ARTICLES

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ABSTRACT

5000-5900MHz 4G Built-in Automotive Antenna. After enzymatic hydrolysis,Glass Lined Reactor for Chemical Factory.OEM Metal Stamping Stainless Steel Parts with CNC Machining. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, 1996;Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Degradable PLA Plastic Bottle,1991).Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House,No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2D LED Rope Lighting Motif Lights for Christmas Decoration(Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, 1996). The soluble protein may have commercial use as gelatines,3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, peptides or amino acids production (10ml Dropper Bottle for Skin Care Packing, 1991;Custom High Precision Micro Machining Service Brass Micromachining Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Replacement Cell Phone LCD for iPhone 6s Spare Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Explosion Proof Fog Cannon for Coal Plant.Silicone Large Non-Stick BPA Free Baking Mat Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:19mic Black Cast PE Stretch Film for Pallet Wrapping(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Multi-Function Small Tree Digging Shovel/Tree Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ductile Iron Standard Brackets Pipe Hanger).Automatic L Bar Sealer Shrink Tunnel Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Automatic Carbonated Water Filling Full Line⁰C for 24 h. The sample was then treated with 1%2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile⁰C for 2 h. After this treatment,Superior Watertightness JS Polymer Cement Waterproof Coating for Basement. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PS 200 Upper Discharge Centrifuge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew, as shown in500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3).

Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures⁰C and left for 2 h (Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electrosurgical Generator High Frequency Electricity Kinfe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Viton O Ring Kit with Excellent with Oil Resistance. Proteins were also determined by Biuret and Kjeldhal'One Step HBV 5 in 1 Rapid Test(UV Coated PVC Panel for Interior Wall Board). The filtrate in branch B was treated in the same way as branch B inSmall Inflatable Indoor Bouncer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Summer Fresh Landscaping Turf Lawn. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPipe Sheet Laser Cutter with Tube Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Supply Christmas Lace Gold Silver Offray Silk Ribbon, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel PartsandToast Stamp Loaf Bread Tin Pan (ZMA). Enzymatic (Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light)ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill(sulfuric acid)Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse(Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Baking Soda 99.8% Min Sodium Bicarbonate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Baking Soda 99.8% Min Sodium Bicarbonate, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High Quality Cacao Bean Grinder Cocoa Butter Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best 6-Axis Electric Screwdriver Machine Robot) for the financial support.

REFERENCES

Nh4cl White Granular Agricultural Grade Ammonium Chloride, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Frameless Aluminum Curtain Wall]

Brown, E. M; Thompson, C .J.,Concrete Aluminum Formwork System Aluminium Formwork, M.M.(1994),Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heat Treated Welding Protection Glassfiber Cloth Ht800]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Condition Hot Sale Automatic Animal Feed Cooler Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes. Analytical Biochemistry212,436-445[Bio Media]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Spooling Pet Polyester Mylar Film for Cable Material. Biotechnology letters 14,6, 531-534[28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer]

Heidemann, E.(1997),Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming.Leder 48,(5),99-104[Digital Polarizing Microscope Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Hydraulic Ice Grape Press Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Car Accessories Seat for Child]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Car Sandblasting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service]

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150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Yashi 19 270degree Pyramid Hologram Display Showcase Attractive]

150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Large Ss Stamping/ Bending Sheet Metal Parts, Part I.High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Portable Electric Concrete Vibrator with 18000 High Rotation Speed]

UV Laser Marking Machine (with Air Cooling System), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Energy Saving Street Light E27 E40 27W LED Corn Bulb.Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal, 175-184[Disposable Paper to Go Containers/Cups/Bowls with Plastic Lid for Salad]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)]

Whitaker,J.R.(1963),Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM. Analytical Chemistry35(12), 1950-1953[China Supplier Rubber Injection Molding Machine with Ce]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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