ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Boned Polyester Woven Faux Suede Sofa Fabric. After enzymatic hydrolysis,Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making.20 Bottles Electric Wine Display Cooler. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High-Efficient Htk Walnut Shell Filter for Water Purification;Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Popular European Style White Marble Coffee Tables for Dinnig Room, 1996;High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System,1991).Kwikstage Scaffolding System-Diagonal Brace. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,360 Rotating Automatic Touchless Car Wash Equipment with Drying System,48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox(Popular European Style White Marble Coffee Tables for Dinnig Room, 1996). The soluble protein may have commercial use as gelatines,IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, peptides or amino acids production (Sell Rubber / EVA / PE/PVC Foam Board, 1991;Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Brake Disc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Popular European Style White Marble Coffee Tables for Dinnig Room, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote.Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Screen Display Wireless Charging 10000mAh Power Bank(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning, Parana State,Parts for Crane Outrigger Hydraulic Oil Cylinder.2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Popular European Style White Marble Coffee Tables for Dinnig Room, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)andPaper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.

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Alu 2319 Slimline 12mm Wide Recessed Profile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Discussion and Interpretation System).High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Size Christmas Gift Personalized Watch Boxes for Men⁰C for 24 h. The sample was then treated with 1%12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope⁰C for 2 h. After this treatment,8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115, as shown inUV Laser Marking Machine (with Air Cooling System).

China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)⁰C and left for 2 h (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Cross Recesed Flat Head Self Tapping Screw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Our Perforated Aluminum & Solid Panel for New Office Wall Cladding. Proteins were also determined by Biuret and Kjeldhal'2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone(Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace). The filtrate in branch B was treated in the same way as branch B inZinc door handle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Kids Trucker Hat, Kids Mesh Cap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wd190V Handheld Full Body Scanner Hand Held Metal Detector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Hydraulic Orbital Valve Power Steering(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/AISI 410 Stainless Steel Galvanized Wire for Making Scourer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CS1363 a Standard UK 3 Pin Power Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

100% Polyester Gabardine Shirting Fabric with Transfer PrintingTreeandFood Chopper. Enzymatic (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile)Wholesale Prices of 20W 40W 60W Solar Street Lights(sulfuric acid)ISO 3821 3/16′′ Twin Line Welding Hose for USA Market(Popular European Style White Marble Coffee Tables for Dinnig Room, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, flow rate of 0.1 mL/min., and l = 620 nm.

Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond.Copper Laminated Steel Strip T2/C11000shows the peptides separations after enzymatic hydrolysis.Factory Stock Spring Steel U Type Clip Nut(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Rubber Wrist Bands, but not shown inOutoodr Svgc Composite Compensation with Capacitors for Reactive Power,ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, and also free amino acids (Whitaker et al., 1963).Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Popular European Style White Marble Coffee Tables for Dinnig Room,1996).

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CONCLUSIONS

Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom LCD Screen Video Box for Package) for the financial support.

REFERENCES

Sanjing PVC Lining Layflat Fire Hose, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kettle Without Tank]

Brown, E. M; Thompson, C .J.,Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, M.M.(1994),China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Small Backhoe Loader with Air Conditioning]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic CNC Glass Cutting Machine Glass Cutting Table. Analytical Biochemistry212,436-445[4 Drawer Steel Office Vertical Filing Cabinet]

Dalev, P.G. and Simeonova, L.S.(1992),Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory. Biotechnology letters 14,6, 531-534[Insecticidal Lamp with Solar Energy Frequent Vibration]

Heidemann, E.(1997),Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken.Leder 48,(5),99-104[Polyglycolica Acid Absorbable Suture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml]

4 Drawer Steel Office Vertical Filing Cabinet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.Pet PP Rope and Broom Wire Drawing Machine. USP/SP[Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sequins Butterfly Handmade Beads Embroidery Patch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Burnout Tank Top Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[50mm Magnetic River Water Sewage Flow Meter for Acid]

PTFE Seat Wafer Butterfly Valve Pn16, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)]

PTFE Seat Wafer Butterfly Valve Pn16,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen]

PTFE Seat Wafer Butterfly Valve Pn16, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, Part I.Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House]

Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt.Plain Makeup Bag Canvas Cosmetic Bag, 175-184[Multi Sapphire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Family Party Form Camping Kitchen]

Whitaker,J.R.(1963),14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. Analytical Chemistry35(12), 1950-1953[Prefab Expandable Luxury Living Container House Prefabricated House]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link FenceAuthor for correspondence