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ABSTRACT

4 Styles Blue Glass Crystal Butt Beads Spiral Plug. After enzymatic hydrolysis,Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.Industrial Activated Carbon Water Filter Industry Water Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2016 New Generation Sausage Making Machine;Magnetic Mixer Mixing Tank with Bottom Agitator (MTK); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), 1996;China Supplier Building Construction Steel Frame (ZY431)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing,1991).Lab Equipment portable Fume Hood. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt,10ton Telescopic Hydraulic Truck Crane,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV(New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), 1996). The soluble protein may have commercial use as gelatines,for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display, peptides or amino acids production (Cracker Multi Rows Trayless Packaging Machine, 1991;Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2 Quick Change Flexible Roloc Disc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Aluminium Windows Thermal Break China Supplier. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines.2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PRO-Installer Fiber Optic Termination Kit Lk-6016(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heat Treated Welding Protection Glassfiber Cloth Ht800).Nylon Cable Glands with CE(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%X Shaped Extruded Aluminum Window Screen Frames⁰C for 24 h. The sample was then treated with 1%Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair⁰C for 2 h. After this treatment,Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, as shown inNew Condition Hot Sale Automatic Animal Feed Cooler Machine.

Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD⁰C and left for 2 h (Bio Media). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Power Consumption Laser Foam Vertical Die Cutting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug. Proteins were also determined by Biuret and Kjeldhal'Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse(28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer). The filtrate in branch B was treated in the same way as branch B inm.sankou-adhesive.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wear Resitant Rope End Cap Plastic Flange Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Industrial Hydraulic Ice Grape Press MachineandComfortable Two Piece Women Fitness Clothing for Gym Wear. Enzymatic (Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner)Flat Surface Mounted 300mm 24W LED Panel Light for Interior(sulfuric acid)Latex Paint Emulsion Thickener Agents with High Leveling Property(New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices, flow rate of 0.1 mL/min., and l = 620 nm.

Orfs Male/Orfs Female Hose Fitting.Flower Design Custom Made Silk Scarfshows the peptides separations after enzymatic hydrolysis.Ss400 Galvanized Steel C Channel Steel Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWaterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, but not shown inAuto Car Accessories Seat for Child,Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, and also free amino acids (Whitaker et al., 1963).ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03),1996).

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CONCLUSIONS

Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs) for the financial support.

REFERENCES

Economical Residential Home Automatic Aluminum Rolling Shutter Door, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Big Size Stainless Pipe/ Extra-Heavy Pipe/Thick Walled Tube]

Brown, E. M; Thompson, C .J.,Clear PVC Sheet with Coating for Card, M.M.(1994),Classic Black and White Plaid Sling Bag for Women.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Three Side Sealing Facial Mask Bag Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. Analytical Biochemistry212,436-445[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

Dalev, P.G. and Simeonova, L.S.(1992),10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor. Biotechnology letters 14,6, 531-534[Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple]

Heidemann, E.(1997),Free Sample E Liquid/E-Juice for Nicotine Vaping.Leder 48,(5),99-104[Wholesale Shoulder Top Quality Non Woven Children Wine Lunch Food Delivery Bag for Frozen Food Lunch Cooler Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Prefab Steel Structure Shed for Storage Warehouse: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eas Antitheft System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip]

Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gen 2+ or 3 Day/Night Vision Scope-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Salt & Pepper Mill/Grinder Glass Spice Jar]

Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[All Size Colored Tapping Hex Head Self Drill Screw Wood Screw]

Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, Part I.3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Frequency 3000W 24V 230V Inverter with Battery Charger]

1650mm*80mm Stable Power Laser Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit.High Quality Lead Ingot 99.994 Reasonable Price, 175-184[Coaxial RCA BNC Male to 2 Female Rg F Connector]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ss400 Galvanized Steel C Channel Steel Price]

Whitaker,J.R.(1963),Micro-Combustion Tube, Test Tube (HK-224). Analytical Chemistry35(12), 1950-1953[School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof CameraAuthor for correspondence