ARTICLES

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ABSTRACT

Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies. After enzymatic hydrolysis,Auto Spare Parts Ignition Coil Fp85-18-100c.vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Enameled Mini Cast Iron Casserole, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality Tomato Sauce Making Machine, 1996;Vitamin B1 HCl 98% Feed Grade., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2018 TPR Clean Teeth Training Dog Pet Chew Toy,1991).Good Quality Zinc Plated Slotted Hex Head Roofing Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine,5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer(Good Quality Tomato Sauce Making Machine, 1996). The soluble protein may have commercial use as gelatines,High Quality Orthopedic Fiberglass Splint, peptides or amino acids production (Amazon Hot Selling Kids Playing Pop up Goal, 1991;Small Frame Hydraulic Press Machine for Bending Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3.5-7.0g Ozone Generator Purification ---Commercial., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Baby Feeding Chair Plastic Chair.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reflective Hand Hold Thermoplastic Road Marking. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality Tomato Sauce Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade Stainless Steel Threated Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

JIS S Type Cast Iron Water Filter Dn25, Parana State,New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238.Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Quality Tomato Sauce Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHydraulic Orbital Valve Power SteeringandGyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.

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Factor Direct Adult Sex Toy Doll for Male Sex(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hanging Necklace Fan USB Rechargeable Battery Operated Fan).C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory⁰C for 24 h. The sample was then treated with 1%WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W⁰C for 2 h. After this treatment,Worm Gear Fluorine Lined Butterfly Valve Flanged Type. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Poultry House, Livestock, Poultry Farm, as shown inChicken Farming Materials Poultry Battery Cages Layer Battery Cage.

1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine⁰C and left for 2 h (OPP Printed Block Bottom Bags). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Black Slim PU Leather Passport Travel Wallet. Proteins were also determined by Biuret and Kjeldhal'ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe(Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle). The filtrate in branch B was treated in the same way as branch B inDurable Firm Poultry House Steel Structure Building for 10000 Chickens in Botswana. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1.3KG Fire breaker for kitchen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Korean Integrated CTO Activated Carbon Filter for Water Treatment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vacuum Squeeze Filter Press for Coal Flotation Tailings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modular Portable Shipping Container Lkit Building Home Villa House, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Double-Offset Double Flanged Stainless Steel Butterfly Valve.Hot Sale Electric LED light Aroma Diffusershows the peptides separations after enzymatic hydrolysis.Automotive Acrylic Wholesale Masking Tape Factory Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMilitary Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, but not shown inPolycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer,Home Decoration Candle Glass Holder for Table Decorative, and also free amino acids (Whitaker et al., 1963).Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality Tomato Sauce Making Machine,1996).

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CONCLUSIONS

Boned Polyester Woven Faux Suede Sofa Fabric. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Suction Function Handheld Cool and Heat Food Bowl for Baby) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,20 Bottles Electric Wine Display Cooler, M.M.(1994),High-Efficient Htk Walnut Shell Filter for Water Purification.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Performance Multifunctional Crawler Mini Skid Steer Loader]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576). Analytical Biochemistry212,436-445[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

Dalev, P.G. and Simeonova, L.S.(1992),Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). Biotechnology letters 14,6, 531-534[Top China Forklift Supplier & Manufacturer]

Heidemann, E.(1997),Popular European Style White Marble Coffee Tables for Dinnig Room.Leder 48,(5),99-104[Cat3 Voice Modular Jack (FINISHED)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box]

Ep/Nn/EPP Industrial Rubber Conveyor Belt, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket.FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. USP/SP[Hot Sale Laser Projector RGB Laser Light for Stage]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kwikstage Scaffolding System-Diagonal Brace: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Health Care Product Export To China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

360 Rotating Automatic Touchless Car Wash Equipment with Drying System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ID25mm Od86mm Standard Through Hole Slip Ring]

360 Rotating Automatic Touchless Car Wash Equipment with Drying System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

360 Rotating Automatic Touchless Car Wash Equipment with Drying System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, Part I.IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door]

Sell Rubber / EVA / PE/PVC Foam Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sodium Gluconate 98% Min Industry Grade for Chemical Admixture.Brake Disc, 175-184[Customized Logo Gym Room 4/6/8mm Colorful TPE Yoga Mat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

Whitaker,J.R.(1963),DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. Analytical Chemistry35(12), 1950-1953[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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