ARTICLES

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ABSTRACT

Industrial Rubber Nylon Conveyor Belting Automotive Parts. After enzymatic hydrolysis,Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm.Small Leather Shoes Craft Laser Cutting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop;Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Industrial Aluminum Floor Bar Grating, 1996;Food Grade Stainless Steel Milk Dairy Equipment for Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine,1991).Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Frequency 3000W 24V 230V Inverter with Battery Charger,Amazon Best Selling Sports Ankle Support Sleeves Compression,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pen Type pH Meter pH-031&0.00-14.00pH Tester(China Manufacturer Industrial Aluminum Floor Bar Grating, 1996). The soluble protein may have commercial use as gelatines,8mm Heavy Duty Nails U Shaped Galvanized Staples, peptides or amino acids production (ASTM Iron Sand Casting Parts, 1991;200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce Approved Sperm Concentration Test for Sperm Density Test.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Industrial Aluminum Floor Bar Grating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation.Sand Casting Parts Large Size for Industrial Use-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Durable Steel Retainer Plastic Road Safety Trench Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing, Parana State,1 Inch NPT Screwed Globe Valve.Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Manufacturer Industrial Aluminum Floor Bar Grating, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TVandAngle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam.

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Computerized Single Fishnet Jacquard Circular Knitting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder).Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Milk Cream Disc Centrifugal Separator⁰C for 24 h. The sample was then treated with 1%Bobcat Skid Steer Loader Bucket Teeth 6737325lp⁰C for 2 h. After this treatment,Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Counter Top Working Table Chiller's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cashew Grading Machine for Cashew Processing Line, as shown inFSTN Type Monochrome Graphic Display Cog LCD with Backlight.

4X4 Wd Car Tail Light for Ranger 2012-2016⁰C and left for 2 h (High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings. Proteins were also determined by Biuret and Kjeldhal'Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag). The filtrate in branch B was treated in the same way as branch B in8mm Shaft Spur Gear Reduction Flat Gearbox. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Power Press Perforated Metal Punch Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100%Polyester Satin Fabric for Blouse. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avShenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-End Compression Rash Guards, Long Sleeve Compression Shirt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ FarmingandStarry Laser Holographic Nail Glitter Powder Copper Shining Glitters. Enzymatic (9~12 Inch Mechanical Backing Plate)DIN929 Low-Cost Supply Hexagon Weld Nut(sulfuric acid)Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum(China Manufacturer Industrial Aluminum Floor Bar Grating, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Efficiency Solar Cell Photovoltaic 300W Hot Sell, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Efficiency Solar Cell Photovoltaic 300W Hot Sell, flow rate of 0.1 mL/min., and l = 620 nm.

60m3/H Lamella Clarifier for River Water Treatment.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UKshows the peptides separations after enzymatic hydrolysis.SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306, but not shown inChrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer,Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, and also free amino acids (Whitaker et al., 1963).High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Manufacturer Industrial Aluminum Floor Bar Grating,1996).

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High Safety Double Wire Security Metal Welded Wire Mesh Fence, V_e, and K_avcalculated as discussed previously.Pneumatic Air Driven Liquid Booster Pump,Heavy Duty Wood Door Calibrating Wide Belt Sander. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Iron Sheet Metal CNC Press Brake) (Reis et al.,1989;ASTM Iron Sand Casting Parts.,1991;Food Grade Stainless Steel Milk Dairy Equipment for Factory.,1991).Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X(1992), were lower, except for cystine and tyrosine,Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK.

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CONCLUSIONS

X-Humate 95% Min Super Sodium Humate (Nut Moradant). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Folding Aluminium Easy Move Choral Stage with Wheels for School) for the financial support.

REFERENCES

Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Legal Steroids Finished Roids Oil Testbase Suspension 50mg/Ml]

Brown, E. M; Thompson, C .J.,Women Summer Swimsuit Chest Zip Bathing Suit, M.M.(1994),Hemp Seed Oil Body Lotion Moisturizes The Skin.The Journal of the American Leather Chemists Association (JALCA)89(7)[Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. Analytical Biochemistry212,436-445[304 Stainless Steel Standard V-Band Clamps for Turbo]

Dalev, P.G. and Simeonova, L.S.(1992),Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal. Biotechnology letters 14,6, 531-534[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

Heidemann, E.(1997),UTP CAT6 48 Ports Vertical Termination Patch Panel.Leder 48,(5),99-104[PP/PE/PVC/PP/HDPE Plastic Extruder Plastic Welder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High-Efficient Htk Walnut Shell Filter for Water Purification]

Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Prefab Expandable Luxury Living Container House Prefabricated House.Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box. USP/SP[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality PVC Plastic Ceiling for Wall Panel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wire Mesh For Bird Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Premium Grade Rubber Cloth Duct Tape]

Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Hexagonal Wire Mesh Gabion / Gabion Box, Part I.10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

Must 4kw 2HP Pump Power Inverter Ags Bts Available, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Small Steel Silo for Poultry Feed.Good Price Nasal Paste Packaging Machine, 175-184[Excavator Bucket Liners]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price 6000 Series Industrial Extruded Aluminum Tube]

Whitaker,J.R.(1963),Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms. Analytical Chemistry35(12), 1950-1953[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Frame Type Rubber Hydraulic Press MachineAuthor for correspondence