ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Quality Casting Cover Aluminum Die Casting Products. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags

ABSTRACT

304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. After enzymatic hydrolysis,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut;0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (304 316 Stainless Steel Grooved Cross for Water, 1996;Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home,1991).20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Laser Projector RGB Laser Light for Stage,Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer(304 316 Stainless Steel Grooved Cross for Water, 1996). The soluble protein may have commercial use as gelatines,Acm Dewatering Twin Screw Extruder, peptides or amino acids production (Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), 1991;Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality Good Price China Plastic Injection Molding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pet Hair Remover Glove for Dog, Grooming Gloves Brush. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (304 316 Stainless Steel Grooved Cross for Water, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.Alumina Balls for Hydogen Peroxide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti-High Pressure Insulated Pliers with PVC Dipped Handles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Garlic Red Mesh Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads).Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ground Finish Molybdenum Tzm Rod⁰C for 24 h. The sample was then treated with 1%Transparent/Clear BOPP Jumbo Roll⁰C for 2 h. After this treatment,Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chenille Microfiber Mop Head's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Waterproof Pizza Sign Large Advertising Signboard, as shown inFlexible Board FPC PCB Camera Module Rigid Flex PCB.

PVC Shrink Sleeve Label for Drinking Water Bottle⁰C and left for 2 h (Baking Soda 99.8% Min Sodium Bicarbonate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Galvanized Square Wire Mesh, Metal Window Screen. Proteins were also determined by Biuret and Kjeldhal'Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory(High Quality PVC Resin with Low Price). The filtrate in branch B was treated in the same way as branch B inEnig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Customized Stainless Steel Parts by Deep Drawing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChildren Bear English Story Book Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cherry Wooden Grain PVC Film in Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High-Quality Pre-Seasoned Cast Iron Wok, V_e, and K_avcalculated as discussed previously.2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears) (Reis et al.,1989;Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).,1991;Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.,1991).Marine High Visibility Solas Reflective Tape(1992), were lower, except for cystine and tyrosine,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.

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CONCLUSIONS

Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom) for the financial support.

REFERENCES

Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[E0 Ecological Board]

Brown, E. M; Thompson, C .J.,Magic Tape Hook and Loop Fasteners, M.M.(1994),Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.The Journal of the American Leather Chemists Association (JALCA)89(7)[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Transport System China Manufacturer for Table Belt Conveyor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). Analytical Biochemistry212,436-445[Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover]

Dalev, P.G. and Simeonova, L.S.(1992),25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Biotechnology letters 14,6, 531-534[Water Treatment Anthracite]

Heidemann, E.(1997),Starch Doy Bag Packaging Machine.Leder 48,(5),99-104[Universal Power Window Switch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fcy30 Transport Mini Tipping Dumper]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[G-800/1080 Tissue Box Crease Window Film Sticking Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine]

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women]

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine]

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, Part I.Factory Supply Cheap Custom Bleached White Cotton Gloves. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20 Bottles Electric Wine Display Cooler]

2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),304 &314 Stainless Steel Seamless Bending Tube.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, 175-184[China Manufacturer Large Glass Greenhouse Panels for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Price Gi/PPGI Roofing Sheet in China]

Whitaker,J.R.(1963),Custom Grey Full-Zip Cotton Mens Hoodies. Analytical Chemistry35(12), 1950-1953[ Cheap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)Author for correspondence