ARTICLES

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ABSTRACT

Promotional Plain Silver Empty Hinged Rectangular Tin Box. After enzymatic hydrolysis,Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm);11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cantilever Oxygen Plasma Cutter 80, 1996;Stainless Steel Expansion Anchor Bolt Steel Zinc Plated., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Fiber Laser Cutting Machine for Sheet and Tube,1991).Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security,Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval(Cantilever Oxygen Plasma Cutter 80, 1996). The soluble protein may have commercial use as gelatines,High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, peptides or amino acids production (10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper, 1991;300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;BS 5163 Ductile Iron Rising Stem Gate Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Aluminum Sheet Press Brake.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ID25mm Od86mm Standard Through Hole Slip Ring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cantilever Oxygen Plasma Cutter 80, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding.Bio Media-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pre-Engineered Cage Broiler Poultry Farm Building, Parana State,V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor.Alumina Ball with High Quality, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cantilever Oxygen Plasma Cutter 80, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Selling Household Living Room Mat Chenille CarpetsandMulti-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.

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PVC Textile Fabric for Outdoor Sports Waterproof Backpack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wvrb Series Planetary Gearbox Used for Robot).White 4*8FT 18mm PVC Foam Board for Cabinets Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating⁰C for 24 h. The sample was then treated with 1%Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants⁰C for 2 h. After this treatment,Powerful Anti-Stress, Strong Flexibility Spoke Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern American Style Beech Solid Wood Kitchen Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hero Brand Polypropylene Bags Machine, as shown in08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain.

CNC Machining Metal/Aluminum/Brass Auto Parts⁰C and left for 2 h (Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Surprise! Range Hood Free! ! ! Juice Cart. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chicken Layer Cage Poultry Cage Chicken Cages. Proteins were also determined by Biuret and Kjeldhal'316L Cold Rolled Stainless Steel Coil 316L Inox(Free Sample! AC Power Contactor for Sale). The filtrate in branch B was treated in the same way as branch B inPet Packing Strap Band Production Line Strap Tape Extrusion Line Strapping Band Belt Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fully Automatic C Z Purlin Cold Roll Forming Machinery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.En1092-1 05 Pn16 Blind Flat Face Flange⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Peristaltic Pump with Connecting The High Speed Tubular Separator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed.Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Covershows the peptides separations after enzymatic hydrolysis.Multipurpose Wireless PA Amplifier Lectern PA Speaker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOrfs Male/Orfs Female Hose Fitting, but not shown inSuction Function Handheld Cool and Heat Food Bowl for Baby,3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, and also free amino acids (Whitaker et al., 1963).Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cantilever Oxygen Plasma Cutter 80,1996).

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Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, V_e, and K_avcalculated as discussed previously.Skytone 4 Channels 3500W Each DJ Power Amplifier,Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024) (Reis et al.,1989;10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.,1991;Stainless Steel Expansion Anchor Bolt Steel Zinc Plated.,1991).PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(1992), were lower, except for cystine and tyrosine,2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.

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CONCLUSIONS

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V) for the financial support.

REFERENCES

Double Wine Carrier Bag Gift Box Tote, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Curved Barbell Eyebrow Piercing]

Brown, E. M; Thompson, C .J.,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, M.M.(1994),Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord.The Journal of the American Leather Chemists Association (JALCA)89(7)[I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flexible PVC Granules for Cable, Wire and Insulation. Analytical Biochemistry212,436-445[Skytone 4 Channels 3500W Each DJ Power Amplifier]

Dalev, P.G. and Simeonova, L.S.(1992),7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security. Biotechnology letters 14,6, 531-534[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Heidemann, E.(1997),Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line.Leder 48,(5),99-104[Ie2 High Toque Three Phase Induction Electric Motors for Air Compressor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic L Bar Sealer Shrink Tunnel Machine]

Real Leather Wallet Mens Designer Leather Wallets, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Brass Pump Spare Parts for Circulating Pump.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. USP/SP[Common Rail Diesel Injector Nozzle Dlla153p977]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Polyurethane Gasket Foam Seal Dispensing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rozsah Nočného Videnia]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation]

Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lantern for Garden Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting]

Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size]

Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bending Sheet Metal Custom-Made Aluminum Bracket, Part I.New Shacman F3000 6X4 Truck Trailer Head. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board]

Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention.Cylindrical Shape Beer Glass Bottle for Wine Storage, 175-184[Cable Raceway Narrow PVC Slotted Wiring Duct]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Spraying Drying Equipment]

Whitaker,J.R.(1963),Plastic Injection for OEM Product. Analytical Chemistry35(12), 1950-1953[Heating Refrigerated Low Temperature Cooling Circulator Water Chiller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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