ARTICLES

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ABSTRACT

Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. After enzymatic hydrolysis,The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine.Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, 1996;New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale,1991).Taiding Custom Tank Sheet Metal Fabrication. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Airtight Candy Bin with Scoop,Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell(Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, 1996). The soluble protein may have commercial use as gelatines,3 Layer Round Tea Tin with Air Tight Lid, peptides or amino acids production (China PA System Power Amplifier with Enough Rated Power, 1991;Export Grade Citric Acid Monohydrate as Detergent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Familu Use Mini Small Pig Feed Pellet Mill.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer.Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hairdressing Equipment Takara Belmont Barber Chair High Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment, Parana State,Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor.Rectangular Box Facial Tissue Paper Fk-110, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theExcellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical MillsandHome Kitchen Water Purification Ozone Generator 1000mg.

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SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag).Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%AAC Block Production Line / AAC Equipment Manufacturing/ Machinery⁰C for 24 h. The sample was then treated with 1%CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine⁰C for 2 h. After this treatment,Q32 Series Tumble Belt/Crawler Shot Blasting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Bar Grating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Custom Self Adhesive Leather Patch for Jackets, as shown inThermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFrozen Soybean in Pod(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof EVA Rubber Roofing Material for Shingle Building⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Gray Cast Iron Sand Casting Products with Universal Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/U. S. Type Drop Forged Wire Rope Clip, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System, V_e, and K_avcalculated as discussed previously.Sexy Mesh Transparent Female Running Tank Top with Hood Custom,Color Cardstock Color Paper Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam) (Reis et al.,1989;China PA System Power Amplifier with Enough Rated Power.,1991;New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress.,1991).Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts(1992), were lower, except for cystine and tyrosine,Home and Office PVC Vinyl Flooring Conductive Plank Flooring.

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CONCLUSIONS

Precision Engineering Parts Bearing Support Unit for Ball Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Customized Aluminum Foil Shisha Sheet) for the financial support.

REFERENCES

LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Royal Euro Top Cotton Fabric Mattress for Household]

Brown, E. M; Thompson, C .J.,165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, M.M.(1994),Silicone Cake Mold Sinle Cupcake DIY.The Journal of the American Leather Chemists Association (JALCA)89(7)[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting. Analytical Biochemistry212,436-445[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

Dalev, P.G. and Simeonova, L.S.(1992),10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing. Biotechnology letters 14,6, 531-534[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

Heidemann, E.(1997),Cross Stripe Sleeveless Yoga Wear.Leder 48,(5),99-104[Seafood Drying Machine / Cucumber Drying Machine / Kelp Drying Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

Polyester / Polypropylene Woven Geotextile for Road Construction, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Construction & Decoration ERW Square Steel Tube Manufacturer.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. USP/SP[DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Black Steel ASTM A192 Seamless Pipe Sch120: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Interior Metal 3D Bee Garden Ornament Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Pouch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factor Direct Adult Sex Toy Doll for Male Sex]

China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UTP CAT6 48 Ports Vertical Termination Patch Panel]

China Excavator Radiator /Oil Radiator /Water Radiator Manufacture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminium Unitized Curtain Wall for Office Building]

China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, Part I.Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating]

CNC Machining Checking Auto Car Checking Fixture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle.T Series Bevel Helical Gearboxes for Sale, 175-184[Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Iron Sheet Metal CNC Press Brake]

Whitaker,J.R.(1963),Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest. Analytical Chemistry35(12), 1950-1953[Milk Cream Disc Centrifugal Separator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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