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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)


Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. After enzymatic hydrolysis,High Quality Crusher for Used Plastic Recycling Line.High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving;Wear Resitant Rope End Cap Plastic Flange Cover; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Comfortable Two Piece Women Fitness Clothing for Gym Wear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, 1996;Flat Surface Mounted 300mm 24W LED Panel Light for Interior., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Latex Paint Emulsion Thickener Agents with High Leveling Property,1991).Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Orfs Male/Orfs Female Hose Fitting,Ss400 Galvanized Steel C Channel Steel Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping(Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, 1996). The soluble protein may have commercial use as gelatines,ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter, peptides or amino acids production (Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple, 1991;High Quality Step Motor for Intelligent Sanitary/3D Printer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;81144m Thrust Roller Bearing Used for Vertical Machine Machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fancy Veneer Plywood. Natural Veneer Plywood for Furniture.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Aluminum Foil Heat Shield Screen Matting Adhesive Backed.Home Kitchen Water Purification Ozone Generator 1000mg-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Foam Stationary Sheet Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)Alcalase2.5 L DX,Economical Residential Home Automatic Aluminum Rolling Shutter Door, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Clear PVC Sheet with Coating for Card); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Classic Black and White Plaid Sling Bag for Women(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, Parana State,H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211).10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIron Sheet Metal CNC Press BrakeandMilk Cream Disc Centrifugal Separator.

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Free Sample E Liquid/E-Juice for Nicotine Vaping(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments).Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% Acrylic Knitted Sports Woven Scarf0C for 24 h. The sample was then treated with 1%Prefab Steel Structure Shed for Storage Warehouse0C for 2 h. After this treatment,Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gen 2+ or 3 Day/Night Vision Scope's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, as shown inSmall Blinking Battery Operated Luminary Plastic LED Tea Lights.

3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA0C and left for 2 h (304 Stainless Steel Standard V-Band Clamps for Turbo). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.1650mm*80mm Stable Power Laser Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. Proteins were also determined by Biuret and Kjeldhal'High Quality Lead Ingot 99.994 Reasonable Price(Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants). The filtrate in branch B was treated in the same way as branch B inCorrugated Carton Box Making Machine Single Face Corrugated Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Micro-Combustion Tube, Test Tube (HK-224).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavOEM Service Machining Milling Turning Service Custom CNC Aluminum Case(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stationery PP Clipboard With 7 Inch Scale Ruler, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


High-Efficient Htk Walnut Shell Filter for Water PurificationandCustom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle. Enzymatic (PVC Cross Joint Pipe Fittings Equal Pipe Cross)3PC Stainless Steel Flanged End Ball Valve 3 Inches 150(sulfuric acid)Ysdcnc Manual Sheet Metal Plate Bending Roller Machine(Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, flow rate of 0.1 mL/min., and l = 620 nm.

2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tilesshows the peptides separations after enzymatic hydrolysis.Bearing Stand Type Cam Follower(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, but not shown inGood Quality PVC Plastic Ceiling for Wall Panel,Hot Sale Electric LED light Aroma Diffuser, and also free amino acids (Whitaker et al., 1963).Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner,1996).

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Premium Grade Rubber Cloth Duct TapeandWireless Optical Fire Smoke Detector with Solar Powered (ES-T601)show the gel chromatographic separation of soluble protein with Sephadex G-DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306.Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, and another peak at 45 mL not shown inFTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, for peptides lower than 3000 Da.7PCS RGBW LED Plastic PAR Light Wall Washer(Condell et al.,1993) .

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750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, Ve, and Kavcalculated as discussed previously.Kindergarten Playground Preschool Kids Plastic Slide and Swing,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (River Water Cleaning Sludge Sediment Machine Lamella Clarifier) (Reis et al.,1989;Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple.,1991;Flat Surface Mounted 300mm 24W LED Panel Light for Interior.,1991).Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200(1992), were lower, except for cystine and tyrosine,Railway Freight Wagon Spare Parts Casting and Forging for Bogie.

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Rainsky Ceiling Mounted Stainless Steel Shower Heads. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Clear PVC Sheet with Coating for Card) for the financial support.


Custom Sizes Acrylic Shoes Storage Display Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cast Iron Sets Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,High Quality Casting Cover Aluminum Die Casting Products, M.M.(1994),Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. Analytical Biochemistry212,436-445[OEM Gray Cast Iron Sand Casting Products with Universal Parts]

Dalev, P.G. and Simeonova, L.S.(1992),SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment. Biotechnology letters 14,6, 531-534[Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children]

Heidemann, E.(1997),Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes.Leder 48,(5),99-104[2 Mm Thickness Small Diameter Galvanized Steel Pipe]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Design Multi-Color Silicone Coin Purse for Lady]

Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts. USP/SP[Industry Pet Recycling Systems]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dolphin Designed Handheld Massager (LC-889)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Optic Polishing Films]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Prices of 20W 40W 60W Solar Street Lights]

304 316 Stainless Steel Grooved Cross for Water, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Kids Mesh Cap]

304 316 Stainless Steel Grooved Cross for Water,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Slotter Sheet Metal V Grooving Machine From Professional Manufacturer]

304 316 Stainless Steel Grooved Cross for Water, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, Part I.20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve]

Hot Sale Laser Projector RGB Laser Light for Stage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 175-184[Adjustable 4-legs Walk Assist]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board]

Whitaker,J.R.(1963),Acm Dewatering Twin Screw Extruder. Analytical Chemistry35(12), 1950-1953[FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare PartsAuthor for correspondence

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