ARTICLES

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ABSTRACT

Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44). After enzymatic hydrolysis,Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.Linen Quiltcover Set with Quilting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Teerte Square Flat Head Self Tapping Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, 1996;Bto 22 Electric Galvanized Razor Concertina Wire for Military., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights,1991).Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Selling Military Large Event Marquee Tent Sale,High-Quality Pre-Seasoned Cast Iron Wok,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle(Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, 1996). The soluble protein may have commercial use as gelatines,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, peptides or amino acids production (Marine High Visibility Solas Reflective Tape, 1991;24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Top Quality Waterproof IP66 Aluminum Die Cast Junction Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Magic Tape Hook and Loop Fasteners. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Blasting Cleaning Shot Peeling Machine Tumble Belt Type).Factory Supply Cheap Custom Bleached White Cotton Gloves(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle⁰C for 24 h. The sample was then treated with 1%304 &314 Stainless Steel Seamless Bending Tube⁰C for 2 h. After this treatment,Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Grey Full-Zip Cotton Mens Hoodies's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, as shown in3D Embossed Iron on Label Clothing Heat Transfer Label.

7inch Industrial Tablet Computer Naked Resistance Touch Screen⁰C and left for 2 h (LED Solar Powered Traffic Blinking Emergency Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Glass Door Passenger Sightseeing Elevator for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. Proteins were also determined by Biuret and Kjeldhal'12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor(Black Steel ASTM A192 Seamless Pipe Sch120). The filtrate in branch B was treated in the same way as branch B inm.orist-fashion.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFcy30 Transport Mini Tipping Dumper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Premium Grade Rubber Cloth Duct Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sud1000h Poly Welder Butt Fusion Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PP Woven Bag, Rice, Beans, Plastic Bag.Korean Style PE Foam 3D Wallpapersshows the peptides separations after enzymatic hydrolysis.China Professional Mold Factory Plastic Injection Mould Maker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in50m Depth Long Range Metal Detector with Reliable Quality, but not shown inCustomized Anti-Rust Torsion Spring,Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, and also free amino acids (Whitaker et al., 1963).Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard,1996).

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Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical EquipmentandGood Price Welding Electrode (e7018/e7018-1)show the gel chromatographic separation of soluble protein with Sephadex G-Industrial Carbon Steel Wire Hose Hydraulic Pipe.24k Gold Whitening Anti-Spot Mask, and another peak at 45 mL not shown in Printed Socks, for peptides lower than 3000 Da.Clay Tiles Making Machine with Latest Technology(Condell et al.,1993) .

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DIN Standard Gratings/Gavlanized Steel Grating, V_e, and K_avcalculated as discussed previously.50mm Magnetic River Water Sewage Flow Meter for Acid,PE Biodegradable Sheet Product Line/Single Screw Extruder. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace) (Reis et al.,1989;Marine High Visibility Solas Reflective Tape.,1991;Bto 22 Electric Galvanized Razor Concertina Wire for Military.,1991).27L/Min Water Exchanger Heat Pump Mold Temperature Machine(1992), were lower, except for cystine and tyrosine,Felt Belt for Textile Compactor.

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Soft PVC Rubber Keychains for Promation Gift) for the financial support.

REFERENCES

Metal Soft Enamel Souvenir Metal Medal Sport Medallions, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Outdoor Nylon Parachute Ripstop Heavy Duty Hammock Camping]

Brown, E. M; Thompson, C .J.,20L Water Tank Lid Mold 3D Printing Samples Available, M.M.(1994),Wholesale High Quality Metal Car Mount Holder Phone Holder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yellow Hazardous Spill Response Chemical Spill Kits]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. Analytical Biochemistry212,436-445[Easy Operation Low Investment Cassava Silo]

Dalev, P.G. and Simeonova, L.S.(1992),High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. Biotechnology letters 14,6, 531-534[High Quality Sheet Metal Bending Roll Forming Machine]

Heidemann, E.(1997),Pet Laminate Aluminium Foil/Pet+Alu+PE.Leder 48,(5),99-104[Canbus License Light For Aaudi]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

Cherry Wooden Grain PVC Film in Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.New Design Multi-Color Silicone Coin Purse for Lady. USP/SP[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Color Child Book Printing Service]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cable Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China PA System Power Amplifier with Enough Rated Power]

Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dark Green Plastic PVC Film for Christmas Tree Leaves-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sud1000h Poly Welder Butt Fusion Machine]

Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers]

Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, Part I.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Treatment Anthracite.Heat Treated Welding Protection Glassfiber Cloth Ht800, 175-184[Reinforcement Net Non Woven Polyester Laid Scrim]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Activated Carbon Strong Effect Peeling off Face Mask Mud]

Whitaker,J.R.(1963),Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. Analytical Chemistry35(12), 1950-1953[Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nutrition Enhancer Environmental Nutritional Yeast PowderAuthor for correspondence