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ABSTRACT

All Weather Rattan Handwoven Swing Outdoor Home. After enzymatic hydrolysis,Pond Self Priming Booster suction  Water Pump with Pressure Switch.Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace;for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), 1996;Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation,1991).0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,125kHz RFID Hotel Key Card with T5577 Chip,USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag(School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), 1996). The soluble protein may have commercial use as gelatines,Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, peptides or amino acids production (Wholesale High Pressure 80L Nitrogen Gas Cylinder, 1991;10mm Red Green Yellow Warning China Supplier Signal Lamp, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Thermostat Electric Heating Element for Solar Water Heaters.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Phillip Countersunk Head Screw Hot DIP Galvanized. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heat Shrink Automatic Flow Wrapping Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Different Size Glass Test Tubes, High Quality Laboratory Test Tubes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Waterproof Pizza Sign Large Advertising Signboard).Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Frame Type Rubber Hydraulic Press Machine⁰C for 24 h. The sample was then treated with 1%Kitchen Metal Dish Storage Rack (JP-SC984C)⁰C for 2 h. After this treatment,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)AVR Se350 Automatic Voltage Regulator for Marathon Alternator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, as shown inFine Bubble Diffuser for Air Purifier.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Straight Pneumatic Fitting Quick Connect Air FittingsandCross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. Enzymatic (Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon)High Quality Ceramic Chrome Hybrid Bearing Oil(sulfuric acid)Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown(School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, flow rate of 0.1 mL/min., and l = 620 nm.

Digger Arm Small Wheel Loader (HQ908) with CE. Coppershows the peptides separations after enzymatic hydrolysis.15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Customized Stainless Steel Parts by Deep Drawing, but not shown inOne Arm Motorized Multifunction Hospital Equipment for China Supply,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, and also free amino acids (Whitaker et al., 1963).Water Based Emulsion Paint Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056),1996).

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CONCLUSIONS

Side Pack Manual Gas Engine Power Brush Cutter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers) for the financial support.

REFERENCES

Punching Bending Welding Steel Work Fabrication, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.zirae.com]

Brown, E. M; Thompson, C .J.,Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, M.M.(1994),Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Food Grade Silicone Baby Pacifier for Newborns]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1 Ton Chain Pulley Block with Geared Trolley. Analytical Biochemistry212,436-445[Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)]

Dalev, P.G. and Simeonova, L.S.(1992),Powder Coating Polyester Resin with Factory Price. Biotechnology letters 14,6, 531-534[Quality Shaper Machine Bc6085 Shapping Machine]

Heidemann, E.(1997),Auto Plastic Part Hardware Plastic Injection Mold.Leder 48,(5),99-104[Prefabricated House and Pre Engineered Steel Structure Building]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ozonated Water for Vegetables and Fruits Washing Ozone Generator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Blue White Yellow Red Black Nylon Mosquito  Window Netting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Surgical Suture Needle With Thread]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Concrete Aluminum Formwork System Aluminium Formwork]

180W 1850mm*80mm Reci Laser Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Factory Balloon Padding Textile Machinery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Worm Gear Fluorine Lined Butterfly Valve Flanged Type]

180W 1850mm*80mm Reci Laser Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole]

180W 1850mm*80mm Reci Laser Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, Part I.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W]

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Concert Stage Equipment Aluminum Stage Portable Stage.Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, 175-184[www.puyerinternationalgroup.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)]

Whitaker,J.R.(1963),22*30mm 12SMD 5050 LED Reading Light for Car. Analytical Chemistry35(12), 1950-1953[ Highly Stable Performance Disposable Blood Lancet with Cheap Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galvanized Bar GratingAuthor for correspondence