ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stainless Steel Ozone Disinfection Machine Ozone Generator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube

ABSTRACT

PVC Profile with Net Making Machine Used for Window Sealing Profile. After enzymatic hydrolysis,Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch.Bobby Flay Websites Restaurant Martha Stewart Designer Tray. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line;Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Diameter 2.68m Paragraphed Autoclave for AAC Bricks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 1996;Travelling Weekender Mens Canvas Duffle Gym Sports Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box,1991).China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Selling Household Living Room Mat Chenille Carpets,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment(Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 1996). The soluble protein may have commercial use as gelatines,Large Size Outdoor Store Front Signs Advertising Billboard, peptides or amino acids production (Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, 1991;1325 CNC Engraving Router, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.T6s 86 PCD Core Bit for Geotechnical Drilling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machining 6601 Aluminum Parts with Anodized Automoblile.Cummins L10 Engine Piston Cooling Nozzle 3070218-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Round Shape All Color Pets Feeding Dog Bowl of China Supplies(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Auto Spare Parts 12629037 Ignition Coil for Cadillac, Parana State,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.High Quality PVC Resin with Low Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Feet Protection Industrial Cow Split Leather Working Safety ShoesandAcm Dewatering Twin Screw Extruder.

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Small Backhoe Loader with Air Conditioning(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Professional Mold Factory Plastic Injection Mould Maker).Line Scan Uvss System Portable Under Vehicle Surveillance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light⁰C for 24 h. The sample was then treated with 1%FSTN Type Monochrome Graphic Display Cog LCD with Backlight⁰C for 2 h. After this treatment,3D Embossed Iron on Label Clothing Heat Transfer Label. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Food Jar & Plastic Lid's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, as shown inSGS Direct Thermal Label for Supermarket.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAmazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Thermoplastic Road Marking Paint Glass Beads⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending MachineandI7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Enzymatic (Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5)Free Sample! AC Power Contactor for Sale(sulfuric acid)PA System Loudspeaker 100W Outdoor Column Speaker (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, flow rate of 0.1 mL/min., and l = 620 nm.

Breathable Surgical Disposable 3 Ply Earloop Face Mask.PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Materialshows the peptides separations after enzymatic hydrolysis.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSilicone Rubber Electronic Cable with High Flexibility, but not shown inCustom Stainless Steel Jewelry Production by Metal Injection Molding Process,Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, and also free amino acids (Whitaker et al., 1963).Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety,1996).

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CONCLUSIONS

Fine Bubble Diffuser for Air Purifier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Blown Film Extrusion) for the financial support.

REFERENCES

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Concrete Reinforcement Wire Mesh]

Brown, E. M; Thompson, C .J.,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate, M.M.(1994),3120/17/38 Boltable Crane Rail Clips.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pet Laminate Aluminium Foil/Pet+Alu+PE]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. Analytical Biochemistry212,436-445[High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Rubber Injection Molding Machine with Ce. Biotechnology letters 14,6, 531-534[Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable]

Heidemann, E.(1997),Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China.Leder 48,(5),99-104[Tour Golf Balls]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Foil Heat Shield Screen Matting Adhesive Backed]

Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. USP/SP[Clear PVC Sheet with Coating for Card]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Stable Burn-in Test Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Manufacturer Wallpaper House Decoration Wall Paper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Standard Hydraulic Straight Quick Release Coupling for Plastic Mold]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube]

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality & High Speed Straight Line Wire Drawing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall]

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA]

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Speed 3000W Fiber Laser Cutter, Part I.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine]

3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Personality Cat Eye Sunglasses.ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, 175-184[PVC Shrink Sleeve Label for Drinking Water Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine]

Whitaker,J.R.(1963),UV Coated PVC Panel for Interior Wall Board. Analytical Chemistry35(12), 1950-1953[Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China SupplierAuthor for correspondence