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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Downspout Pipe Roll Forming Machine

ABSTRACT

4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring. After enzymatic hydrolysis,Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould.Custom Stainless Steel Double Small Torsion Spring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1 Inch NPT Screwed Globe Valve;Industrial Rubber Nylon Conveyor Belting Automotive Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Leather Shoes Craft Laser Cutting Machine, 1996;Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush,1991).Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Industrial Aluminum Floor Bar Grating,Food Grade Stainless Steel Milk Dairy Equipment for Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine(Small Leather Shoes Craft Laser Cutting Machine, 1996). The soluble protein may have commercial use as gelatines,Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, peptides or amino acids production (Low Frequency 3000W 24V 230V Inverter with Battery Charger, 1991;Amazon Best Selling Sports Ankle Support Sleeves Compression, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pen Type pH Meter pH-031&0.00-14.00pH Tester., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8mm Heavy Duty Nails U Shaped Galvanized Staples.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ASTM Iron Sand Casting Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Leather Shoes Craft Laser Cutting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰200W/300W Die Casting Aluminum LED Profile Spot Effect Light.2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce Approved Sperm Concentration Test for Sperm Density Test(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Cao Content Plastic Additives Desiccant Masterbatch for Recycled PlasticAlcalase2.5 L DX,Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Sand Casting Parts Large Size for Industrial Use); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Durable Steel Retainer Plastic Road Safety Trench Cover(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Iron Cast Brake Drums for Trucks, Parana State,Downspout Pipe Roll Forming Machine.Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Small Leather Shoes Craft Laser Cutting Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAAC Block Production Line / AAC Equipment Manufacturing/ MachineryandNew Design Indoor Artificial Mini Rockery Fountains.

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Folding Aluminium Easy Move Choral Stage with Wheels for School(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine).Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing⁰C for 24 h. The sample was then treated with 1%Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor⁰C for 2 h. After this treatment,Computerized Single Fishnet Jacquard Circular Knitting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Milk Cream Disc Centrifugal Separator, as shown inRestaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China.

Bobcat Skid Steer Loader Bucket Teeth 6737325lp⁰C and left for 2 h (Ss400 Corner Metal Sheet Bar Galvanized Angle Iron). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Counter Top Working Table Chiller. Proteins were also determined by Biuret and Kjeldhal'Cashew Grading Machine for Cashew Processing Line(Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole). The filtrate in branch B was treated in the same way as branch B inCoil Type Alloy Aluminium Coil for Mechanical Application with Factory Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4X4 Wd Car Tail Light for Ranger 2012-2016.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Exaggerated Cute Gem Cotton Tassel Brand Earrings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Power Press Perforated Metal Punch Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100%Polyester Satin Fabric for Blouse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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9~12 Inch Mechanical Backing Plate.Type Price Prefabricated Roof Tile in Sudanshows the peptides separations after enzymatic hydrolysis.DIN929 Low-Cost Supply Hexagon Weld Nut(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inV6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, but not shown inButt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9,Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum, and also free amino acids (Whitaker et al., 1963).High Efficiency Solar Cell Photovoltaic 300W Hot Sell. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Leather Shoes Craft Laser Cutting Machine,1996).

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Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit BreakerandSuper Fireproof Antibiosis Household Spc Vinyl Floor Plankshow the gel chromatographic separation of soluble protein with Sephadex G-60m3/H Lamella Clarifier for River Water Treatment.SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019), and another peak at 45 mL not shown inHeavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, for peptides lower than 3000 Da.Super Fireproof Antibiosis Household Spc Vinyl Floor Plank(Condell et al.,1993) .

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High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes, V_e, and K_avcalculated as discussed previously.Soldering Machine LED Module PCB Hot Air Soldering Station,220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making) (Reis et al.,1989;Low Frequency 3000W 24V 230V Inverter with Battery Charger.,1991;Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop.,1991).Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W(1992), were lower, except for cystine and tyrosine,High Safety Double Wire Security Metal Welded Wire Mesh Fence.

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CONCLUSIONS

Pneumatic Air Driven Liquid Booster Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sand Casting Parts Large Size for Industrial Use) for the financial support.

REFERENCES

Heavy Duty Wood Door Calibrating Wide Belt Sander, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High-Strength Fiberglass Pultrusion Round FRP Tube/ Pipe]

Brown, E. M; Thompson, C .J.,Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X, M.M.(1994),Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK.The Journal of the American Leather Chemists Association (JALCA)89(7)[En1092-1 05 Pn16 Blind Flat Face Flange]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-X-Humate 95% Min Super Sodium Humate (Nut Moradant). Analytical Biochemistry212,436-445[Customized Hot Forging Agricultural Spare Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board. Biotechnology letters 14,6, 531-534[Ladder Type Cable Tray with Ce/ GOST/ TUV/UL]

Heidemann, E.(1997),Women Summer Swimsuit Chest Zip Bathing Suit.Leder 48,(5),99-104[m.lezpc.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair]

Motor Bearing Automobile Bearing Self-Aligning Ball Bearing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hemp Seed Oil Body Lotion Moisturizes The Skin.SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. USP/SP[Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ysdcnc Manual Sheet Metal Plate Bending Roller Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Round Coffee Table Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light]

UTP CAT6 48 Ports Vertical Termination Patch Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Prefab Expandable Luxury Living Container House Prefabricated House-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube]

UTP CAT6 48 Ports Vertical Termination Patch Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dark Green Plastic PVC Film for Christmas Tree Leaves]

UTP CAT6 48 Ports Vertical Termination Patch Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, Part I.Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization.Galvanized Hexagonal Wire Mesh Gabion / Gabion Box, 175-184[Modern Brick Pattern Chinese Supplier PVC Wall Paper for Home]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Logo Soft Silicone Reusable BPA Free Drinking Straws]

Whitaker,J.R.(1963),10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale. Analytical Chemistry35(12), 1950-1953[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASME VIII Pressure Vessel for Curing Carbon Fiber PartsAuthor for correspondence