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ABSTRACT

Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes. After enzymatic hydrolysis,Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine;304 316 Stainless Steel Grooved Cross for Water; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1996;20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Laser Projector RGB Laser Light for Stage,1991).Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer,Acm Dewatering Twin Screw Extruder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)(Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1996). The soluble protein may have commercial use as gelatines,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, peptides or amino acids production (Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, 1991;Good Quality Good Price China Plastic Injection Molding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pet Hair Remover Glove for Dog, Grooming Gloves Brush., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Alumina Balls for Hydogen Peroxide. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Anti-High Pressure Insulated Pliers with PVC Dipped Handles.Hot Selling Electronical Blue Pet Plastic Release Film Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DIN127 Stainless Steel Spring Lock Washer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Garlic Red Mesh Bag, Parana State,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBlack Steel ASTM A192 Seamless Pipe Sch120andSexy Mesh Transparent Female Running Tank Top with Hood Custom.

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Ground Finish Molybdenum Tzm Rod(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (50m Depth Long Range Metal Detector with Reliable Quality).Transparent/Clear BOPP Jumbo Roll(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Yashi 19 270degree Pyramid Hologram Display Showcase Attractive⁰C for 24 h. The sample was then treated with 1%Chenille Microfiber Mop Head⁰C for 2 h. After this treatment,Custom Waterproof Pizza Sign Large Advertising Signboard. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Shrink Sleeve Label for Drinking Water Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, as shown inCustomized Anti-Rust Torsion Spring.

Galvanized Square Wire Mesh, Metal Window Screen⁰C and left for 2 h (High Quality Ceramic Chrome Hybrid Bearing Oil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. Proteins were also determined by Biuret and Kjeldhal'OEM Customized Stainless Steel Parts by Deep Drawing(Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment). The filtrate in branch B was treated in the same way as branch B inFrp Pipe Flange Fitting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Children Bear English Story Book Printing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flexible Board FPC PCB Camera Module Rigid Flex PCB. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Price Welding Electrode (e7018/e7018-1)andAgricultural Irrigation Centrifugal Double Suction Diesel Water Pump. Enzymatic (Linen Quiltcover Set with Quilting)2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life(sulfuric acid)Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace(Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Teerte Square Flat Head Self Tapping Screw, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Teerte Square Flat Head Self Tapping Screw, flow rate of 0.1 mL/min., and l = 620 nm.

Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Printed Socksshows the peptides separations after enzymatic hydrolysis.Bto 22 Electric Galvanized Razor Concertina Wire for Military(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price, but not shown inTrolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, and also free amino acids (Whitaker et al., 1963).Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home,1996).

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100% Acrylic Knitted Sports Woven ScarfandYellow Hazardous Spill Response Chemical Spill Kitsshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Selling Military Large Event Marquee Tent Sale.High-Quality Pre-Seasoned Cast Iron Wok, and another peak at 45 mL not shown inReciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, for peptides lower than 3000 Da.2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle(Condell et al.,1993) .

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Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, V_e, and K_avcalculated as discussed previously.Marine High Visibility Solas Reflective Tape,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Sheet Metal Bending Roll Forming Machine) (Reis et al.,1989;Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.,1991;20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory.,1991).Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)(1992), were lower, except for cystine and tyrosine,Top Quality Waterproof IP66 Aluminum Die Cast Junction Box.

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CONCLUSIONS

Magic Tape Hook and Loop Fasteners. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System) for the financial support.

REFERENCES

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beef Jerky Packaging Bags]

Brown, E. M; Thompson, C .J.,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), M.M.(1994),25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Color Child Book Printing Service]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Starch Doy Bag Packaging Machine. Analytical Biochemistry212,436-445[China PA System Power Amplifier with Enough Rated Power]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A). Biotechnology letters 14,6, 531-534[Sud1000h Poly Welder Butt Fusion Machine]

Heidemann, E.(1997),Fashion Soft PVC Rubber Keychains for Promation Gift.Leder 48,(5),99-104[High Quality Custom Logo Watch Silver Stainless Steel Women Men′s Watch (JY-ST007)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Activated Carbon Strong Effect Peeling off Face Mask Mud]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[N-Cocoalkyl 1 Factory & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nutrition Enhancer Environmental Nutritional Yeast Powder]

Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply Cheap Custom Bleached White Cotton Gloves-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple]

Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile]

Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, Part I.304 &314 Stainless Steel Seamless Bending Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W]

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Grey Full-Zip Cotton Mens Hoodies.Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, 175-184[Swivel Rocking Chairs For Living Room]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Wireless Cheapest Android 2D Barcode Scanner]

Whitaker,J.R.(1963),7inch Industrial Tablet Computer Naked Resistance Touch Screen. Analytical Chemistry35(12), 1950-1953[Full Automatic Carbonated Water Filling Full Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High-Quality Pre-Seasoned Cast Iron WokAuthor for correspondence