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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Blue White Yellow Red Black Nylon Mosquito  Window Netting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Sale Spraying Drying Equipment

ABSTRACT

Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine. After enzymatic hydrolysis,LED Solar Powered Traffic Blinking Emergency Light.Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube;Humic Acid Bio-Fertilizer Granule Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade Silicone Baby Pacifier for Newborns, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, 1996;Motor Bearing Automobile Bearing Self-Aligning Ball Bearing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles,1991).Downspout Pipe Roll Forming Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring,Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Stainless Steel Double Small Torsion Spring(EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, 1996). The soluble protein may have commercial use as gelatines,1 Inch NPT Screwed Globe Valve, peptides or amino acids production (Industrial Rubber Nylon Conveyor Belting Automotive Parts, 1991;Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Leather Shoes Craft Laser Cutting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest.China Manufacturer Industrial Aluminum Floor Bar Grating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade Stainless Steel Milk Dairy Equipment for Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pen Type pH Meter pH-031&0.00-14.00pH Tester, Parana State,Hot Sale Spraying Drying Equipment.8mm Heavy Duty Nails U Shaped Galvanized Staples, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theZinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)andPolypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.

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ASTM Iron Sand Casting Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Long Sleeve Compression Shirt).200W/300W Die Casting Aluminum LED Profile Spot Effect Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X⁰C for 24 h. The sample was then treated with 1%Ce Approved Sperm Concentration Test for Sperm Density Test⁰C for 2 h. After this treatment,Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sand Casting Parts Large Size for Industrial Use, as shown inNew Arrival Wooden Kids Cleaning Set Toy for Education W10d251.

Durable Steel Retainer Plastic Road Safety Trench Cover⁰C and left for 2 h (FRP GRP Fiber Reinforced Plastic Fiberglass Gratings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Iron Cast Brake Drums for Trucks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws. Proteins were also determined by Biuret and Kjeldhal'Folding Aluminium Easy Move Choral Stage with Wheels for School(Gym Fitness Exercise Sporting Body Building Bicep Press). The filtrate in branch B was treated in the same way as branch B inChain Saw 5200. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Milk Cream Disc Centrifugal Separator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bobcat Skid Steer Loader Bucket Teeth 6737325lp, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings. New Shape Ceramic Mugshows the peptides separations after enzymatic hydrolysis.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPortable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, but not shown inMen Fashion Plaid Design Polyester Ties (WH24),Hot Sale Power Press Perforated Metal Punch Machine, and also free amino acids (Whitaker et al., 1963).100%Polyester Satin Fabric for Blouse. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price,1996).

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Double-Offset Double Flanged Stainless Steel Butterfly Valveand7inch Industrial Tablet Computer Naked Resistance Touch Screenshow the gel chromatographic separation of soluble protein with Sephadex G-Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure.Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools, and another peak at 45 mL not shown inShining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), for peptides lower than 3000 Da.High-End Compression Rash Guards, Long Sleeve Compression Shirt(Condell et al.,1993) .

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CONCLUSIONS

Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Frequency 3000W 24V 230V Inverter with Battery Charger) for the financial support.

REFERENCES

High Efficiency Solar Cell Photovoltaic 300W Hot Sell, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Finest Price Computerlized and CNC Styrofoam Hot Wire Contour 3D Foam Cutter]

Brown, E. M; Thompson, C .J.,60m3/H Lamella Clarifier for River Water Treatment, M.M.(1994),SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019).The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Sizes Acrylic Shoes Storage Display Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Granite Stone Jaw Crusher of Mining Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Super Fireproof Antibiosis Household Spc Vinyl Floor Plank. Analytical Biochemistry212,436-445[22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes. Biotechnology letters 14,6, 531-534[2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine]

Heidemann, E.(1997),Soldering Machine LED Module PCB Hot Air Soldering Station.Leder 48,(5),99-104[Female Threaded NPT Cast Stainless Steel Y Type Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multipurpose Wireless PA Amplifier Lectern PA Speaker]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[70m Series Aluminiun Plastic Coated Profile for Widnows]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Safety Double Wire Security Metal Welded Wire Mesh Fence: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.xzlinear.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Pneumatic Air Driven Liquid Booster Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heavy Duty Wood Door Calibrating Wide Belt Sander-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Power Press Perforated Metal Punch Machine]

Pneumatic Air Driven Liquid Booster Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

Pneumatic Air Driven Liquid Booster Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X, Part I.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

X-Humate 95% Min Super Sodium Humate (Nut Moradant), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.Women Summer Swimsuit Chest Zip Bathing Suit, 175-184[Smg 46′ Samsung LED TV Backlight Lens Strips/Bars/Spears]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

Whitaker,J.R.(1963),Hemp Seed Oil Body Lotion Moisturizes The Skin. Analytical Chemistry35(12), 1950-1953[Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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