ARTICLES

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ABSTRACT

CNC Aluminum Sheet Press Brake. After enzymatic hydrolysis,ID25mm Od86mm Standard Through Hole Slip Ring.Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bio Media;Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Platform Trolley with 150kg Capacity, 4 Castor Wheel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 1996;LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (All Size Colored Tapping Hex Head Self Drill Screw Wood Screw,1991).Pre-Engineered Cage Broiler Poultry Farm Building. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Alumina Ball with High Quality,PVC Textile Fabric for Outdoor Sports Waterproof Backpack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,White 4*8FT 18mm PVC Foam Board for Cabinets Use(Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 1996). The soluble protein may have commercial use as gelatines,Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, peptides or amino acids production (Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants, 1991;Powerful Anti-Stress, Strong Flexibility Spoke Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern American Style Beech Solid Wood Kitchen Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hero Brand Polypropylene Bags Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Machining Metal/Aluminum/Brass Auto Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Surprise! Range Hood Free! ! ! Juice Cart.Chicken Layer Cage Poultry Cage Chicken Cages-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:316L Cold Rolled Stainless Steel Coil 316L Inox(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

En1092-1 05 Pn16 Blind Flat Face Flange, Parana State,BS 5163 Ductile Iron Rising Stem Gate Valve.Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Grade Reusable Silicone Food Storage Bag OEM ManufacturerandSquare Plastic PP Geocomposite Drainage Blind Ditch/Drain.

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Peristaltic Pump with Connecting The High Speed Tubular Separator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating).High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED Bulb Light with Remote Control RGB Christmas Bulb⁰C for 24 h. The sample was then treated with 1%Home Decoration Kids Toy Promotion Eyeglass Plastic Mould⁰C for 2 h. After this treatment,Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Price Gi/PPGI Roofing Sheet in China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, as shown inAdding Water Rice Pulping Machine/Soybean Grinding Machine.

Multipurpose Wireless PA Amplifier Lectern PA Speaker⁰C and left for 2 h (Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. Proteins were also determined by Biuret and Kjeldhal'High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites). The filtrate in branch B was treated in the same way as branch B in5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12ml Round Shape Empty Clear Nail Polish Glass Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%M4X10 Round Head Core Pulling Aluminum Nail. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3W LED Explosion Proof Emergency Exit LightandImirootree 250ml Glass Jar with Lids for Cosmetic Cream. Enzymatic (Double Wine Carrier Bag Gift Box Tote)(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts(sulfuric acid)Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord(Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Flexible PVC Granules for Cable, Wire and Insulation, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Flexible PVC Granules for Cable, Wire and Insulation, flow rate of 0.1 mL/min., and l = 620 nm.

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security. Long Sleeve Compression Shirtshows the peptides separations after enzymatic hydrolysis.Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Arrival Wooden Kids Cleaning Set Toy for Education W10d251, but not shown inFRP GRP Fiber Reinforced Plastic Fiberglass Gratings,Brass Pump Spare Parts for Circulating Pump, and also free amino acids (Whitaker et al., 1963).Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine,1996).

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Bending Sheet Metal Custom-Made Aluminum Bracket, V_e, and K_avcalculated as discussed previously.New Shacman F3000 6X4 Truck Trailer Head,Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell) (Reis et al.,1989;Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.,1991;LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.,1991).Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention(1992), were lower, except for cystine and tyrosine,Cylindrical Shape Beer Glass Bottle for Wine Storage.

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CONCLUSIONS

Plastic Injection for OEM Product. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant) for the financial support.

REFERENCES

HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cable Plastic Wire Plug 2-8pin Waterproof Electrical Connectors]

Brown, E. M; Thompson, C .J.,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, M.M.(1994),Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Relfective Plastic Reflector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Activated Carbon Strong Effect Peeling off Face Mask Mud. Analytical Biochemistry212,436-445[24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Type Price Prefabricated Roof Tile in Sudan. Biotechnology letters 14,6, 531-534[Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool]

Heidemann, E.(1997),Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion.Leder 48,(5),99-104[1000base Sx Sfp Transceiver High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter]

10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Supply 100% Natural Epimedium Extract for Male Health.Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust. USP/SP[Custom Sizes Acrylic Shoes Storage Display Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Granite Stone Jaw Crusher of Mining Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Connector Usb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine]

Top10 1080P H. 264 P2p CCTV WiFi IP Camera, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Multipurpose Wireless PA Amplifier Lectern PA Speaker]

Top10 1080P H. 264 P2p CCTV WiFi IP Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine]

Top10 1080P H. 264 P2p CCTV WiFi IP Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, Part I.Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[70m Series Aluminiun Plastic Coated Profile for Widnows]

1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Color Child Book Printing Service.1.5 Inch High Pressure Petrol/Gasoline Water Pump, 175-184[Fine Sand Washing & Recycling Equipment for Mining]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

Whitaker,J.R.(1963),Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine. Analytical Chemistry35(12), 1950-1953[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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