ARTICLES

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ABSTRACT

Children Bear English Story Book Printing. After enzymatic hydrolysis,Flexible Board FPC PCB Camera Module Rigid Flex PCB.High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), 1996;Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Linen Quiltcover Set with Quilting,1991).2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace,Teerte Square Flat Head Self Tapping Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard(Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), 1996). The soluble protein may have commercial use as gelatines,Bto 22 Electric Galvanized Razor Concertina Wire for Military, peptides or amino acids production (Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, 1991;Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Military Large Event Marquee Tent Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-Quality Pre-Seasoned Cast Iron Wok.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.Marine High Visibility Solas Reflective Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:24L Thermoelectric Cooler and Warmer Car Mini Car Fridge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Starch Doy Bag Packaging Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Betaine Anhydrous 98% Food Grade Good Quality Competitive Price).High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Soft PVC Rubber Keychains for Promation Gift⁰C for 24 h. The sample was then treated with 1%Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper⁰C for 2 h. After this treatment,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, as shown inOutdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree.

Factory Supply Cheap Custom Bleached White Cotton Gloves⁰C and left for 2 h (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,304 &314 Stainless Steel Seamless Bending Tube. Proteins were also determined by Biuret and Kjeldhal'Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark(Explosion Proof Fog Cannon for Coal Plant). The filtrate in branch B was treated in the same way as branch B inwww.anebon.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Grey Full-Zip Cotton Mens Hoodies.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av7inch Industrial Tablet Computer Naked Resistance Touch Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement RingandIndustrial Grade General Purpose Titanium Dioxide Rutile/Anatase. Enzymatic (Fcy30 Transport Mini Tipping Dumper)Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park(sulfuric acid)Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)(Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Premium Grade Rubber Cloth Duct Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Premium Grade Rubber Cloth Duct Tape, flow rate of 0.1 mL/min., and l = 620 nm.

Sud1000h Poly Welder Butt Fusion Machine.Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Beltshows the peptides separations after enzymatic hydrolysis.Customized Anti-Rust Torsion Spring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSodium Gluconate 98% Min Industry Grade for Chemical Admixture, but not shown inAlumina Ball with High Quality,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, and also free amino acids (Whitaker et al., 1963).New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44),1996).

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Multicore Underground Aluminum Conductor Steel Tape Armored Power CableandBest Polishing Pads Concrete Grinding Discs Diamond Sanding Discsshow the gel chromatographic separation of soluble protein with Sephadex G-Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, and another peak at 45 mL not shown in330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing, for peptides lower than 3000 Da.PP Woven Bag, Rice, Beans, Plastic Bag(Condell et al.,1993) .

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China Professional Mold Factory Plastic Injection Mould Maker, V_e, and K_avcalculated as discussed previously.Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Medical Packing Bags) (Reis et al.,1989;Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.,1991;Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.,1991).Industrial Carbon Steel Wire Hose Hydraulic Pipe(1992), were lower, except for cystine and tyrosine,24k Gold Whitening Anti-Spot Mask.

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CONCLUSIONS

Clay Tiles Making Machine with Latest Technology. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Magic Tape Hook and Loop Fasteners) for the financial support.

REFERENCES

DIN Standard Gratings/Gavlanized Steel Grating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Water Paint Line for Wood Furniture Automatic Spray Painting Machine Automatic Painting Leveling Production Line Automatic Spraying UV Baking Line]

Brown, E. M; Thompson, C .J.,50mm Magnetic River Water Sewage Flow Meter for Acid, M.M.(1994),PE Biodegradable Sheet Product Line/Single Screw Extruder.The Journal of the American Leather Chemists Association (JALCA)89(7)[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Brake Disc]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-27L/Min Water Exchanger Heat Pump Mold Temperature Machine. Analytical Biochemistry212,436-445[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]

Dalev, P.G. and Simeonova, L.S.(1992),Felt Belt for Textile Compactor. Biotechnology letters 14,6, 531-534[High Quality Customized Aluminum Foil Shisha Sheet]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Mini LED Flood Light SMD Flood Light 50W]

Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Metal Soft Enamel Souvenir Metal Medal Sport Medallions.20L Water Tank Lid Mold 3D Printing Samples Available. USP/SP[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anti-High Pressure Insulated Pliers with PVC Dipped Handles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale High Quality Metal Car Mount Holder Phone Holder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.wanyingtools.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant]

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pre-Engineered Cage Broiler Poultry Farm Building]

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides]

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet Laminate Aluminium Foil/Pet+Alu+PE, Part I.6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Amazon Hot Selling Kids Playing Pop up Goal]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla]

Whitaker,J.R.(1963),Dark Green Plastic PVC Film for Christmas Tree Leaves. Analytical Chemistry35(12), 1950-1953[Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts DamperAuthor for correspondence