ARTICLES

Farm & Industry Used Storage Grain Silo

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-St1000 Fire Resistant Steel Cord Rubber Belt. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Four Color Long Sleeve Yoga Wear

ABSTRACT

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. After enzymatic hydrolysis,China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes.Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck;XL-21 Type OEM Motive Electrical Switchgear Power Cabinet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (White HPL 600*600mm Coffee Table, 1996;Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Price 6000 Series Industrial Extruded Aluminum Tube,1991).WPC Decking Profile Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile,CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Party Mask LED Fiber Dance Princess Feather Mask(White HPL 600*600mm Coffee Table, 1996). The soluble protein may have commercial use as gelatines,80GSM C2s Coated Glossy/Matt Art Paper for Printing, peptides or amino acids production (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, 1991;Reusable Silicone Straight Straws, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Easy Foldable Kids Walking Chair Toys Baby Walker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yellow Hazardous Spill Response Chemical Spill Kits.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Anhui Hydraulic Sheet Metal Bending Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (White HPL 600*600mm Coffee Table, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304 Stainless Steel Standard V-Band Clamps for Turbo.Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden LightAlcalase2.5 L DX,Round Casting Iron Trench Cover Manhole Cover Frame, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Eco-Friendly Aluminum Mechanical Part, CNC Machining(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, Parana State,Four Color Long Sleeve Yoga Wear.Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (White HPL 600*600mm Coffee Table, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOutdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED FloodlightandStainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle.

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Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys).Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper⁰C for 24 h. The sample was then treated with 1%Ductile Iron Flanged Swing Check Valve⁰C for 2 h. After this treatment,Ozonated Water for Vegetables and Fruits Washing Ozone Generator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Day and Night Zebra Blind Fabric Z1602, as shown inCE Certificated Wheel Loader (HQ912) with Agricultural Tire.

40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock⁰C and left for 2 h (High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Impact Sound Isolation Wall Clip. Proteins were also determined by Biuret and Kjeldhal'Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(Factory Price 6mm Stainless Steel Wire Rope 7*7). The filtrate in branch B was treated in the same way as branch B inStainless Steel Perforated Sheets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,German Technology Automatic Stacking System in Red Brick Automatic Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Top Quality Galvanized Iron Wire as Binding Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av13.56 MHz Fudan FM11RF08 Chip Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal TubeandSquare Premium Glass Perfume Bottle 100ml. Enzymatic (Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds)Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed(sulfuric acid)Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep(White HPL 600*600mm Coffee Table, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Inflatable PVC Flocking Health Bed.4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Leggingshows the peptides separations after enzymatic hydrolysis.Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlass Food Jar & Plastic Lid, but not shown inNPK 15 15 15 Compound Fertilizer at a Hot Selling,Cast Iron Butterfly Valve with Handles, and also free amino acids (Whitaker et al., 1963).Furniture Grade E1 Glue Plywood with Competitive Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (White HPL 600*600mm Coffee Table,1996).

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Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machineand14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cupshow the gel chromatographic separation of soluble protein with Sephadex G-28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.Underground Garden 6W High Power Outdoor LED Lawn Light, and another peak at 45 mL not shown in0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, for peptides lower than 3000 Da.Stamping Metal Parts Manufacturer Sheet Parts Fabrication(Condell et al.,1993) .

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Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, V_e, and K_avcalculated as discussed previously.3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY,Hexagonal Netting/ Gabion Box/ Gabion Basket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Vitamin B1 HCl 98% Feed Grade) (Reis et al.,1989;Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter.,1991;Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale.,1991).Different Thickness High Quality Aluminum Foil for Glass Bottle Lid(1992), were lower, except for cystine and tyrosine,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla.

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CONCLUSIONS

Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane) for the financial support.

REFERENCES

Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale! ! PP Fibre Micro Polypropylene Concrete Fiber for Engineering]

Brown, E. M; Thompson, C .J.,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, M.M.(1994),Stainless Steel Ozone Disinfection Machine Ozone Generator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. Analytical Biochemistry212,436-445[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Profile with Net Making Machine Used for Window Sealing Profile. Biotechnology letters 14,6, 531-534[Aluminum Low Profile Adjustable Bracket]

Heidemann, E.(1997),Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch.Leder 48,(5),99-104[94% Mini Calcium Chloride]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump]

FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bobby Flay Websites Restaurant Martha Stewart Designer Tray.Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. USP/SP[22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pen Type pH Meter pH-031&0.00-14.00pH Tester]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4x6 Folding Tent]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stamping Metal Parts Manufacturer Sheet Parts Fabrication]

Diameter 2.68m Paragraphed Autoclave for AAC Bricks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

Diameter 2.68m Paragraphed Autoclave for AAC Bricks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight]

Diameter 2.68m Paragraphed Autoclave for AAC Bricks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Travelling Weekender Mens Canvas Duffle Gym Sports Bag, Part I.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Selling Household Living Room Mat Chenille Carpets.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 175-184[zetarindustry.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China]

Whitaker,J.R.(1963),Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. Analytical Chemistry35(12), 1950-1953[Powder Coating Polyester Resin with Factory Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60Author for correspondence