ARTICLES

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ABSTRACT

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. After enzymatic hydrolysis,Degradable PLA Plastic Bottle.Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House;No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2D LED Rope Lighting Motif Lights for Christmas Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, 1996;10ml Dropper Bottle for Skin Care Packing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom High Precision Micro Machining Service Brass Micromachining Parts,1991).7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Replacement Cell Phone LCD for iPhone 6s Spare Parts,China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Explosion Proof Fog Cannon for Coal Plant(3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, 1996). The soluble protein may have commercial use as gelatines,Silicone Large Non-Stick BPA Free Baking Mat Sheet, peptides or amino acids production (19mic Black Cast PE Stretch Film for Pallet Wrapping, 1991;Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Design Plush Panda Animal Slippers Comfort Foam Support by., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best 6-Axis Electric Screwdriver Machine Robot.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence.FTTH Embedded Type Sc Fiber Optic Fast Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multi-Function Small Tree Digging Shovel/Tree Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PS 200 Upper Discharge Centrifuge, Parana State,Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)and Black Epoxy.

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Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Produce N2o for Medical Gas Equipments).Electrosurgical Generator High Frequency Electricity Kinfe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Viton O Ring Kit with Excellent with Oil Resistance⁰C for 24 h. The sample was then treated with 1%One Step HBV 5 in 1 Rapid Test⁰C for 2 h. After this treatment,Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Summer Fresh Landscaping Turf Lawn's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pipe Sheet Laser Cutter with Tube Cutting, as shown inBobcat Skid Steer Loader Bucket Teeth 6737325lp.

Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan⁰C and left for 2 h (2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Supply Christmas Lace Gold Silver Offray Silk Ribbon. Proteins were also determined by Biuret and Kjeldhal'Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots(Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts). The filtrate in branch B was treated in the same way as branch B inTyco 3 Pin Waterproof Electrical Connector Cable Wiring Harness 282087 Wire Cable Harness. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Toast Stamp Loaf Bread Tin Pan (ZMA).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Baking Soda 99.8% Min Sodium Bicarbonate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine.Children Bear English Story Book Printingshows the peptides separations after enzymatic hydrolysis.Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in KTV, but not shown inCustom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet,Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950, and also free amino acids (Whitaker et al., 1963).Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls,1996).

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CONCLUSIONS

Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile) for the financial support.

REFERENCES

2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Square Pipe Making Machine]

Brown, E. M; Thompson, C .J.,Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant, M.M.(1994),150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wall Washer Light Building Decoration Landscape LED IP65 Linear Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[marble Makeup Brush Set for Travel in 20PCS]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Design and Nice Price All in One Solar Street Light. Analytical Biochemistry212,436-445[Round Casting Iron Trench Cover Manhole Cover Frame]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Large Ss Stamping/ Bending Sheet Metal Parts. Biotechnology letters 14,6, 531-534[iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[White Phytosterol 90%]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Design Indoor Artificial Mini Rockery Fountains]

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Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine]

Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle, Part I.Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

Whitaker,J.R.(1963),Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant. Analytical Chemistry35(12), 1950-1953[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wiskind Hot-Rolled Low Cost Commercial Steel Structure GreenhouseAuthor for correspondence