ARTICLES

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ABSTRACT

High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine. After enzymatic hydrolysis,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer.Chinese Jbcz Handy Operation Threading Machine and Coupler. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price Printing Doy Pack Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, 1996;New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price,1991).High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Engine Parts Rubber High Quality Timing Belt 111ru25,Wholesale Interior Metal 3D Bee Garden Ornament Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket(Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, 1996). The soluble protein may have commercial use as gelatines,Disposable Non-Woven Dry Gloves Wipes Making Machine, peptides or amino acids production (ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, 1991;Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bottom Pushing for Fire Extinguisher Hot Spinning Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fish Scales Texture Fashion Leather for Shoe (S312100GH).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diamond Segments 2000mm Diameter Granite Cutting Blade. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Produce N2o for Medical Gas Equipments.Hot Selling Furring Channel Fittings Suspended Ceiling Accessories-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:90m3 Per Hour Mobile Concrete Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Speed Automatic Packing Machine (GK-650 BA)).100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)⁰C for 24 h. The sample was then treated with 1%Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle⁰C for 2 h. After this treatment,Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)125kHz RFID Hotel Key Card with T5577 Chip, as shown inElectric Paint Sprayer Pump.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Thermostat Electric Heating Element for Solar Water Heaters(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heat Shrink Automatic Flow Wrapping Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Shanghai China Professional Plastic Injection Moulding Plastic Mold MakerandGuided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. Enzymatic (Ductile Iron Standard Brackets Pipe Hanger)Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers(sulfuric acid)ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper(Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Sheet Metal Bending Roll Forming Machine.Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Saleshows the peptides separations after enzymatic hydrolysis.Different Size Glass Test Tubes, High Quality Laboratory Test Tubes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels, but not shown inWholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, and also free amino acids (Whitaker et al., 1963).Frame Type Rubber Hydraulic Press Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment,1996).

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CONCLUSIONS

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (All Weather Rattan Handwoven Swing Outdoor Home) for the financial support.

REFERENCES

Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Shrinking Machine Packing Package Packaging Machinery Factory]

Brown, E. M; Thompson, C .J.,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, M.M.(1994),New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels.The Journal of the American Leather Chemists Association (JALCA)89(7)[Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck. Analytical Biochemistry212,436-445[304 &314 Stainless Steel Seamless Bending Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. Biotechnology letters 14,6, 531-534[Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient]

Heidemann, E.(1997),ADSS Newest Ultra Therapy Skin Tightening 3D Hifu.Leder 48,(5),99-104[Class 1 Long Rubber Gloves to Prevent Electric Shock]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car]

Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. USP/SP[High Quality & High Speed Straight Line Wire Drawing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[125kHz RFID Hotel Key Card with T5577 Chip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Ceramic Chrome Hybrid Bearing Oil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Black Galvanized Head Stainless Steel Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adjustable Ce ISO Approved 6-15ton Hydraulic Mobile Container Dock Ramp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard]

Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Spine Shape Landscaping Artificial Turf Grass]

Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sanjing PVC Lining Layflat Fire Hose]

Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Digger Arm Small Wheel Loader (HQ908) with CE, Part I.15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Based Emulsion Paint Production Line.Cnm Single Strand Anchor Coupler, 175-184[5-Ton/24 Hr. Ice Maker Block Ice Making Machine with Cooling Tower]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Classic Black and White Plaid Sling Bag for Women]

Whitaker,J.R.(1963),Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. Analytical Chemistry35(12), 1950-1953[ Black Diamond]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Speed 3000W Fiber Laser CutterAuthor for correspondence