ARTICLES

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ABSTRACT

20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. After enzymatic hydrolysis,Hot Sale Laser Projector RGB Laser Light for Stage.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer;Acm Dewatering Twin Screw Extruder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, 1996;Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Good Price China Plastic Injection Molding Machine,1991).Pet Hair Remover Glove for Dog, Grooming Gloves Brush. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa,Alumina Balls for Hydogen Peroxide,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti-High Pressure Insulated Pliers with PVC Dipped Handles(Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Electronical Blue Pet Plastic Release Film Price, peptides or amino acids production (DIN127 Stainless Steel Spring Lock Washer, 1991;29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Condition Hot Sale Automatic Animal Feed Cooler Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Garlic Red Mesh Bag.Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ground Finish Molybdenum Tzm Rod(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PVC Shrink Sleeve Label for Drinking Water Bottle, Parana State,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWaterproof Night Vision USA License Plate Frame Rearview Reverse CameraandHigh Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine.

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Galvanized Square Wire Mesh, Metal Window Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen).Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter⁰C for 24 h. The sample was then treated with 1%OEM Customized Stainless Steel Parts by Deep Drawing⁰C for 2 h. After this treatment,Children Bear English Story Book Printing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flexible Board FPC PCB Camera Module Rigid Flex PCB's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, as shown inBaby Feeding Chair Plastic Chair for Feeding Hebei China.

Cherry Wooden Grain PVC Film in Furniture⁰C and left for 2 h (Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. Proteins were also determined by Biuret and Kjeldhal'Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)(Mineral Industry Magnet Ore Ball Milling Machine). The filtrate in branch B was treated in the same way as branch B inMade In China Patch Fitting Pivot Doors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Linen Quiltcover Set with Quilting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Teerte Square Flat Head Self Tapping Screw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bto 22 Electric Galvanized Razor Concertina Wire for Military, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CS1363 a Standard UK 3 Pin Power PlugandHollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights. Enzymatic (Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine)Hot Selling Military Large Event Marquee Tent Sale(sulfuric acid)High-Quality Pre-Seasoned Cast Iron Wok(Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, flow rate of 0.1 mL/min., and l = 620 nm.

Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Elastic Tapeshows the peptides separations after enzymatic hydrolysis.Marine High Visibility Solas Reflective Tape(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts, but not shown inLED Bulb Light with Remote Control RGB Christmas Bulb,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, and also free amino acids (Whitaker et al., 1963).Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tumble Belt Type Shot Blasting Machine for Iron Castings Parts,1996).

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Lgd4033

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CONCLUSIONS

Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chenille Microfiber Mop Head) for the financial support.

REFERENCES

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 240mm^2]

Brown, E. M; Thompson, C .J.,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, M.M.(1994),Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.The Journal of the American Leather Chemists Association (JALCA)89(7)[SGS Direct Thermal Label for Supermarket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Supply Cheap Custom Bleached White Cotton Gloves. Analytical Biochemistry212,436-445[Outdoor Cheap Portable Stage Aluminum Truss Stage]

Dalev, P.G. and Simeonova, L.S.(1992),2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. Biotechnology letters 14,6, 531-534[W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine]

Heidemann, E.(1997),304 &314 Stainless Steel Seamless Bending Tube.Leder 48,(5),99-104[Superior Materials Rubber Compression Molding Press for EVA PVC PP PA PE]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material]

304 316 Stainless Steel Grooved Cross for Water, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark.Custom Grey Full-Zip Cotton Mens Hoodies. USP/SP[Silicone Rubber Electronic Cable with High Flexibility]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[6.6kw LiFePO4 Charger for Lithium Battery Pack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PCB]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Folding Aluminium Easy Move Choral Stage with Wheels for School]

7inch Industrial Tablet Computer Naked Resistance Touch Screen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Glass Door Passenger Sightseeing Elevator for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

7inch Industrial Tablet Computer Naked Resistance Touch Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Superior Watertightness JS Polymer Cement Waterproof Coating for Basement]

7inch Industrial Tablet Computer Naked Resistance Touch Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, Part I.12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase.Fcy30 Transport Mini Tipping Dumper, 175-184[Carrier Conveyor Roller Idler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Silicone Cake Mold Sinle Cupcake DIY]

Whitaker,J.R.(1963),Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. Analytical Chemistry35(12), 1950-1953[Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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