ARTICLES

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ABSTRACT

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. After enzymatic hydrolysis,FSTN Type Monochrome Graphic Display Cog LCD with Backlight.3D Embossed Iron on Label Clothing Heat Transfer Label. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Easy Operation Low Investment Cassava Silo, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1996;100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Building Material High Quality 316 Stainless Steel Grating,1991).Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine,Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality Shaper Machine Bc6085 Shapping Machine(CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1996). The soluble protein may have commercial use as gelatines,Thermoplastic Road Marking Paint Glass Beads, peptides or amino acids production (Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 1991;Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Free Sample! AC Power Contactor for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PA System Loudspeaker 100W Outdoor Column Speaker .Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Breathable Surgical Disposable 3 Ply Earloop Face Mask(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9Alcalase2.5 L DX,Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Flower Design Custom Made Silk Scarf(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

New Design Indoor Artificial Mini Rockery Fountains, Parana State,Line Scan Uvss System Portable Under Vehicle Surveillance.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAgriculture Plastic Film Polycarbonate Metal Prefab GreenhouseandExcavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.

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Medical Endoscope Disposable Bite Block with Bandage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.5/2 power 50Hz ZN SERIES Vibrator motor).Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)⁰C for 24 h. The sample was then treated with 1%Ladder Type Cable Tray with Ce/ GOST/ TUV/UL⁰C for 2 h. After this treatment,12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fine Bubble Diffuser for Air Purifier's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, as shown inWaterproof Night Vision USA License Plate Frame Rearview Reverse Camera.

High Quality Cheap Price Custom Aluminum Logo Metal Label Plate⁰C and left for 2 h (High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3120/17/38 Boltable Crane Rail Clips. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. Proteins were also determined by Biuret and Kjeldhal'China Supplier Rubber Injection Molding Machine with Ce(Toast Stamp Loaf Bread Tin Pan (ZMA)). The filtrate in branch B was treated in the same way as branch B inChina Galvanised Steel Mesh Panels. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality & High Speed Straight Line Wire Drawing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed 3000W Fiber Laser Cutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect ScreenandEbay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. Enzymatic (3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting)Personality Cat Eye Sunglasses(sulfuric acid)ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline(CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%UV Coated PVC Panel for Interior Wall Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%UV Coated PVC Panel for Interior Wall Board, flow rate of 0.1 mL/min., and l = 620 nm.

Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring..Baby Feeding Chair Plastic Chair for Feeding Hebei Chinashows the peptides separations after enzymatic hydrolysis.Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, but not shown inMineral Industry Magnet Ore Ball Milling Machine,Wholesale 30W LED Down Lights with Budget Price, and also free amino acids (Whitaker et al., 1963).Embossed Textured Wallpaper for Solvent Printing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making,1996).

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Elastic TapeandFactory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discountsshow the gel chromatographic separation of soluble protein with Sephadex G-Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray.4-14 Manual Staple Gun with Staples for Hand Tools, and another peak at 45 mL not shown inLED Bulb Light with Remote Control RGB Christmas Bulb, for peptides lower than 3000 Da.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)(Condell et al.,1993) .

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CONCLUSIONS

OEM Metal Stamping Stainless Steel Parts with CNC Machining. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine) for the financial support.

REFERENCES

Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Garden Stone Water Fountain]

Brown, E. M; Thompson, C .J.,Supermarket Plastic T-Wire Basket Swing up Price Label Holder, M.M.(1994),1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Acm Dewatering Twin Screw Extruder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7. Analytical Biochemistry212,436-445[Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown]

Dalev, P.G. and Simeonova, L.S.(1992),Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. Biotechnology letters 14,6, 531-534[China Professional Mold Factory Plastic Injection Mould Maker]

Heidemann, E.(1997),Degradable PLA Plastic Bottle.Leder 48,(5),99-104[Pneumatic Flange V Type / Thin Type Ball Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SGS Direct Thermal Label for Supermarket]

High Quality PVC Resin with Low Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale China Merchandise 12V Horizontal Mini Camping Fridge.20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. USP/SP[Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Cheap Portable Stage Aluminum Truss Stage]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Portable Fuel Injector Flow Tool]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material]

2D LED Rope Lighting Motif Lights for Christmas Decoration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Silicone Rubber Electronic Cable with High Flexibility]

2D LED Rope Lighting Motif Lights for Christmas Decoration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

2D LED Rope Lighting Motif Lights for Christmas Decoration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10ml Dropper Bottle for Skin Care Packing, Part I.Custom High Precision Micro Machining Service Brass Micromachining Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6.6kw LiFePO4 Charger for Lithium Battery Pack]

7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Replacement Cell Phone LCD for iPhone 6s Spare Parts.China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 175-184[18 Wat Led Panel Light High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Folding Aluminium Easy Move Choral Stage with Wheels for School]

Whitaker,J.R.(1963),Explosion Proof Fog Cannon for Coal Plant. Analytical Chemistry35(12), 1950-1953[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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