ARTICLES

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ABSTRACT

Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. After enzymatic hydrolysis,Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.BS 5163 Ductile Iron Rising Stem Gate Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Aluminum Sheet Press Brake, 1996;ID25mm Od86mm Standard Through Hole Slip Ring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding,1991).Bio Media. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine,Platform Trolley with 150kg Capacity, 4 Castor Wheel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine(CNC Aluminum Sheet Press Brake, 1996). The soluble protein may have commercial use as gelatines,LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, peptides or amino acids production (All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 1991;Pre-Engineered Cage Broiler Poultry Farm Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Alumina Ball with High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Textile Fabric for Outdoor Sports Waterproof Backpack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-White 4*8FT 18mm PVC Foam Board for Cabinets Use. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Aluminum Sheet Press Brake, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Powerful Anti-Stress, Strong Flexibility Spoke Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Chicken Layer Cage Poultry Cage Chicken Cages, Parana State,Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.316L Cold Rolled Stainless Steel Coil 316L Inox, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Aluminum Sheet Press Brake, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePromotional Jewelry Gift Paper Box with Ribbon and Inner TrayandBS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized.

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Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( LED Bayomet Vehicle Lamps).Fully Automatic C Z Purlin Cold Roll Forming Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant⁰C for 24 h. The sample was then treated with 1%Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes⁰C for 2 h. After this treatment,En1092-1 05 Pn16 Blind Flat Face Flange. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Peristaltic Pump with Connecting The High Speed Tubular Separator, as shown inPhillip Countersunk Head Screw Hot DIP Galvanized.

High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding⁰C and left for 2 h (Automatic CNC Glass Cutting Machine Glass Cutting Table). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED Bulb Light with Remote Control RGB Christmas Bulb. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display(Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook). The filtrate in branch B was treated in the same way as branch B inHot Sell Reflective Flex Glossy. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Price Gi/PPGI Roofing Sheet in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMultipurpose Wireless PA Amplifier Lectern PA Speaker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12ml Round Shape Empty Clear Nail Polish Glass Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Lightshows the peptides separations after enzymatic hydrolysis.Imirootree 250ml Glass Jar with Lids for Cosmetic Cream(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in18X16mesh Fiberglass Window Screen Insect Screen, but not shown inCross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap,Double Wine Carrier Bag Gift Box Tote, and also free amino acids (Whitaker et al., 1963).(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Aluminum Sheet Press Brake,1996).

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Pipe Conveyor BeltandHydraulic Orbital Valve Power Steeringshow the gel chromatographic separation of soluble protein with Sephadex G-Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord.Flexible PVC Granules for Cable, Wire and Insulation, and another peak at 45 mL not shown inGyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, for peptides lower than 3000 Da.7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security(Condell et al.,1993) .

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Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, V_e, and K_avcalculated as discussed previously.Brass Pump Spare Parts for Circulating Pump,Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hanging Necklace Fan USB Rechargeable Battery Operated Fan) (Reis et al.,1989;All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.,1991;ID25mm Od86mm Standard Through Hole Slip Ring.,1991).Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi(1992), were lower, except for cystine and tyrosine,Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011.

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CONCLUSIONS

Lantern for Garden Decoration. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Machining Metal/Aluminum/Brass Auto Parts) for the financial support.

REFERENCES

Bending Sheet Metal Custom-Made Aluminum Bracket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Girls Plain Cotton Dress High Quality Supplier]

Brown, E. M; Thompson, C .J.,New Shacman F3000 6X4 Truck Trailer Head, M.M.(1994),Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. Analytical Biochemistry212,436-445[Hot Sale Electric LED light Aroma Diffuser]

Dalev, P.G. and Simeonova, L.S.(1992),Cylindrical Shape Beer Glass Bottle for Wine Storage. Biotechnology letters 14,6, 531-534[Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest]

Heidemann, E.(1997),Plastic Injection for OEM Product.Leder 48,(5),99-104[Supermarket Display Sliding Glass Door Chest Island Freezer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer]

CNC Fiber Laser Cutting Machine for Sheet and Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. USP/SP[ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Spare Parts 12629037 Ignition Coil for Cadillac]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Backup Chargers 20000]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[St1000 Fire Resistant Steel Cord Rubber Belt]

Activated Carbon Strong Effect Peeling off Face Mask Mud, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Type Price Prefabricated Roof Tile in Sudan-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor]

Activated Carbon Strong Effect Peeling off Face Mask Mud,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment]

Activated Carbon Strong Effect Peeling off Face Mask Mud, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, Part I.Factory Supply 100% Natural Epimedium Extract for Male Health. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket]

Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug.Top10 1080P H. 264 P2p CCTV WiFi IP Camera, 175-184[Assembled]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Color Coated Aluminum Coils]

Whitaker,J.R.(1963),Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture. Analytical Chemistry35(12), 1950-1953[High Performance Multifunctional Crawler Mini Skid Steer Loader]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Custom CNC Milled Precision Spare Part CNC Pen Automobile PartsAuthor for correspondence