ARTICLES

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ABSTRACT

Railway Freight Wagon Spare Parts Casting and Forging for Bogie. After enzymatic hydrolysis,Rainsky Ceiling Mounted Stainless Steel Shower Heads.Custom Sizes Acrylic Shoes Storage Display Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Casting Cover Aluminum Die Casting Products;Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, 1996;Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut,1991).0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine,304 316 Stainless Steel Grooved Cross for Water,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole(SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, 1996). The soluble protein may have commercial use as gelatines,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, peptides or amino acids production (20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, 1991;Hot Sale Laser Projector RGB Laser Light for Stage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Acm Dewatering Twin Screw Extruder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).Tumble Belt Type Shot Blasting Machine for Iron Castings Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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DIN127 Stainless Steel Spring Lock Washer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Factory Queen Mattress with Visco Memory Foam and Latex).29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Condition Hot Sale Automatic Animal Feed Cooler Machine⁰C for 24 h. The sample was then treated with 1%Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System⁰C for 2 h. After this treatment,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Garlic Red Mesh Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, as shown in9~12 Inch Mechanical Backing Plate.

Ground Finish Molybdenum Tzm Rod⁰C and left for 2 h (Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Transparent/Clear BOPP Jumbo Roll. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. Proteins were also determined by Biuret and Kjeldhal'Chenille Microfiber Mop Head(7PCS RGBW LED Plastic PAR Light Wall Washer). The filtrate in branch B was treated in the same way as branch B inwww.yasentag.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Waterproof Pizza Sign Large Advertising Signboard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVC Shrink Sleeve Label for Drinking Water Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Customized Stainless Steel Parts by Deep Drawing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chicken Layer Cage Poultry Cage Chicken CagesandChildren Bear English Story Book Printing. Enzymatic (Flexible Board FPC PCB Camera Module Rigid Flex PCB)High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(sulfuric acid)Cherry Wooden Grain PVC Film in Furniture(SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV.Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thicknessshows the peptides separations after enzymatic hydrolysis.Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inASA-UPVC Anti-Corrosive Composite Roof Tile, but not shown inSleeved Wire Harness for Industry Equipment,Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, and also free amino acids (Whitaker et al., 1963).Linen Quiltcover Set with Quilting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment,1996).

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CONCLUSIONS

High-Quality Pre-Seasoned Cast Iron Wok. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa) for the financial support.

REFERENCES

2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pp Super Sack Recycled]

Brown, E. M; Thompson, C .J.,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, M.M.(1994),Marine High Visibility Solas Reflective Tape.The Journal of the American Leather Chemists Association (JALCA)89(7)[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Frame Hydraulic Press Machine for Bending Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. Analytical Biochemistry212,436-445[Familu Use Mini Small Pig Feed Pellet Mill]

Dalev, P.G. and Simeonova, L.S.(1992),Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). Biotechnology letters 14,6, 531-534[Cherry Wooden Grain PVC Film in Furniture]

Heidemann, E.(1997),Top Quality Waterproof IP66 Aluminum Die Cast Junction Box.Leder 48,(5),99-104[Decoration (HS-Y72)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Kindergarten Playground Preschool Kids Plastic Slide and Swing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Magic Tape Hook and Loop Fasteners.Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. USP/SP[Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Air Vent Diffuser Adjustbable Air Well and Jet Diffuser]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Snow Ball]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disk Filter System with Automatic Backwash Valves]

25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Starch Doy Bag Packaging Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Type 40kgs Movable Ice Maker for Store Use]

25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve]

25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), Part I.Fashion Soft PVC Rubber Keychains for Promation Gift. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cantilever Oxygen Plasma Cutter 80]

Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, 175-184[Purple Candle Container Glass Jar with Wooden Lid Colored Jars]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Transparent/Clear BOPP Jumbo Roll]

Whitaker,J.R.(1963),Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. Analytical Chemistry35(12), 1950-1953[81144m Thrust Roller Bearing Used for Vertical Machine Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatically Rotate The Makeup Brush with 360 Degrees and Multiple GearsAuthor for correspondence