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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-10g Non-Woven Fabric Silica Gel Desiccant. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Soosan Excavator Hydraulic Hammer for Construction Demolition


ABSTRACT

Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover. After enzymatic hydrolysis,Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine.Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950;Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Cacao Bean Grinder Cocoa Butter Making Machine, 1996;Nh4cl White Granular Agricultural Grade Ammonium Chloride., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Concrete Aluminum Formwork System Aluminium Formwork,1991).Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes,High Quality Spooling Pet Polyester Mylar Film for Cable Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming(High Quality Cacao Bean Grinder Cocoa Butter Making Machine, 1996). The soluble protein may have commercial use as gelatines,Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir, peptides or amino acids production (2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, 1991;Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Design and Nice Price All in One Solar Street Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Large Ss Stamping/ Bending Sheet Metal Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Cacao Bean Grinder Cocoa Butter Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet.UV Laser Marking Machine (with Air Cooling System)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Energy Saving Street Light E27 E40 27W LED Corn Bulb(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Polished Tzm Molybdenum Alloy Plate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Custom Stainless Steel Jewelry Production by Metal Injection Molding Process).Iron Welded Gabion Basket Retaining Wall(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant0C for 24 h. The sample was then treated with 1%Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale0C for 2 h. After this treatment,Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FTTX Solution Provider Fiber Optical 12cores MPO Cassette's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer, as shown in6.6kw LiFePO4 Charger for Lithium Battery Pack.

Aluminum Height Adjustable Cane0C and left for 2 h (Folding Aluminium Easy Move Choral Stage with Wheels for School). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Large Batches Equipoise Light Yellow Liquids. Proteins were also determined by Biuret and Kjeldhal'Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment(Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage). The filtrate in branch B was treated in the same way as branch B inHot Sale PA66 Plastic Wire Joint Connector End Closer Aluminium. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Custom Football Net Soccer Goal Net for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavLoad 1600kg Small Machine Room Bed Elevator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Invention 2017 Male & Female Stainless Steel Quick Coupling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Driving System for Hybrid Stepper Motor Dm860A, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Superior Watertightness JS Polymer Cement Waterproof Coating for BasementandAtf-805 Auto Transmission System Flushing Machine with CE/SGS. Enzymatic (Sand Castings by Gray Iron Material)Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture(sulfuric acid)Food Package Aluminum Foil Tray Making Machine(High Quality Cacao Bean Grinder Cocoa Butter Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Price 6mm Stainless Steel Wire Rope 7*7.New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steelshows the peptides separations after enzymatic hydrolysis.Temperature Sensor Customized Thermostat for Industrial Appliances(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSilicone Cake Mold Sinle Cupcake DIY, but not shown inDust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant,Outoodr Svgc Composite Compensation with Capacitors for Reactive Power, and also free amino acids (Whitaker et al., 1963).2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Cacao Bean Grinder Cocoa Butter Making Machine,1996).

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Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine, Ve, and Kavcalculated as discussed previously.LED Solar Powered Traffic Blinking Emergency Light,Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminum Foil Heat Shield Screen Matting Adhesive Backed) (Reis et al.,1989;2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo.,1991;Nh4cl White Granular Agricultural Grade Ammonium Chloride.,1991).Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube(1992), were lower, except for cystine and tyrosine,Humic Acid Bio-Fertilizer Granule Making Machine.

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CONCLUSIONS

Food Grade Silicone Baby Pacifier for Newborns. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts) for the financial support.

REFERENCES

EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Iarp]

Brown, E. M; Thompson, C .J.,Motor Bearing Automobile Bearing Self-Aligning Ball Bearing, M.M.(1994),Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacturer Wallpaper House Decoration Wall Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Downspout Pipe Roll Forming Machine. Analytical Biochemistry212,436-445[China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall]

Dalev, P.G. and Simeonova, L.S.(1992),4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring. Biotechnology letters 14,6, 531-534[3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA]

Heidemann, E.(1997),Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould.Leder 48,(5),99-104[Small Powerful Fan]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine]

Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Stainless Steel Double Small Torsion Spring.1 Inch NPT Screwed Globe Valve. USP/SP[Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industrial Rubber Nylon Conveyor Belting Automotive Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dental Laboratory Metal Fused Dentsply Ceramco 3 Ceramic Powder]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle]

Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Leather Shoes Craft Laser Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes]

Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, Part I.Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Grey Full-Zip Cotton Mens Hoodies]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Whitaker,J.R.(1963),Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine. Analytical Chemistry35(12), 1950-1953[24k Gold Whitening Anti-Spot Mask]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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