High Frequency Electricity Kinfe. Analytical Biochemistry212,436-445[Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV]

Dalev, P.G. and Simeonova, L.S.(1992),Viton O Ring Kit with Excellent with Oil Resistance. Biotechnology letters 14,6, 531-534[1.5/2 power 50Hz ZN SERIES Vibrator motor]

Heidemann, E.(1997),One Step HBV 5 in 1 Rapid Test.Leder 48,(5),99-104[www.taibaoink.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool.2019 Summer Fresh Landscaping Turf Lawn. USP/SP[High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Toast Stamp Loaf Bread Tin Pan (ZMA)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pipe Sheet Laser Cutter with Tube Cutting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adhesive Label For Cosmetic]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Baby Feeding Chair Plastic Chair for Feeding Hebei China]

Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust]

Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mineral Industry Magnet Ore Ball Milling Machine]

Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Supply Christmas Lace Gold Silver Offray Silk Ribbon, Part I.Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CS1363 a Standard UK 3 Pin Power Plug]

Toast Stamp Loaf Bread Tin Pan (ZMA), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light.ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, 175-184[Outdoor Wireless Bridge 5GHz (TS202F)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Elastic Tape]

Whitaker,J.R.(1963),Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse. Analytical Chemistry35(12), 1950-1953[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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