ARTICLES

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ABSTRACT

High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry. After enzymatic hydrolysis,6.6kw LiFePO4 Charger for Lithium Battery Pack.Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Stable Burn-in Test Equipment, 1996;Wholesale Custom 100% Cotton Boys T-Shirt with Print., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industry 0.002mm Accuracy Checking Fixture,1991).Promotional Customized PP Hand Fan, Plastic Folding Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting,Six Stations Pen Press Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine(Customized Stable Burn-in Test Equipment, 1996). The soluble protein may have commercial use as gelatines,Vertical Automatic Granule Bag Packing Machine, peptides or amino acids production (Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, 1991;160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Manufacturing Tht/ Ai Double Wave Soldering Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Stable Burn-in Test Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manual Sealing Machine for Instant Noodles Paper Soup Bowls.Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, Parana State,Rectangular Stainless Steel Pipe SS304.Customized Hot Forging Agricultural Spare Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized Stable Burn-in Test Equipment, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom High Precision Micro Machining Service Brass Micromachining PartsandCustom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close.

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16mm 12 Strand UHMWPE Synthetic Fiber Rope(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt).High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vertical Type Caseformer Machine WJ-LKX-15⁰C for 24 h. The sample was then treated with 1%Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine⁰C for 2 h. After this treatment,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)0.1mm Tungsten Carbide 2f Single Flute Micro End Mill's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Agriculture Large Span Commerical Steel Frame Greenhouse, as shown inPlastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.

Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat⁰C and left for 2 h (Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. Proteins were also determined by Biuret and Kjeldhal'High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding). The filtrate in branch B was treated in the same way as branch B inHolographic Foil Hot Stamping Foil Cold Lamination Foil. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese Jbcz Handy Operation Threading Machine and Coupler. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPromotional Reusable Foldable PP Non Woven Shopping Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price Printing Doy Pack Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Disposable Non-Woven Dry Gloves Wipes Making Machine.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factoryshows the peptides separations after enzymatic hydrolysis.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inProfessional Gym Equipment Hammer Strength PRO Style Adjustable Bench, but not shown inCustomized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip,Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, and also free amino acids (Whitaker et al., 1963).Bottom Pushing for Fire Extinguisher Hot Spinning Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Stable Burn-in Test Equipment,1996).

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CONCLUSIONS

Pond Self Priming Booster suction  Water Pump with Pressure Switch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Factory Queen Mattress with Visco Memory Foam and Latex) for the financial support.

REFERENCES

Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3d Puffy Sticker]

Brown, E. M; Thompson, C .J.,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, M.M.(1994),for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. Analytical Biochemistry212,436-445[I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud]

Dalev, P.G. and Simeonova, L.S.(1992),School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). Biotechnology letters 14,6, 531-534[450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting]

Heidemann, E.(1997),Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle.Leder 48,(5),99-104[Hdc-He-24 Waterproof Aluminium 24 Pins Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-125kHz RFID Hotel Key Card with T5577 Chip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.yuyancable.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)]

USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters]

USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Biodegradable and Recycled Paper Straws]

USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, Part I.Wholesale High Pressure 80L Nitrogen Gas Cylinder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Construction & Decoration ERW Square Steel Tube Manufacturer]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[M4X10 Round Head Core Pulling Aluminum Nail]

Whitaker,J.R.(1963),Phillip Countersunk Head Screw Hot DIP Galvanized. Analytical Chemistry35(12), 1950-1953[EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Metal Window ScreenAuthor for correspondence