ARTICLES

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ABSTRACT

Powerful Anti-Stress, Strong Flexibility Spoke Wire. After enzymatic hydrolysis,Modern American Style Beech Solid Wood Kitchen Cabinet.Hero Brand Polypropylene Bags Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Machining Metal/Aluminum/Brass Auto Parts;Surprise! Range Hood Free! ! ! Juice Cart; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chicken Layer Cage Poultry Cage Chicken Cages, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (316L Cold Rolled Stainless Steel Coil 316L Inox, 1996;Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fully Automatic C Z Purlin Cold Roll Forming Machinery,1991).Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes,En1092-1 05 Pn16 Blind Flat Face Flange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag(316L Cold Rolled Stainless Steel Coil 316L Inox, 1996). The soluble protein may have commercial use as gelatines,Peristaltic Pump with Connecting The High Speed Tubular Separator, peptides or amino acids production (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, 1991;LED Bulb Light with Remote Control RGB Christmas Bulb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Home Decoration Kids Toy Promotion Eyeglass Plastic Mould., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Price Gi/PPGI Roofing Sheet in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (316L Cold Rolled Stainless Steel Coil 316L Inox, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed.Multipurpose Wireless PA Amplifier Lectern PA Speaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell).PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture⁰C for 24 h. The sample was then treated with 1%Imirootree 250ml Glass Jar with Lids for Cosmetic Cream⁰C for 2 h. After this treatment,Double Wine Carrier Bag Gift Box Tote. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, as shown inDouble-Offset Double Flanged Stainless Steel Butterfly Valve.

Flexible PVC Granules for Cable, Wire and Insulation⁰C and left for 2 h (7inch Industrial Tablet Computer Naked Resistance Touch Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. Proteins were also determined by Biuret and Kjeldhal'Brass Pump Spare Parts for Circulating Pump(Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)). The filtrate in branch B was treated in the same way as branch B inDisposable Vinyl Gloves. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bending Sheet Metal Custom-Made Aluminum Bracket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Shacman F3000 6X4 Truck Trailer Head, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.24 Fiber MPO MTP Breakout Cassette with MPO to LC Cableshows the peptides separations after enzymatic hydrolysis.Activated Carbon Strong Effect Peeling off Face Mask Mud(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWatch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, but not shown inMining Slurry Dewatering Rotary Precision Disc Ceramic Filter,Type Price Prefabricated Roof Tile in Sudan, and also free amino acids (Whitaker et al., 1963).Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (316L Cold Rolled Stainless Steel Coil 316L Inox,1996).

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CONCLUSIONS

Full Color Child Book Printing Service. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12ml Round Shape Empty Clear Nail Polish Glass Bottle) for the financial support.

REFERENCES

1.5 Inch High Pressure Petrol/Gasoline Water Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lip Balm Tubes]

Brown, E. M; Thompson, C .J.,Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, M.M.(1994),Equal and Unequal High Quality Hot Rolled Steel Angle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Power Press Perforated Metal Punch Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Halal Chicken Slaughter Machine for Sale. Analytical Biochemistry212,436-445[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop. Biotechnology letters 14,6, 531-534[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Heidemann, E.(1997),2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr.Leder 48,(5),99-104[Dn200 Manual Stainless Steel Wafer Butterfly Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Quality Tomato Sauce Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[from China to India Chennai(MAA)Airport Air Logistics Freight Shipping Agent reliable Forwarder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder]

High Quality Xanthan Gum for Personal Care and Cosmetic Applications, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manual Vertical Car Key Cutting Machine for Locksmith-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+]

High Quality Xanthan Gum for Personal Care and Cosmetic Applications,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Thermostat Electric Heating Element for Solar Water Heaters]

High Quality Xanthan Gum for Personal Care and Cosmetic Applications, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape, Part I.Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacturer Custom Precision Aluminum Die Casting LED Light Housing]

Transparent Color Stretch Wrap Film Manual Operate Wrap Film, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price Welding Electrode (e7018/e7018-1).Ep/Nn/EPP Industrial Rubber Conveyor Belt, 175-184[Fine 8 Hydraulic Breaker Seal Kit for Fine 8 Hydraulic Hammer Seal Kit Fine 8 Breaker Seal Kit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)]

Whitaker,J.R.(1963),Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing. Analytical Chemistry35(12), 1950-1953[Degradable PLA Plastic Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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