ARTICLES

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ABSTRACT

High Safety Double Wire Security Metal Welded Wire Mesh Fence. After enzymatic hydrolysis,Pneumatic Air Driven Liquid Booster Pump.Heavy Duty Wood Door Calibrating Wide Belt Sander. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.X-Humate 95% Min Super Sodium Humate (Nut Moradant), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, 1996;Women Summer Swimsuit Chest Zip Bathing Suit., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hemp Seed Oil Body Lotion Moisturizes The Skin,1991).SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal,UTP CAT6 48 Ports Vertical Termination Patch Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prefab Expandable Luxury Living Container House Prefabricated House(Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, 1996). The soluble protein may have commercial use as gelatines,Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, peptides or amino acids production (Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1991;Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Hexagonal Wire Mesh Gabion / Gabion Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Must 4kw 2HP Pump Power Inverter Ags Bts Available.Hot Sale Small Steel Silo for Poultry Feed-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Price Nasal Paste Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase).OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ms Gi Pipe Dn15~Dn400 Gi Pipe List⁰C for 24 h. The sample was then treated with 1%Low-E Insulating Glass Curtain Wall⁰C for 2 h. After this treatment,Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Design Underwear for Men Cotton Mens Boxer Briefs, as shown in4-14 Manual Staple Gun with Staples for Hand Tools.

Nylon Balance Bike with Water Transfer Printing Malaysia⁰C and left for 2 h (Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti Slip Rubber Mat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap. Proteins were also determined by Biuret and Kjeldhal'Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor(Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant). The filtrate in branch B was treated in the same way as branch B inAPI Stainless Steel CF8 (M) /CF3 (M) Flanged Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Car Accessories Seat for Child⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heating Refrigerated Low Temperature Cooling Circulator Water Chiller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Zipper Pouch Pistachio Nuts Premade Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wd190V Handheld Full Body Scanner Hand Held Metal DetectorandOriginal Cummins Diesel Engine Spare Parts Flywheel Ring Gear. Enzymatic (Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer)Colombia Outdoor P5 Pantallas LED Display(sulfuric acid)2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch(Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2010 Used Foton Truck Mixer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2010 Used Foton Truck Mixer, flow rate of 0.1 mL/min., and l = 620 nm.

Single Board 3.7V~24VDC Microwave Detector PCB.Single Board 3.7V~24VDC Microwave Detector PCBshows the peptides separations after enzymatic hydrolysis.Automatic Chicken Nipple Drinker System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, but not shown inYsenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound,Industrial Hydraulic Ice Grape Press Machine, and also free amino acids (Whitaker et al., 1963).Common Rail Diesel Injector Nozzle Dlla153p977. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board,1996).

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Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, V_e, and K_avcalculated as discussed previously.Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01),CE Certificated Wheel Loader (HQ912) with Agricultural Tire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane) (Reis et al.,1989;Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries.,1991;Women Summer Swimsuit Chest Zip Bathing Suit.,1991).Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts(1992), were lower, except for cystine and tyrosine,PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material.

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CONCLUSIONS

Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device) for the financial support.

REFERENCES

Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Adjustable Gas Tube Oil Inside Spring with Release Mechanism 200n]

Brown, E. M; Thompson, C .J.,ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, M.M.(1994),Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100).The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen. Analytical Biochemistry212,436-445[Prefab Building Insulated Interior Concrete Partition Walls]

Dalev, P.G. and Simeonova, L.S.(1992),DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw. Biotechnology letters 14,6, 531-534[ASTM Iron Sand Casting Parts]

Heidemann, E.(1997),Auto Two Piece Folder Gluer for Corrugated Box.Leder 48,(5),99-104[Non Woven Geotextile Used on Ire Mesh Gabion Box/Geotextile Filtration]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3120/17/38 Boltable Crane Rail Clips]

Soldering Machine LED Module PCB Hot Air Soldering Station, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),British Standard 2 Gang 1 Way Wall Light Switch.Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts. USP/SP[Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rechargeable 1.1wh 3.7V 300mAh Lipo Battery Cell]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop]

Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jacquard Football Fans Acrylic Sports Warmer Scarf]

Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, Part I.P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rectangular Stainless Steel Pipe SS304.High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, 175-184[Crusher Plant For Stone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10ml Dropper Bottle for Skin Care Packing]

Whitaker,J.R.(1963),6.6kw LiFePO4 Charger for Lithium Battery Pack. Analytical Chemistry35(12), 1950-1953[China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Viola BowAuthor for correspondence