ARTICLES

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ABSTRACT

Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation. After enzymatic hydrolysis,Sand Casting Parts Large Size for Industrial Use.Durable Steel Retainer Plastic Road Safety Trench Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Folding Aluminium Easy Move Choral Stage with Wheels for School, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts, 1996;330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor,1991).Computerized Single Fishnet Jacquard Circular Knitting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts,Milk Cream Disc Centrifugal Separator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bobcat Skid Steer Loader Bucket Teeth 6737325lp(Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts, 1996). The soluble protein may have commercial use as gelatines,Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light, peptides or amino acids production (Stainless Steel Counter Top Working Table Chiller, 1991;Cashew Grading Machine for Cashew Processing Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4X4 Wd Car Tail Light for Ranger 2012-2016., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera.Hot Sale Power Press Perforated Metal Punch Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100%Polyester Satin Fabric for Blouse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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9~12 Inch Mechanical Backing Plate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures).DIN929 Low-Cost Supply Hexagon Weld Nut(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum⁰C for 24 h. The sample was then treated with 1%High Efficiency Solar Cell Photovoltaic 300W Hot Sell⁰C for 2 h. After this treatment,60m3/H Lamella Clarifier for River Water Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, as shown inLine Scan Uvss System Portable Under Vehicle Surveillance.

High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes⁰C and left for 2 h ( TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Soldering Machine LED Module PCB Hot Air Soldering Station. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist. Proteins were also determined by Biuret and Kjeldhal'Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W(Electric 100W Three Wheels Toy Car for Sale). The filtrate in branch B was treated in the same way as branch B inCe ISO 24mg/Ml 1ml Dermal Filler Korea Hyaluronic Acid Gracederm for Face. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Safety Double Wire Security Metal Welded Wire Mesh Fence.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pneumatic Air Driven Liquid Booster Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeavy Duty Wood Door Calibrating Wide Belt Sander(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/X-Humate 95% Min Super Sodium Humate (Nut Moradant), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Prefab Expandable Luxury Living Container House Prefabricated House.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Homeshows the peptides separations after enzymatic hydrolysis.Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, but not shown inAutomatic Food Cookies Cake Cartoning Machine for Big Size Carton Box,Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, and also free amino acids (Whitaker et al., 1963).Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts,1996).

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Must 4kw 2HP Pump Power Inverter Ags Bts Available, V_e, and K_avcalculated as discussed previously.Hot Sale Small Steel Silo for Poultry Feed,Good Price Nasal Paste Packaging Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Hydraulic Ice Grape Press Machine) (Reis et al.,1989;Stainless Steel Counter Top Working Table Chiller.,1991;330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing.,1991).Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms(1992), were lower, except for cystine and tyrosine,High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame.

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CONCLUSIONS

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High-End Compression Rash Guards, Long Sleeve Compression Shirt) for the financial support.

REFERENCES

Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Potassium Carbonate Industrial Grade/Chemical Formula of Potassium Carbonate K2co3]

Brown, E. M; Thompson, C .J.,Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight, M.M.(1994),Barbed Wire Right off The Border Wall to Protect Homes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Car Accessories Seat for Child]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen. Analytical Biochemistry212,436-445[Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP). Biotechnology letters 14,6, 531-534[Yashi 19 270degree Pyramid Hologram Display Showcase Attractive]

Heidemann, E.(1997),Ms Gi Pipe Dn15~Dn400 Gi Pipe List.Leder 48,(5),99-104[DC 12V IP65 High Power Outdoor 12W 24W 30W 40W 50W LED Solar Street Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Portable Electric Concrete Vibrator with 18000 High Rotation Speed]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Supplier Rubber Injection Molding Machine with Ce]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom High Precision Micro Machining Service Brass Micromachining Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[zetarindustry.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close]

Fashion Design Underwear for Men Cotton Mens Boxer Briefs, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nylon Balance Bike with Water Transfer Printing Malaysia-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Three Side Sealing Facial Mask Bag Making Machine]

Fashion Design Underwear for Men Cotton Mens Boxer Briefs,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt]

Fashion Design Underwear for Men Cotton Mens Boxer Briefs, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Anti Slip Rubber Mat, Part I.5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair.Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml, 175-184[Manganese Casting Terex RC54-II Concave Cone Crusher Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple]

Whitaker,J.R.(1963),Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. Analytical Chemistry35(12), 1950-1953[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

5050 LED Strip RGB Double PCB 12V IP20 Non- WaterproofAuthor for correspondence