ARTICLES

Latex Paint Emulsion Thickener Agents with High Leveling Property

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Orfs Male/Orfs Female Hose Fitting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ss400 Galvanized Steel C Channel Steel Price

ABSTRACT

Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping. After enzymatic hydrolysis,ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter.Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fancy Veneer Plywood. Natural Veneer Plywood for Furniture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, 1996;Aluminum Foil Heat Shield Screen Matting Adhesive Backed., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Home Kitchen Water Purification Ozone Generator 1000mg,1991).PP Foam Stationary Sheet Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640),Economical Residential Home Automatic Aluminum Rolling Shutter Door,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Clear PVC Sheet with Coating for Card(OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, 1996). The soluble protein may have commercial use as gelatines,Classic Black and White Plaid Sling Bag for Women, peptides or amino acids production (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, 1991;10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Free Sample E Liquid/E-Juice for Nicotine Vaping., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100% Acrylic Knitted Sports Woven Scarf. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Prefab Steel Structure Shed for Storage Warehouse.Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gen 2+ or 3 Day/Night Vision Scope(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce ApprovedAlcalase2.5 L DX,3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-1650mm*80mm Stable Power Laser Tube); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

High Quality Lead Ingot 99.994 Reasonable Price, Parana State,Ss400 Galvanized Steel C Channel Steel Price.Micro-Combustion Tube, Test Tube (HK-224), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Filmand FRP Profiles.

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Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( MUG).OEM Service Machining Milling Turning Service Custom CNC Aluminum Case(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3W LED Explosion Proof Emergency Exit Light⁰C for 24 h. The sample was then treated with 1%Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making⁰C for 2 h. After this treatment,Stationery PP Clipboard With 7 Inch Scale Ruler. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle, as shown inYellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed.

PVC Cross Joint Pipe Fittings Equal Pipe Cross⁰C and left for 2 h (Bto 22 Electric Galvanized Razor Concertina Wire for Military). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3PC Stainless Steel Flanged End Ball Valve 3 Inches 150. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ysdcnc Manual Sheet Metal Plate Bending Roller Machine. Proteins were also determined by Biuret and Kjeldhal'Betaine Anhydrous 98% Food Grade Good Quality Competitive Price(New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)). The filtrate in branch B was treated in the same way as branch B inHigh Tensisle Strength Wet Tissue. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bearing Stand Type Cam Follower. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Electric LED light Aroma Diffuser(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/7PCS RGBW LED Plastic PAR Light Wall Washer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Speed Automatic Packing Machine (GK-650 BA)and750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. Enzymatic (Kindergarten Playground Preschool Kids Plastic Slide and Swing)Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks(sulfuric acid)Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200(OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Railway Freight Wagon Spare Parts Casting and Forging for Bogie, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Railway Freight Wagon Spare Parts Casting and Forging for Bogie, flow rate of 0.1 mL/min., and l = 620 nm.

Rainsky Ceiling Mounted Stainless Steel Shower Heads.Electric Paint Sprayer Pumpshows the peptides separations after enzymatic hydrolysis.Custom Sizes Acrylic Shoes Storage Display Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFinks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, but not shown in316L Cold Rolled Stainless Steel Coil 316L Inox,High Quality Casting Cover Aluminum Die Casting Products, and also free amino acids (Whitaker et al., 1963).Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating,1996).

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Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Saleand150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panelsshow the gel chromatographic separation of soluble protein with Sephadex G-304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser.SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, and another peak at 45 mL not shown inWholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, for peptides lower than 3000 Da.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes(Condell et al.,1993) .

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Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, V_e, and K_avcalculated as discussed previously.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (90m3 Per Hour Mobile Concrete Pump) (Reis et al.,1989;24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable.,1991;Aluminum Foil Heat Shield Screen Matting Adhesive Backed.,1991).304 316 Stainless Steel Grooved Cross for Water(1992), were lower, except for cystine and tyrosine,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.

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CONCLUSIONS

Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1650mm*80mm Stable Power Laser Tube) for the financial support.

REFERENCES

20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China custom dice]

Brown, E. M; Thompson, C .J.,Hot Sale Laser Projector RGB Laser Light for Stage, M.M.(1994),Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer. Analytical Biochemistry212,436-445[Punching Bending Welding Steel Work Fabrication]

Dalev, P.G. and Simeonova, L.S.(1992),Acm Dewatering Twin Screw Extruder. Biotechnology letters 14,6, 531-534[Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test]

Heidemann, E.(1997),Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).Leder 48,(5),99-104[Electronic Automatic Cinema Ticket Vending ATM Machine Sheet Metal Fabrication]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304 &314 Stainless Steel Seamless Bending Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Quality Good Price China Plastic Injection Molding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ce Certified Welding Turning Rolls Hdtr-3000 for Round Welding]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car]

Pet Hair Remover Glove for Dog, Grooming Gloves Brush, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality & High Speed Straight Line Wire Drawing Machine]

Pet Hair Remover Glove for Dog, Grooming Gloves Brush,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[125kHz RFID Hotel Key Card with T5577 Chip]

Pet Hair Remover Glove for Dog, Grooming Gloves Brush, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Alumina Balls for Hydogen Peroxide, Part I.Anti-High Pressure Insulated Pliers with PVC Dipped Handles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Black Galvanized Head Stainless Steel Screw]

Hot Selling Electronical Blue Pet Plastic Release Film Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DIN127 Stainless Steel Spring Lock Washer.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, 175-184[China Cnc Auto Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard]

Whitaker,J.R.(1963),New Condition Hot Sale Automatic Animal Feed Cooler Machine. Analytical Chemistry35(12), 1950-1953[Spine Shape Landscaping Artificial Turf Grass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sanjing PVC Lining Layflat Fire HoseAuthor for correspondence