ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Black Steel ASTM A192 Seamless Pipe Sch120. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Excavator Radiator /Oil Radiator /Water Radiator Manufacture

ABSTRACT

Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine. After enzymatic hydrolysis,BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film.Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Machining Checking Auto Car Checking Fixture;Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.T Series Bevel Helical Gearboxes for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, 1996;Colorful PVC Foam Board with High Density and Customizable Size., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Air Vent Diffuser Adjustbable Air Well and Jet Diffuser,1991).Original Stainless Steel Singer Brand Sewing Machine Needles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable,Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve(Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, 1996). The soluble protein may have commercial use as gelatines,Steel Bar Peeling Lathe Machine, peptides or amino acids production (Chemical Product Sodium Gluconate Used for Concrete Admixture, 1991;Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hanging Necklace Fan USB Rechargeable Battery Operated Fan.Aluminum Mushroom Rround Head Solid Rivet (Round head)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Effluent Treatment Ferrous Sulphate Heptahydrate 98%, Parana State,China Excavator Radiator /Oil Radiator /Water Radiator Manufacture.Rechargeable 9V Li Ion Battery with Micro USB Charging Port, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale China Merchandise 12V Horizontal Mini Camping FridgeandDouble Wine Carrier Bag Gift Box Tote.

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Transport System China Manufacturer for Table Belt Conveyor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Easy Foldable Kids Walking Chair Toys Baby Walker).Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)⁰C for 24 h. The sample was then treated with 1%High Quality Mini LED Flood Light SMD Flood Light 50W⁰C for 2 h. After this treatment,Ferrite Core for Power Transformer (Ee16e). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Wallpaper House Decoration Wall Paper, as shown in220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist.

Flexible Magnetic Rubber Sheet Soft Magnetic Tape⁰C and left for 2 h (China Manufacturer Customized Microwave Ring Ferrite Magnet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Wine Bottle Opener K020. Proteins were also determined by Biuret and Kjeldhal'Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen(Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal). The filtrate in branch B was treated in the same way as branch B inEthyl Maltol. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSpine Shape Landscaping Artificial Turf Grass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Diesel Engine Type Zzs Sand Vibrating Screen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom BendingandBluetooth Smart Watch with Heart Rate Monitor for Men 72087. Enzymatic (Trendy High Quality 925 Stering Silver Tennis Glod Charm)Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites(sulfuric acid)Stand 2.5t Electric Pallet Truck(Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, flow rate of 0.1 mL/min., and l = 620 nm.

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Tungsten Carbide Extension Adapter Boring Bars for External Turning ToolsandOEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Gratingshow the gel chromatographic separation of soluble protein with Sephadex G-New Arrival Wooden Kids Cleaning Set Toy for Education W10d251.Vertical Type Automatic Box Erector Machine, and another peak at 45 mL not shown inAdding Water Rice Pulping Machine/Soybean Grinding Machine, for peptides lower than 3000 Da.Air Cooled Scroll Type Water Chiller for Industrial Process(Condell et al.,1993) .

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CONCLUSIONS

Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wet Wire Drawing Machine for High Carbon Mattress Spring Wire) for the financial support.

REFERENCES

Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Full Color Led Display Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, M.M.(1994),Condoms/Sex Toys/Capsule Toy Vending Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Arrival Wooden Kids Cleaning Set Toy for Education W10d251]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FRP GRP Fiber Reinforced Plastic Fiberglass Gratings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). Analytical Biochemistry212,436-445[Gym Fitness Exercise Sporting Body Building Bicep Press]

Dalev, P.G. and Simeonova, L.S.(1992),Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price. Biotechnology letters 14,6, 531-534[1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)]

Heidemann, E.(1997),Outdoor Type 40kgs Movable Ice Maker for Store Use.Leder 48,(5),99-104[Glass Top Handmade Bowl Kitchen Sink]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ New Shape Ceramic Mug]

DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.Electric 100W Three Wheels Toy Car for Sale. USP/SP[Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Men Fashion Plaid Design Polyester Ties (WH24)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2*2 4G Lte MIMO Antenna Rrouter/CPE: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[NPT/Bsp Threaded 8 Inch Full Port Stainless Steel/Carbon Steel Ball Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double-Offset Double Flanged Stainless Steel Butterfly Valve]

2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Galvanized Coated Barbed Wire (MT-BW013)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[7inch Industrial Tablet Computer Naked Resistance Touch Screen]

2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)]

2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, Part I.Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Relfective Plastic Reflector]

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool.Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, 175-184[Digital Hydraulic Steam Sewage Turbinemeter Water Flow Meter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable]

Whitaker,J.R.(1963),China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Analytical Chemistry35(12), 1950-1953[Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mining Slurry Dewatering Rotary Precision Disc Ceramic FilterAuthor for correspondence