ARTICLES

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ABSTRACT

Clear PVC Sheet with Coating for Card. After enzymatic hydrolysis,Classic Black and White Plaid Sling Bag for Women.24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100% Acrylic Knitted Sports Woven Scarf, 1996;Prefab Steel Structure Shed for Storage Warehouse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture,1991).Gen 2+ or 3 Day/Night Vision Scope. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved,3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1650mm*80mm Stable Power Laser Tube(100% Acrylic Knitted Sports Woven Scarf, 1996). The soluble protein may have commercial use as gelatines,FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit, peptides or amino acids production (High Quality Lead Ingot 99.994 Reasonable Price, 1991;Micro-Combustion Tube, Test Tube (HK-224), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3W LED Explosion Proof Emergency Exit Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100% Acrylic Knitted Sports Woven Scarf, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.Stationery PP Clipboard With 7 Inch Scale Ruler-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ferrous Sulphate Heptahydrate

Bearing Stand Type Cam Follower(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price).Hot Sale Electric LED light Aroma Diffuser(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner⁰C for 24 h. The sample was then treated with 1%DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306⁰C for 2 h. After this treatment,Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)7PCS RGBW LED Plastic PAR Light Wall Washer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, as shown inTrolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace.

Kindergarten Playground Preschool Kids Plastic Slide and Swing⁰C and left for 2 h (Pet PP Rope and Broom Wire Drawing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. Proteins were also determined by Biuret and Kjeldhal'Railway Freight Wagon Spare Parts Casting and Forging for Bogie(100% Acrylic Knitted Sports Woven Scarf). The filtrate in branch B was treated in the same way as branch B inOEM Offered Reinforced Conveyor Automatic L Sealer and Shrink Tunnel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rainsky Ceiling Mounted Stainless Steel Shower Heads.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Sizes Acrylic Shoes Storage Display Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Casting Cover Aluminum Die Casting Products(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Alumina Balls for Hydogen Peroxide. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3PC Stainless Steel Flanged End Ball Valve 3 Inches 150) for the financial support.

REFERENCES

Anti-High Pressure Insulated Pliers with PVC Dipped Handles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2751 Silicone Rubber Fiberglass Sleeves]

Brown, E. M; Thompson, C .J.,Hot Selling Electronical Blue Pet Plastic Release Film Price, M.M.(1994),DIN127 Stainless Steel Spring Lock Washer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Activated Carbon Strong Effect Peeling off Face Mask Mud]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. Analytical Biochemistry212,436-445[Nutrition Enhancer Environmental Nutritional Yeast Powder]

Dalev, P.G. and Simeonova, L.S.(1992),New Condition Hot Sale Automatic Animal Feed Cooler Machine. Biotechnology letters 14,6, 531-534[High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple]

Heidemann, E.(1997),Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System.Leder 48,(5),99-104[High Quality and Efficient Combustion UHP Graphite Electrode]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile]

PP Foam Stationary Sheet Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144).Garlic Red Mesh Bag. USP/SP[Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Wireless Cheapest Android 2D Barcode Scanner]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Automatic Carbonated Water Filling Full Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anodized Aluminum Profile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High-Quality Pre-Seasoned Cast Iron Wok]

Ground Finish Molybdenum Tzm Rod, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Transparent/Clear BOPP Jumbo Roll-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress]

Ground Finish Molybdenum Tzm Rod,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Buy Single Item Moving Bee Eye Light Lowly Price]

Ground Finish Molybdenum Tzm Rod, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, Part I.Chenille Microfiber Mop Head. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Silicone Reusable Food Storage Bag China Manufacturer]

Custom Waterproof Pizza Sign Large Advertising Signboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Shrink Sleeve Label for Drinking Water Bottle.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, 175-184[800W Pipe Welding Socket Fusion Machine Plastic Welder 63mm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cast Iron Butterfly Valve with Handles]

Whitaker,J.R.(1963),Galvanized Square Wire Mesh, Metal Window Screen. Analytical Chemistry35(12), 1950-1953[Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

U. S. Type Drop Forged Wire Rope ClipAuthor for correspondence