ARTICLES

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ABSTRACT

Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal. After enzymatic hydrolysis,Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681).High Quality Mini LED Flood Light SMD Flood Light 50W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ferrite Core for Power Transformer (Ee16e);Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Wallpaper House Decoration Wall Paper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flexible Magnetic Rubber Sheet Soft Magnetic Tape, 1996;Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Wine Bottle Opener K020,1991).Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery,China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Spine Shape Landscaping Artificial Turf Grass(Flexible Magnetic Rubber Sheet Soft Magnetic Tape, 1996). The soluble protein may have commercial use as gelatines,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, peptides or amino acids production (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1991;Diesel Engine Type Zzs Sand Vibrating Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bluetooth Smart Watch with Heart Rate Monitor for Men 72087.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Trendy High Quality 925 Stering Silver Tennis Glod Charm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flexible Magnetic Rubber Sheet Soft Magnetic Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.Stand 2.5t Electric Pallet Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, Parana State,Transport System China Manufacturer for Table Belt Conveyor.Vertical Type Automatic Box Erector Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Flexible Magnetic Rubber Sheet Soft Magnetic Tape, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)andNylon Balance Bike with Water Transfer Printing Malaysia.

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Air Cooled Scroll Type Water Chiller for Industrial Process(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam).Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090⁰C for 24 h. The sample was then treated with 1%Rotary Vibrating Sieve Machine for Compost⁰C for 2 h. After this treatment,I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa, as shown inFabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.

Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet⁰C and left for 2 h (SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Condoms/Sex Toys/Capsule Toy Vending Machine. Proteins were also determined by Biuret and Kjeldhal'Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)(FSTN Type Monochrome Graphic Display Cog LCD with Backlight). The filtrate in branch B was treated in the same way as branch B inVapor Tight Barn Lights. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Type 40kgs Movable Ice Maker for Store Use. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMotorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2*2 4G Lte MIMO Antenna Rrouter/CPE⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Galvanized Coated Barbed Wire (MT-BW013), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bagshows the peptides separations after enzymatic hydrolysis.Cute Clear Fanny Pack Stadium Security PVC Waist Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIrrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming, but not shown inAluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK,Personalized Coffee Mug Cup with Custom Logo, and also free amino acids (Whitaker et al., 1963).100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Flexible Magnetic Rubber Sheet Soft Magnetic Tape,1996).

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700mm RP HP UHP High Quality Graphite Electrode, V_e, and K_avcalculated as discussed previously.Prefab Building Insulated Interior Concrete Partition Walls,Disk Filter System with Automatic Backwash Valves. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminium Unitized Curtain Wall for Office Building) (Reis et al.,1989;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.,1991;Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass.,1991).Eco-Friendly Recycling PS Foam Container Production Line(1992), were lower, except for cystine and tyrosine,Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve.

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CONCLUSIONS

Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Men Fashion Plaid Design Polyester Ties (WH24)) for the financial support.

REFERENCES

High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bnc Female Cable]

Brown, E. M; Thompson, C .J.,Jacquard Football Fans Acrylic Sports Warmer Scarf, M.M.(1994),Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.The Journal of the American Leather Chemists Association (JALCA)89(7)[Milk Cream Disc Centrifugal Separator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Blinking Battery Operated Luminary Plastic LED Tea Lights. Analytical Biochemistry212,436-445[Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments]

Dalev, P.G. and Simeonova, L.S.(1992),Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine. Biotechnology letters 14,6, 531-534[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

Heidemann, E.(1997),Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole.Leder 48,(5),99-104[10 Inch Advertising Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[304 Stainless Steel Standard V-Band Clamps for Turbo]

Effluent Treatment Ferrous Sulphate Heptahydrate 98%, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket.High Precision Plastic Mold Manufacturer in China. USP/SP[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High-Efficient Htk Walnut Shell Filter for Water Purification]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mango Drying Machine/Solar Fruit Drying Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.jssintering.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Mineral Industry Magnet Ore Ball Milling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality PVC Plastic Ceiling for Wall Panel]

Mineral Industry Magnet Ore Ball Milling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

Mineral Industry Magnet Ore Ball Milling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, Part I.Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Premium Grade Rubber Cloth Duct Tape]

Blasting Cleaning Shot Peeling Machine Tumble Belt Type, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine.Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, 175-184[LED Explosion Proof Flashlight Waterproof Portable Searchlight]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Whitaker,J.R.(1963),Chemical Plant Transfer Industrial Water Pump. Analytical Chemistry35(12), 1950-1953[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Price 6000 Series Industrial Extruded Aluminum TubeAuthor for correspondence