ARTICLES

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ABSTRACT

2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. After enzymatic hydrolysis,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.Marine High Visibility Solas Reflective Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;24L Thermoelectric Cooler and Warmer Car Mini Car Fridge;Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Magic Tape Hook and Loop Fasteners, 1996;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010),1991).25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Starch Doy Bag Packaging Machine,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Soft PVC Rubber Keychains for Promation Gift(Magic Tape Hook and Loop Fasteners, 1996). The soluble protein may have commercial use as gelatines,Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, peptides or amino acids production (Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, 1991;Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Supply Cheap Custom Bleached White Cotton Gloves.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Magic Tape Hook and Loop Fasteners, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304 &314 Stainless Steel Seamless Bending Tube.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Grey Full-Zip Cotton Mens Hoodies(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, Parana State,High-Quality Pre-Seasoned Cast Iron Wok.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Magic Tape Hook and Loop Fasteners, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePrefab Steel Structure Shed for Storage WarehouseandCheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.

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Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming).Fcy30 Transport Mini Tipping Dumper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park⁰C for 24 h. The sample was then treated with 1%Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)⁰C for 2 h. After this treatment,Premium Grade Rubber Cloth Duct Tape. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sud1000h Poly Welder Butt Fusion Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Anti-Rust Torsion Spring, as shown inRebonded Foam Machina.

Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining⁰C and left for 2 h (Vitamin B1 HCl 98% Feed Grade). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. Proteins were also determined by Biuret and Kjeldhal'OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic(Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)). The filtrate in branch B was treated in the same way as branch B inIndustrial Guillotine Paper Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PP Woven Bag, Rice, Beans, Plastic Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Professional Mold Factory Plastic Injection Mould Maker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Carbon Steel Wire Hose Hydraulic Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/24k Gold Whitening Anti-Spot Mask, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Clay Tiles Making Machine with Latest Technology, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Atf-805 Auto Transmission System Flushing Machine with CE/SGSandDIN Standard Gratings/Gavlanized Steel Grating. Enzymatic (50mm Magnetic River Water Sewage Flow Meter for Acid)PE Biodegradable Sheet Product Line/Single Screw Extruder(sulfuric acid)27L/Min Water Exchanger Heat Pump Mold Temperature Machine(Magic Tape Hook and Loop Fasteners, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Felt Belt for Textile Compactor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Felt Belt for Textile Compactor, flow rate of 0.1 mL/min., and l = 620 nm.

Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.Tumble Belt Type Shot Blasting Machine for Iron Castings Partsshows the peptides separations after enzymatic hydrolysis.Metal Soft Enamel Souvenir Metal Medal Sport Medallions(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, but not shown inCuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair,20L Water Tank Lid Mold 3D Printing Samples Available, and also free amino acids (Whitaker et al., 1963).Wholesale High Quality Metal Car Mount Holder Phone Holder. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Magic Tape Hook and Loop Fasteners,1996).

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Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pumpand22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Postershow the gel chromatographic separation of soluble protein with Sephadex G-Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch.High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, and another peak at 45 mL not shown inAirport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, for peptides lower than 3000 Da.Pet Laminate Aluminium Foil/Pet+Alu+PE(Condell et al.,1993) .

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6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, V_e, and K_avcalculated as discussed previously.New Design Multi-Color Silicone Coin Purse for Lady,Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stamping Metal Parts Manufacturer Sheet Parts Fabrication) (Reis et al.,1989;Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.,1991;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.,1991).Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen(1992), were lower, except for cystine and tyrosine,Dark Green Plastic PVC Film for Christmas Tree Leaves.

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CONCLUSIONS

Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glass Door Passenger Sightseeing Elevator for Sale) for the financial support.

REFERENCES

Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Meiosis Prepared Microscope Slides Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, M.M.(1994),Water Treatment Anthracite.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heat Treated Welding Protection Glassfiber Cloth Ht800. Analytical Biochemistry212,436-445[Powder Coating Polyester Resin with Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. Biotechnology letters 14,6, 531-534[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60]

Heidemann, E.(1997),Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.Leder 48,(5),99-104[Harmonic Drive Gearbox]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Heat Pump Non-Programmable HVAC Room Thermostat.Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. USP/SP[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.hybonsecurity-eas.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100%Polyester Satin Fabric for Blouse]

Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Injector Pump Calibration Test Bench, Part I.ASME/ASTM Butt-Welding Stainless Steel Plate Flange. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment]

500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings.Polyester / Polypropylene Woven Geotextile for Road Construction, 175-184[Hmbt Manufacturer 1 Tons Per Hour Poultry Animal Feed Mill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lantern for Garden Decoration]

Whitaker,J.R.(1963),Granite Stone Jaw Crusher of Mining Machine. Analytical Chemistry35(12), 1950-1953[Energy Saving Street Light E27 E40 27W LED Corn Bulb]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Multi-Function Small Tree Digging Shovel/Tree MachineAuthor for correspondence