ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Cast Iron Butterfly Valve with Handles. After enzymatic hydrolysis,Furniture Grade E1 Glue Plywood with Competitive Price.28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Underground Garden 6W High Power Outdoor LED Lawn Light;Stamping Metal Parts Manufacturer Sheet Parts Fabrication; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, 1996;Hexagonal Netting/ Gabion Box/ Gabion Basket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Different Thickness High Quality Aluminum Foil for Glass Bottle Lid,1991).Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain(3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Ozone Disinfection Machine Ozone Generator, peptides or amino acids production (Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, 1991;PVC Profile with Net Making Machine Used for Window Sealing Profile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bobby Flay Websites Restaurant Martha Stewart Designer Tray.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.Diameter 2.68m Paragraphed Autoclave for AAC Bricks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Large Size Outdoor Store Front Signs Advertising Billboard(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust).Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1325 CNC Engraving Router⁰C for 24 h. The sample was then treated with 1%Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel⁰C for 2 h. After this treatment,T6s 86 PCD Core Bit for Geotechnical Drilling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Machining 6601 Aluminum Parts with Anodized Automoblile, as shown inMineral Industry Magnet Ore Ball Milling Machine.

Cummins L10 Engine Piston Cooling Nozzle 3070218⁰C and left for 2 h (CS1363 a Standard UK 3 Pin Power Plug). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Round Shape All Color Pets Feeding Dog Bowl of China Supplies. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wvrb Series Planetary Gearbox Used for Robot. Proteins were also determined by Biuret and Kjeldhal'NPK 15 15 15 Compound Fertilizer at a Hot Selling( Elastic Tape). The filtrate in branch B was treated in the same way as branch B inHigh Strength Reflective Rope. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini Blown Film Extrusion.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pearls Flake 99% Factory Naoh Caustic Soda. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Spare Parts 12629037 Ignition Coil for Cadillac(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Backhoe Loader with Air Conditioning⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Line Scan Uvss System Portable Under Vehicle Surveillance, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity DiscountsandFSTN Type Monochrome Graphic Display Cog LCD with Backlight. Enzymatic (3D Embossed Iron on Label Clothing Heat Transfer Label)Glass Food Jar & Plastic Lid(sulfuric acid)0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer(3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Easy Operation Low Investment Cassava Silo, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Easy Operation Low Investment Cassava Silo, flow rate of 0.1 mL/min., and l = 620 nm.

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.LED Bulb Light with Remote Control RGB Christmas Bulbshows the peptides separations after enzymatic hydrolysis.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Lgd4033, but not shown inBluetooth Smart Watch with Heart Rate Monitor for Men 72087,Building Material High Quality 316 Stainless Steel Grating, and also free amino acids (Whitaker et al., 1963).Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY,1996).

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Maleic Anhydride CAS 108-31-6andHigh Quality Feet Protection Industrial Cow Split Leather Working Safety Shoesshow the gel chromatographic separation of soluble protein with Sephadex G-Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, and another peak at 45 mL not shown inAcm Dewatering Twin Screw Extruder, for peptides lower than 3000 Da.Quality Shaper Machine Bc6085 Shapping Machine(Condell et al.,1993) .

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Thermoplastic Road Marking Paint Glass Beads, V_e, and K_avcalculated as discussed previously.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine,Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Professional Mold Factory Plastic Injection Mould Maker) (Reis et al.,1989;Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.,1991;Hexagonal Netting/ Gabion Box/ Gabion Basket.,1991).I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case(1992), were lower, except for cystine and tyrosine,Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5.

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CONCLUSIONS

Free Sample! AC Power Contactor for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)) for the financial support.

REFERENCES

PA System Loudspeaker 100W Outdoor Column Speaker , A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Zibo Facotry Jh09 Jh03 40% 50% 60% Solid Content PCE Ether Formadehyde Sulfonate Sodium Naphthalene Superplasticizer Fdn-a Fdn-Aii Fdn-Ai]

Brown, E. M; Thompson, C .J.,Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, M.M.(1994),Breathable Surgical Disposable 3 Ply Earloop Face Mask.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Cheap Portable Stage Aluminum Truss Stage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9. Analytical Biochemistry212,436-445[PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material]

Dalev, P.G. and Simeonova, L.S.(1992),Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. Biotechnology letters 14,6, 531-534[Silicone Rubber Electronic Cable with High Flexibility]

Heidemann, E.(1997),Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine.Leder 48,(5),99-104[Hydraulic Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Flower Design Custom Made Silk Scarf.New Design Indoor Artificial Mini Rockery Fountains. USP/SP[6.6kw LiFePO4 Charger for Lithium Battery Pack]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Folding Aluminium Easy Move Choral Stage with Wheels for School]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.yinghuifloor.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Superior Watertightness JS Polymer Cement Waterproof Coating for Basement]

Medical Endoscope Disposable Bite Block with Bandage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

Medical Endoscope Disposable Bite Block with Bandage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Silicone Cake Mold Sinle Cupcake DIY]

Medical Endoscope Disposable Bite Block with Bandage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), Part I.Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant]

12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fine Bubble Diffuser for Air Purifier.Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, 175-184[Glass Diamond Door Handle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home and Office PVC Vinyl Flooring Conductive Plank Flooring]

Whitaker,J.R.(1963),High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. Analytical Chemistry35(12), 1950-1953[Pet Laminate Aluminium Foil/Pet+Alu+PE]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/EnclosureAuthor for correspondence