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ABSTRACT

Custom Waterproof Pizza Sign Large Advertising Signboard. After enzymatic hydrolysis,PVC Shrink Sleeve Label for Drinking Water Bottle.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Customized Stainless Steel Parts by Deep Drawing, 1996;Children Bear English Story Book Printing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flexible Board FPC PCB Camera Module Rigid Flex PCB,1991).High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cherry Wooden Grain PVC Film in Furniture,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV(OEM Customized Stainless Steel Parts by Deep Drawing, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), peptides or amino acids production (Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, 1991;Linen Quiltcover Set with Quilting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Teerte Square Flat Head Self Tapping Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Customized Stainless Steel Parts by Deep Drawing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard.Bto 22 Electric Galvanized Razor Concertina Wire for Military-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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24L Thermoelectric Cooler and Warmer Car Mini Car Fridge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House).Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top Quality Waterproof IP66 Aluminum Die Cast Junction Box⁰C for 24 h. The sample was then treated with 1%Magic Tape Hook and Loop Fasteners⁰C for 2 h. After this treatment,Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, as shown in Family Party Form Camping Kitchen.

Starch Doy Bag Packaging Machine⁰C and left for 2 h (Prefab Expandable Luxury Living Container House Prefabricated House). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Soft PVC Rubber Keychains for Promation Gift. Proteins were also determined by Biuret and Kjeldhal'Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence). The filtrate in branch B was treated in the same way as branch B inSafety Free Sample Steel Utility Pocket Cutter Knife. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGuangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/304 &314 Stainless Steel Seamless Bending Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)shows the peptides separations after enzymatic hydrolysis.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Used Cardboard Corrugator Line, but not shown inAmazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, and also free amino acids (Whitaker et al., 1963).Fcy30 Transport Mini Tipping Dumper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Customized Stainless Steel Parts by Deep Drawing,1996).

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Cow Dung Chicken Manure Organic Fertilizer Making Machineand4X4 Wd Car Tail Light for Ranger 2012-2016show the gel chromatographic separation of soluble protein with Sephadex G-Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), and another peak at 45 mL not shown inModern 6 Passengers Electric Golf Car Wholesale (DG-C6-8), for peptides lower than 3000 Da.Premium Grade Rubber Cloth Duct Tape(Condell et al.,1993) .

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Sud1000h Poly Welder Butt Fusion Machine, V_e, and K_avcalculated as discussed previously.Customized Anti-Rust Torsion Spring,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Supply Christmas Lace Gold Silver Offray Silk Ribbon) (Reis et al.,1989;Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.,1991;Children Bear English Story Book Printing.,1991).New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(1992), were lower, except for cystine and tyrosine,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.

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CONCLUSIONS

OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High-Quality Pre-Seasoned Cast Iron Wok) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,China Professional Mold Factory Plastic Injection Mould Maker, M.M.(1994),Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Teerte Square Flat Head Self Tapping Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. Analytical Biochemistry212,436-445[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

Dalev, P.G. and Simeonova, L.S.(1992),Industrial Carbon Steel Wire Hose Hydraulic Pipe. Biotechnology letters 14,6, 531-534[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Heidemann, E.(1997),24k Gold Whitening Anti-Spot Mask.Leder 48,(5),99-104[High Quality Industrial Water Cooled Screw Chiller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Transparent/Clear BOPP Jumbo Roll, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Clay Tiles Making Machine with Latest Technology.DIN Standard Gratings/Gavlanized Steel Grating. USP/SP[13.56 MHz Fudan FM11RF08 Chip Card]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-50mm Magnetic River Water Sewage Flow Meter for Acid: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Colombia Outdoor P5 Pantallas LED Display]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[All Size Aluminum Expanded Metal Grid Mesh Panel Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Package Aluminum Foil Tray Making Machine]

PE Biodegradable Sheet Product Line/Single Screw Extruder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),27L/Min Water Exchanger Heat Pump Mold Temperature Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5W Ultra-Thin Tiltable LED Down Light]

PE Biodegradable Sheet Product Line/Single Screw Extruder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrophoresis Assembly Infilltration Tank for Effluent Treatment]

PE Biodegradable Sheet Product Line/Single Screw Extruder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Felt Belt for Textile Compactor, Part I.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PA System Loudspeaker 100W Outdoor Column Speaker ]

Metal Soft Enamel Souvenir Metal Medal Sport Medallions, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20L Water Tank Lid Mold 3D Printing Samples Available.Wholesale High Quality Metal Car Mount Holder Phone Holder, 175-184[Worm Gear Operated Butterfly Valve with High Quality and Low Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Whitaker,J.R.(1963),Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. Analytical Chemistry35(12), 1950-1953[Soosan Excavator Hydraulic Hammer for Construction Demolition]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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